Modeling two-photon calcium fluorescence of episodic V1 recordings using multifrequency analysis
The use of two-photon microscopy allows for imaging of deep neural tissue in vivo. This paper examines frequency-based analysis to two-photon calcium fluorescence images with the goal of deriving smooth tuning curves. We present a multifrequency analysis approach for improved extraction of calcium r...
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| Format: | Article |
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Institute of Electrical and Electronics Engineers (IEEE)
2017
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| Online Access: | http://hdl.handle.net/1721.1/112250 https://orcid.org/0000-0002-7260-7560 https://orcid.org/0000-0003-2442-5671 https://orcid.org/0000-0003-2668-7819 |
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| author | Zheng, H. W. Malik, Wasim Qamar Runyan, Caroline Anne Sur, Mriganka Brown, Emery Neal |
| author2 | Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences |
| author_facet | Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Zheng, H. W. Malik, Wasim Qamar Runyan, Caroline Anne Sur, Mriganka Brown, Emery Neal |
| author_sort | Zheng, H. W. |
| collection | MIT |
| description | The use of two-photon microscopy allows for imaging of deep neural tissue in vivo. This paper examines frequency-based analysis to two-photon calcium fluorescence images with the goal of deriving smooth tuning curves. We present a multifrequency analysis approach for improved extraction of calcium responses in episodic stimulation experiments, that is, when the stimulus is applied for a number of frames, then turned off for the next few frames, and so on. Episodic orientation stimulus was applied while recording from the primary visual cortex of an anesthetized mouse. The multifrequency model demonstrated improved tuning curve descriptions of the neurons. It also offers perspective regarding the characteristics of calcium fluorescence imaging of the brain. |
| first_indexed | 2024-09-23T09:35:01Z |
| format | Article |
| id | mit-1721.1/112250 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T09:35:01Z |
| publishDate | 2017 |
| publisher | Institute of Electrical and Electronics Engineers (IEEE) |
| record_format | dspace |
| spelling | mit-1721.1/1122502022-09-26T12:28:18Z Modeling two-photon calcium fluorescence of episodic V1 recordings using multifrequency analysis Zheng, H. W. Malik, Wasim Qamar Runyan, Caroline Anne Sur, Mriganka Brown, Emery Neal Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Picower Institute for Learning and Memory Malik, Wasim Qamar Runyan, Caroline Anne Sur, Mriganka Brown, Emery Neal The use of two-photon microscopy allows for imaging of deep neural tissue in vivo. This paper examines frequency-based analysis to two-photon calcium fluorescence images with the goal of deriving smooth tuning curves. We present a multifrequency analysis approach for improved extraction of calcium responses in episodic stimulation experiments, that is, when the stimulus is applied for a number of frames, then turned off for the next few frames, and so on. Episodic orientation stimulus was applied while recording from the primary visual cortex of an anesthetized mouse. The multifrequency model demonstrated improved tuning curve descriptions of the neurons. It also offers perspective regarding the characteristics of calcium fluorescence imaging of the brain. National Institutes of Health (U.S.) (Grant DP1-OD003646) National Institutes of Health (U.S.) (Grant R01-EB006385) National Institutes of Health (U.S.) (Grant EY07023) National Institutes of Health (U.S.) (Grant EY017098) 2017-11-20T18:50:14Z 2017-11-20T18:50:14Z 2011-12 2011-08 2017-11-01T17:01:57Z Article http://purl.org/eprint/type/ConferencePaper 978-1-4577-1589-1 978-1-4244-4121-1 978-1-4244-4122-8 1557-170X 1094-687X 1558-4615 http://hdl.handle.net/1721.1/112250 Zheng, H. W. et al. “Modeling Two-Photon Calcium Fluorescence of Episodic V1 Recordings Using Multifrequency Analysis.” 2011 Annual International Conference of the IEEE Engineering in Medicine and Biology Society, August 30 - September 3 2011, Boston, Massachusetts, USA, Institute of Electrical and Electronics Engineers (IEEE), December 2011 © 2011 Institute of Electrical and Electronics Engineers (IEEE) https://orcid.org/0000-0002-7260-7560 https://orcid.org/0000-0003-2442-5671 https://orcid.org/0000-0003-2668-7819 http://dx.doi.org/10.1109/IEMBS.2011.6090826 2011 Annual International Conference of the IEEE Engineering in Medicine and Biology Society Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Institute of Electrical and Electronics Engineers (IEEE) PMC |
| spellingShingle | Zheng, H. W. Malik, Wasim Qamar Runyan, Caroline Anne Sur, Mriganka Brown, Emery Neal Modeling two-photon calcium fluorescence of episodic V1 recordings using multifrequency analysis |
| title | Modeling two-photon calcium fluorescence of episodic V1 recordings using multifrequency analysis |
| title_full | Modeling two-photon calcium fluorescence of episodic V1 recordings using multifrequency analysis |
| title_fullStr | Modeling two-photon calcium fluorescence of episodic V1 recordings using multifrequency analysis |
| title_full_unstemmed | Modeling two-photon calcium fluorescence of episodic V1 recordings using multifrequency analysis |
| title_short | Modeling two-photon calcium fluorescence of episodic V1 recordings using multifrequency analysis |
| title_sort | modeling two photon calcium fluorescence of episodic v1 recordings using multifrequency analysis |
| url | http://hdl.handle.net/1721.1/112250 https://orcid.org/0000-0002-7260-7560 https://orcid.org/0000-0003-2442-5671 https://orcid.org/0000-0003-2668-7819 |
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