Design and development of components of a modular bioreactor
Thesis: S.M., Massachusetts Institute of Technology, Department of Mechanical Engineering, 2017.
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| Other Authors: | |
| Format: | Thesis |
| Language: | eng |
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Massachusetts Institute of Technology
2017
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| Online Access: | http://hdl.handle.net/1721.1/112524 |
| _version_ | 1826217139647807488 |
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| author | Mascarenhas, Craig Anthony |
| author2 | J. Christopher Love. |
| author_facet | J. Christopher Love. Mascarenhas, Craig Anthony |
| author_sort | Mascarenhas, Craig Anthony |
| collection | MIT |
| description | Thesis: S.M., Massachusetts Institute of Technology, Department of Mechanical Engineering, 2017. |
| first_indexed | 2024-09-23T16:58:39Z |
| format | Thesis |
| id | mit-1721.1/112524 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T16:58:39Z |
| publishDate | 2017 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/1125242019-04-12T22:49:21Z Design and development of components of a modular bioreactor Mascarenhas, Craig Anthony J. Christopher Love. Massachusetts Institute of Technology. Department of Mechanical Engineering. Massachusetts Institute of Technology. Department of Mechanical Engineering. Mechanical Engineering. Thesis: S.M., Massachusetts Institute of Technology, Department of Mechanical Engineering, 2017. Page 206 blank. Cataloged from PDF version of thesis. Includes bibliographical references (pages 196-205). Biologic drug manufacturing is traditionally conducted in large-scale, industrial bioreactors. The emergence of interest in disposable, bench-top bioreactors as a viable alternative is due to potential advantages such as lower contamination risk, time and cost savings, and ease of handling. The challenges associated with disposable, bench-top bioreactors include poor mixing, limited oxygen transfer, and a scarcity of non-invasive sensors for process control. This thesis investigates multiple aspects of a disposable, perfusion-capable bioreactor, in order to facilitate an optimal design. In order to determine an impeller configuration that improves the mixing and mass transfer characteristics of a i-liter bioreactor, Computational Fluid Dynamics (CFD) was used. The potential benefits of switching to a dual-Marine impeller system was revealed, which was then validated during fermentation experiments. Further predictions of a merging flow pattern in the i-liter vessel was consistent with the literature based on the impeller spacing. A scaled-up 5-liter vessel was designed with Rushton impellers spaced so as to create a parallel flow pattern, which was later successfully predicted in the CFD simulations. Flow patterns were analyzed at various locations in both vessels to aid future design iterations. Monitoring of process parameters, including liquid level, is important for automated control in bioreactors. Three novel, non-invasive, optical liquid level sensing methods were conceptualized, prototyped, and successfully tested. These solutions relied on self-developed image processing algorithms. Additionally, a magnetic liquid level sensor was also developed and tested that relied on a magnetic float and a series of reed switches. In order to increase the perfusion membrane surface area and reduce complexity, the switch to a hollow-fiber harvest probe was examined. CFD studies guided design iterations by modeling the flow around the probe, giving insight into the stagnation properties and shear forces acting on the fibers. Additionally, experimental testing of the new harvest probe revealed its successful functionality and viability in the bioreactor. by Craig Anthony Mascarenhas. S.M. 2017-12-05T19:16:43Z 2017-12-05T19:16:43Z 2017 2017 Thesis http://hdl.handle.net/1721.1/112524 1012939123 eng MIT theses are protected by copyright. They may be viewed, downloaded, or printed from this source but further reproduction or distribution in any format is prohibited without written permission. http://dspace.mit.edu/handle/1721.1/7582 206 pages application/pdf Massachusetts Institute of Technology |
| spellingShingle | Mechanical Engineering. Mascarenhas, Craig Anthony Design and development of components of a modular bioreactor |
| title | Design and development of components of a modular bioreactor |
| title_full | Design and development of components of a modular bioreactor |
| title_fullStr | Design and development of components of a modular bioreactor |
| title_full_unstemmed | Design and development of components of a modular bioreactor |
| title_short | Design and development of components of a modular bioreactor |
| title_sort | design and development of components of a modular bioreactor |
| topic | Mechanical Engineering. |
| url | http://hdl.handle.net/1721.1/112524 |
| work_keys_str_mv | AT mascarenhascraiganthony designanddevelopmentofcomponentsofamodularbioreactor |