Structure and Engineering of Francisella novicida Cas9

Structure and Engineering of Francisella novicida Cas9

Summary The RNA-guided endonuclease Cas9 cleaves double-stranded DNA targets complementary to the guide RNA and has been applied to programmable genome editing. Cas9-mediated cleavage requires a protospacer adjacent motif (PAM) juxtaposed with the DNA target sequence, thus constricting the range of...

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Bibliographic Details
Main Authors:	Hirano, Hisato, Horii, Takuro, Kimura, Mika, Nakane, Takanori, Ishitani, Ryuichiro, Hatada, Izuho, Nishimasu, Hiroshi, Nureki, Osamu, Gootenberg, Jonathan S, Abudayyeh, Omar Osama, Hsu, Patrick, Zhang, Feng
Other Authors:	Massachusetts Institute of Technology. Department of Biological Engineering
Format:	Article
Published:	Elsevier 2017
Online Access:	http://hdl.handle.net/1721.1/112719 https://orcid.org/0000-0002-7979-3220 https://orcid.org/0000-0003-2782-2509

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