Optical visualization and quantification of enzyme activity using dynamic droplet lenses
In this paper, we describe an approach to measuring enzyme activity based on the reconfiguration of complex emulsions. Changes in the morphology of these complex emulsions, driven by enzyme-responsive surfactants, modulate the transmission of light through a sample. Through this method we demonstrat...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Article |
| Published: |
National Academy of Sciences (U.S.)
2018
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| Online Access: | http://hdl.handle.net/1721.1/113238 https://orcid.org/0000-0002-3287-3602 https://orcid.org/0000-0002-4449-9566 |
| Summary: | In this paper, we describe an approach to measuring enzyme activity based on the reconfiguration of complex emulsions. Changes in the morphology of these complex emulsions, driven by enzyme-responsive surfactants, modulate the transmission of light through a sample. Through this method we demonstrate how simple photodetector measurements may be used to monitor enzyme kinetics. This approach is validated by quantitative measurements of enzyme activity for three different classes of enzymes (amylase, lipase, and sulfatase), relying on two distinct mechanisms for coupling droplet morphology to enzyme activity (host–guest interactions with uncaging and molecular cleavage). |
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