Microbial community assembly and evolution in subseafloor sediment
Bacterial and archaeal communities inhabiting the subsurface seabed live under strong energy limitation and have growth rates that are orders of magnitude slower than laboratory-grown cultures. It is not understood how subsurface microbial communities are assembled and whether populations undergo ad...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
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National Academy of Sciences (U.S.)
2017
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| Online Access: | http://hdl.handle.net/1721.1/112220 https://orcid.org/0000-0001-9296-3733 |
| _version_ | 1811086718782144512 |
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| author | Starnawski, Piotr Bataillon, Thomas Ettema, Thijs J. G. Jochum, Lara M. Schreiber, Lars Chen, Xihan Lever, Mark A. Jørgensen, Bo B. Kjeldsen, Kasper U. Polz, Martin F Schramm, Andreas |
| author2 | Massachusetts Institute of Technology. Department of Civil and Environmental Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Civil and Environmental Engineering Starnawski, Piotr Bataillon, Thomas Ettema, Thijs J. G. Jochum, Lara M. Schreiber, Lars Chen, Xihan Lever, Mark A. Jørgensen, Bo B. Kjeldsen, Kasper U. Polz, Martin F Schramm, Andreas |
| author_sort | Starnawski, Piotr |
| collection | MIT |
| description | Bacterial and archaeal communities inhabiting the subsurface seabed live under strong energy limitation and have growth rates that are orders of magnitude slower than laboratory-grown cultures. It is not understood how subsurface microbial communities are assembled and whether populations undergo adaptive evolution or accumulate mutations as a result of impaired DNA repair under such energy-limited conditions. Here we use amplicon sequencing to explore changes of microbial communities during burial and isolation from the surface to the > 5,000-y-old subsurface of marine sediment and identify a small core set of mostly uncultured bacteria and archaea that is present throughout the sediment column. These persisting populations constitute a small fraction of the entire community at the surface but become predominant in the subsurface. We followed patterns of genome diversity with depth in four dominant lineages of the persisting populations by mapping metagenomic sequence reads onto single-cell genomes. Nucleotide sequence diversity was uniformly low and did not change with age and depth of the sediment. Likewise, therewas no detectable change inmutation rates and efficacy of selection. Our results indicate that subsurface microbial communities predominantly assemble by selective survival of taxa able to persist under extreme energy limitation. |
| first_indexed | 2024-09-23T13:32:31Z |
| format | Article |
| id | mit-1721.1/112220 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T13:32:31Z |
| publishDate | 2017 |
| publisher | National Academy of Sciences (U.S.) |
| record_format | dspace |
| spelling | mit-1721.1/1122202022-10-01T15:39:53Z Microbial community assembly and evolution in subseafloor sediment Starnawski, Piotr Bataillon, Thomas Ettema, Thijs J. G. Jochum, Lara M. Schreiber, Lars Chen, Xihan Lever, Mark A. Jørgensen, Bo B. Kjeldsen, Kasper U. Polz, Martin F Schramm, Andreas Massachusetts Institute of Technology. Department of Civil and Environmental Engineering Parsons Laboratory for Environmental Science and Engineering (Massachusetts Institute of Technology) Polz, Martin F Schramm, Andreas Bacterial and archaeal communities inhabiting the subsurface seabed live under strong energy limitation and have growth rates that are orders of magnitude slower than laboratory-grown cultures. It is not understood how subsurface microbial communities are assembled and whether populations undergo adaptive evolution or accumulate mutations as a result of impaired DNA repair under such energy-limited conditions. Here we use amplicon sequencing to explore changes of microbial communities during burial and isolation from the surface to the > 5,000-y-old subsurface of marine sediment and identify a small core set of mostly uncultured bacteria and archaea that is present throughout the sediment column. These persisting populations constitute a small fraction of the entire community at the surface but become predominant in the subsurface. We followed patterns of genome diversity with depth in four dominant lineages of the persisting populations by mapping metagenomic sequence reads onto single-cell genomes. Nucleotide sequence diversity was uniformly low and did not change with age and depth of the sediment. Likewise, therewas no detectable change inmutation rates and efficacy of selection. Our results indicate that subsurface microbial communities predominantly assemble by selective survival of taxa able to persist under extreme energy limitation. 2017-11-17T15:12:29Z 2017-11-17T15:12:29Z 2017-02 2016-08 2017-10-30T19:31:24Z Article http://purl.org/eprint/type/JournalArticle 0027-8424 1091-6490 http://hdl.handle.net/1721.1/112220 Starnawski, Piotr et al. “Microbial Community Assembly and Evolution in Subseafloor Sediment.” Proceedings of the National Academy of Sciences 114, 11 (February 2017): 2940–2945 © 2017 National Academy of Sciences https://orcid.org/0000-0001-9296-3733 http://dx.doi.org/10.1073/PNAS.1614190114 Proceedings of the National Academy of Sciences Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf National Academy of Sciences (U.S.) PNAS |
| spellingShingle | Starnawski, Piotr Bataillon, Thomas Ettema, Thijs J. G. Jochum, Lara M. Schreiber, Lars Chen, Xihan Lever, Mark A. Jørgensen, Bo B. Kjeldsen, Kasper U. Polz, Martin F Schramm, Andreas Microbial community assembly and evolution in subseafloor sediment |
| title | Microbial community assembly and evolution in subseafloor sediment |
| title_full | Microbial community assembly and evolution in subseafloor sediment |
| title_fullStr | Microbial community assembly and evolution in subseafloor sediment |
| title_full_unstemmed | Microbial community assembly and evolution in subseafloor sediment |
| title_short | Microbial community assembly and evolution in subseafloor sediment |
| title_sort | microbial community assembly and evolution in subseafloor sediment |
| url | http://hdl.handle.net/1721.1/112220 https://orcid.org/0000-0001-9296-3733 |
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