Layer-by-layer functionalized nanotube arrays: A versatile microfluidic platform for biodetection
We demonstrate the layer-by-layer (LbL) assembly of polyelectrolyte multilayers (PEM) on three-dimensional nanofiber scaffolds. High porosity (99%) aligned carbon nanotube (CNT) arrays are photolithographically patterned into elements that act as textured scaffolds for the creation of functionally c...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | en_US |
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Springer Nature
2017
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| Online Access: | http://hdl.handle.net/1721.1/111955 https://orcid.org/0000-0003-2399-4397 https://orcid.org/0000-0003-4395-523X https://orcid.org/0000-0003-3965-0740 https://orcid.org/0000-0003-1085-7692 https://orcid.org/0000-0001-8323-2779 https://orcid.org/0000-0003-3570-8917 https://orcid.org/0000-0003-3530-5819 |
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| author | Toner, Mehmet Yost, Allison Lynne Shahsavari, Setareh Bradwell, Grinia M. Polak, Roberta Fachin, Fabio Cohen, Robert E McKinley, Gareth H Rubner, Michael F Wardle, Brian L |
| author2 | Massachusetts Institute of Technology. Department of Aeronautics and Astronautics |
| author_facet | Massachusetts Institute of Technology. Department of Aeronautics and Astronautics Toner, Mehmet Yost, Allison Lynne Shahsavari, Setareh Bradwell, Grinia M. Polak, Roberta Fachin, Fabio Cohen, Robert E McKinley, Gareth H Rubner, Michael F Wardle, Brian L |
| author_sort | Toner, Mehmet |
| collection | MIT |
| description | We demonstrate the layer-by-layer (LbL) assembly of polyelectrolyte multilayers (PEM) on three-dimensional nanofiber scaffolds. High porosity (99%) aligned carbon nanotube (CNT) arrays are photolithographically patterned into elements that act as textured scaffolds for the creation of functionally coated (nano)porous materials. Nanometer-scale bilayers of poly(allylamine hydrochloride)/poly(styrene sulfonate) (PAH/SPS) are formed conformally on the individual nanotubes by repeated deposition from aqueous solution in microfluidic channels. Computational and experimental results show that the LbL deposition is dominated by the diffusive transport of the polymeric constituents, and we use this understanding to demonstrate spatial tailoring on the patterned nanoporous elements. A proof-of-principle application, microfluidic bioparticle capture using N-hydroxysuccinimide-biotin binding for the isolation of prostate-specific antigen (PSA), is demonstrated. |
| first_indexed | 2024-09-23T15:20:54Z |
| format | Article |
| id | mit-1721.1/111955 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T15:20:54Z |
| publishDate | 2017 |
| publisher | Springer Nature |
| record_format | dspace |
| spelling | mit-1721.1/1119552022-10-02T02:23:57Z Layer-by-layer functionalized nanotube arrays: A versatile microfluidic platform for biodetection Toner, Mehmet Yost, Allison Lynne Shahsavari, Setareh Bradwell, Grinia M. Polak, Roberta Fachin, Fabio Cohen, Robert E McKinley, Gareth H Rubner, Michael F Wardle, Brian L Massachusetts Institute of Technology. Department of Aeronautics and Astronautics Massachusetts Institute of Technology. Department of Chemical Engineering Massachusetts Institute of Technology. Department of Materials Science and Engineering Massachusetts Institute of Technology. Department of Mechanical Engineering Yost, Allison Lynne Shahsavari, Setareh Bradwell, Grinia M. Polak, Roberta Fachin, Fabio Cohen, Robert E McKinley, Gareth H Rubner, Michael F Wardle, Brian L We demonstrate the layer-by-layer (LbL) assembly of polyelectrolyte multilayers (PEM) on three-dimensional nanofiber scaffolds. High porosity (99%) aligned carbon nanotube (CNT) arrays are photolithographically patterned into elements that act as textured scaffolds for the creation of functionally coated (nano)porous materials. Nanometer-scale bilayers of poly(allylamine hydrochloride)/poly(styrene sulfonate) (PAH/SPS) are formed conformally on the individual nanotubes by repeated deposition from aqueous solution in microfluidic channels. Computational and experimental results show that the LbL deposition is dominated by the diffusive transport of the polymeric constituents, and we use this understanding to demonstrate spatial tailoring on the patterned nanoporous elements. A proof-of-principle application, microfluidic bioparticle capture using N-hydroxysuccinimide-biotin binding for the isolation of prostate-specific antigen (PSA), is demonstrated. National Science Foundation (U.S.) (Award DMR-0819762) 2017-10-23T15:59:45Z 2017-10-23T15:59:45Z 2017-10-23 Article http://purl.org/eprint/type/JournalArticle 2055-7434 http://hdl.handle.net/1721.1/111955 Yost, Allison L. et al. “Layer-by-Layer Functionalized Nanotube Arrays: A Versatile Microfluidic Platform for Biodetection.” Microsystems & Nanoengineering 1 (December 2015): 15037 https://orcid.org/0000-0003-2399-4397 https://orcid.org/0000-0003-4395-523X https://orcid.org/0000-0003-3965-0740 https://orcid.org/0000-0003-1085-7692 https://orcid.org/0000-0001-8323-2779 https://orcid.org/0000-0003-3570-8917 https://orcid.org/0000-0003-3530-5819 en_US http://dx.doi.org/10.1038/micronano.2015.37 Microsystems & Nanoengineering Creative Commons Attribution 4.0 International License http://creativecommons.org/licenses/by/4.0/ application/pdf Springer Nature Nature |
| spellingShingle | Toner, Mehmet Yost, Allison Lynne Shahsavari, Setareh Bradwell, Grinia M. Polak, Roberta Fachin, Fabio Cohen, Robert E McKinley, Gareth H Rubner, Michael F Wardle, Brian L Layer-by-layer functionalized nanotube arrays: A versatile microfluidic platform for biodetection |
| title | Layer-by-layer functionalized nanotube arrays: A versatile microfluidic platform for biodetection |
| title_full | Layer-by-layer functionalized nanotube arrays: A versatile microfluidic platform for biodetection |
| title_fullStr | Layer-by-layer functionalized nanotube arrays: A versatile microfluidic platform for biodetection |
| title_full_unstemmed | Layer-by-layer functionalized nanotube arrays: A versatile microfluidic platform for biodetection |
| title_short | Layer-by-layer functionalized nanotube arrays: A versatile microfluidic platform for biodetection |
| title_sort | layer by layer functionalized nanotube arrays a versatile microfluidic platform for biodetection |
| url | http://hdl.handle.net/1721.1/111955 https://orcid.org/0000-0003-2399-4397 https://orcid.org/0000-0003-4395-523X https://orcid.org/0000-0003-3965-0740 https://orcid.org/0000-0003-1085-7692 https://orcid.org/0000-0001-8323-2779 https://orcid.org/0000-0003-3570-8917 https://orcid.org/0000-0003-3530-5819 |
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