Hydrogel microparticles for biosensing
Due to their hydrophilic, biocompatible, and highly tunable nature, hydrogel materials have attracted strong interest in the recent years for numerous biotechnological applications. In particular, their solution-like environment and non-fouling nature in complex biological samples render hydrogels a...
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| Format: | Article |
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Elsevier BV
2017
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| Online Access: | http://hdl.handle.net/1721.1/112942 https://orcid.org/0000-0002-9781-0135 |
| _version_ | 1811083595958190080 |
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| author | Hill, W. Adam Le Goff, Gaelle Srinivas, Rathi L. Doyle, Patrick S |
| author2 | Massachusetts Institute of Technology. Department of Chemical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Chemical Engineering Hill, W. Adam Le Goff, Gaelle Srinivas, Rathi L. Doyle, Patrick S |
| author_sort | Hill, W. Adam |
| collection | MIT |
| description | Due to their hydrophilic, biocompatible, and highly tunable nature, hydrogel materials have attracted strong interest in the recent years for numerous biotechnological applications. In particular, their solution-like environment and non-fouling nature in complex biological samples render hydrogels as ideal substrates for biosensing applications. Hydrogel coatings, and later, gel dot surface microarrays, were successfully used in sensitive nucleic acid assays and immunoassays. More recently, new microfabrication techniques for synthesizing encoded particles from hydrogel materials have enabled the development of hydrogel-based suspension arrays. Lithography processes and droplet-based microfluidic techniques enable generation of libraries of particles with unique spectral or graphical codes, for multiplexed sensing in biological samples. In this review, we discuss the key questions arising when designing hydrogel particles dedicated to biosensing. How can the hydrogel material be engineered in order to tune its properties and immobilize bioprobes inside? What are the strategies to fabricate and encode gel particles, and how can particles be processed and decoded after the assay? Finally, we review the bioassays reported so far in the literature that have used hydrogel particle arrays and give an outlook of further developments of the field. Keywords: Hydrogel; Biosensor; Microparticle; Multiplex assay |
| first_indexed | 2024-09-23T12:35:34Z |
| format | Article |
| id | mit-1721.1/112942 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T12:35:34Z |
| publishDate | 2017 |
| publisher | Elsevier BV |
| record_format | dspace |
| spelling | mit-1721.1/1129422022-09-28T08:52:45Z Hydrogel microparticles for biosensing Hill, W. Adam Le Goff, Gaelle Srinivas, Rathi L. Doyle, Patrick S Massachusetts Institute of Technology. Department of Chemical Engineering Le Goff, Gaelle Srinivas, Rathi L. Doyle, Patrick S Due to their hydrophilic, biocompatible, and highly tunable nature, hydrogel materials have attracted strong interest in the recent years for numerous biotechnological applications. In particular, their solution-like environment and non-fouling nature in complex biological samples render hydrogels as ideal substrates for biosensing applications. Hydrogel coatings, and later, gel dot surface microarrays, were successfully used in sensitive nucleic acid assays and immunoassays. More recently, new microfabrication techniques for synthesizing encoded particles from hydrogel materials have enabled the development of hydrogel-based suspension arrays. Lithography processes and droplet-based microfluidic techniques enable generation of libraries of particles with unique spectral or graphical codes, for multiplexed sensing in biological samples. In this review, we discuss the key questions arising when designing hydrogel particles dedicated to biosensing. How can the hydrogel material be engineered in order to tune its properties and immobilize bioprobes inside? What are the strategies to fabricate and encode gel particles, and how can particles be processed and decoded after the assay? Finally, we review the bioassays reported so far in the literature that have used hydrogel particle arrays and give an outlook of further developments of the field. Keywords: Hydrogel; Biosensor; Microparticle; Multiplex assay Novartis Institutes of Biomedical Research (Presidential Fellowship) Novartis Institutes of Biomedical Research (Education Office) National Cancer Institute (U.S.) (Grant 5R21CA177393-02) National Science Foundation (U.S.) (Grant CMMI-1120724) Institute for Collaborative Biotechnologies (Grant W911NF-09-0001) United States. Army Research Office 2017-12-22T20:14:22Z 2017-12-22T20:14:22Z 2015-02 2014-12 2017-12-22T15:07:09Z Article http://purl.org/eprint/type/JournalArticle 0014-3057 http://hdl.handle.net/1721.1/112942 Le Goff, Gaelle C., et al. “Hydrogel Microparticles for Biosensing.” European Polymer Journal, vol. 72, Nov. 2015, pp. 386–412. https://orcid.org/0000-0002-9781-0135 http://dx.doi.org/10.1016/J.EURPOLYMJ.2015.02.022 European Polymer Journal Creative Commons Attribution-NonCommercial-NoDerivs License http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Elsevier BV PMC |
| spellingShingle | Hill, W. Adam Le Goff, Gaelle Srinivas, Rathi L. Doyle, Patrick S Hydrogel microparticles for biosensing |
| title | Hydrogel microparticles for biosensing |
| title_full | Hydrogel microparticles for biosensing |
| title_fullStr | Hydrogel microparticles for biosensing |
| title_full_unstemmed | Hydrogel microparticles for biosensing |
| title_short | Hydrogel microparticles for biosensing |
| title_sort | hydrogel microparticles for biosensing |
| url | http://hdl.handle.net/1721.1/112942 https://orcid.org/0000-0002-9781-0135 |
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