Optical visualization and quantification of enzyme activity using dynamic droplet lenses
In this paper, we describe an approach to measuring enzyme activity based on the reconfiguration of complex emulsions. Changes in the morphology of these complex emulsions, driven by enzyme-responsive surfactants, modulate the transmission of light through a sample. Through this method we demonstrat...
| Main Authors: | , , , , |
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| Format: | Article |
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National Academy of Sciences (U.S.)
2018
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| Online Access: | http://hdl.handle.net/1721.1/113238 https://orcid.org/0000-0002-3287-3602 https://orcid.org/0000-0002-4449-9566 |
| _version_ | 1826202846578606080 |
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| author | Zarzar, Lauren D. Kalow, Julia Ann He, Xinping Walish, Joseph John Swager, Timothy M |
| author2 | Massachusetts Institute of Technology. Institute for Soldier Nanotechnologies |
| author_facet | Massachusetts Institute of Technology. Institute for Soldier Nanotechnologies Zarzar, Lauren D. Kalow, Julia Ann He, Xinping Walish, Joseph John Swager, Timothy M |
| author_sort | Zarzar, Lauren D. |
| collection | MIT |
| description | In this paper, we describe an approach to measuring enzyme activity based on the reconfiguration of complex emulsions. Changes in the morphology of these complex emulsions, driven by enzyme-responsive surfactants, modulate the transmission of light through a sample. Through this method we demonstrate how simple photodetector measurements may be used to monitor enzyme kinetics. This approach is validated by quantitative measurements of enzyme activity for three different classes of enzymes (amylase, lipase, and sulfatase), relying on two distinct mechanisms for coupling droplet morphology to enzyme activity (host–guest interactions with uncaging and molecular cleavage). |
| first_indexed | 2024-09-23T12:20:30Z |
| format | Article |
| id | mit-1721.1/113238 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T12:20:30Z |
| publishDate | 2018 |
| publisher | National Academy of Sciences (U.S.) |
| record_format | dspace |
| spelling | mit-1721.1/1132382019-05-17T07:57:20Z Optical visualization and quantification of enzyme activity using dynamic droplet lenses Zarzar, Lauren D. Kalow, Julia Ann He, Xinping Walish, Joseph John Swager, Timothy M Massachusetts Institute of Technology. Institute for Soldier Nanotechnologies Massachusetts Institute of Technology. Department of Chemistry Zarzar, Lauren D. Kalow, Julia Ann He, Xinping Walish, Joseph John Swager, Timothy M In this paper, we describe an approach to measuring enzyme activity based on the reconfiguration of complex emulsions. Changes in the morphology of these complex emulsions, driven by enzyme-responsive surfactants, modulate the transmission of light through a sample. Through this method we demonstrate how simple photodetector measurements may be used to monitor enzyme kinetics. This approach is validated by quantitative measurements of enzyme activity for three different classes of enzymes (amylase, lipase, and sulfatase), relying on two distinct mechanisms for coupling droplet morphology to enzyme activity (host–guest interactions with uncaging and molecular cleavage). Massachusetts Institute of Technology. Institute for Soldier Nanotechnologies National Institutes of Health (U.S.). Ruth L. Kirschstein National Research Service Award (Award GM106550) Tianjin University (Internship Program) 2018-01-19T18:34:51Z 2018-01-19T18:34:51Z 2017-03 2016-11 2018-01-17T12:02:07Z Article http://purl.org/eprint/type/JournalArticle 0027-8424 1091-6490 http://hdl.handle.net/1721.1/113238 Zarzar, Lauren D., et al. “Optical Visualization and Quantification of Enzyme Activity Using Dynamic Droplet Lenses.” Proceedings of the National Academy of Sciences, vol. 114, no. 15, Apr. 2017, pp. 3821–25. © 2018 National Academy of Sciences PUBLISHER_POLICY https://orcid.org/0000-0002-3287-3602 https://orcid.org/0000-0002-4449-9566 http://dx.doi.org/10.1073/PNAS.1618807114 Proceedings of the National Academy of Sciences Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/octet-stream National Academy of Sciences (U.S.) PNAS |
| spellingShingle | Zarzar, Lauren D. Kalow, Julia Ann He, Xinping Walish, Joseph John Swager, Timothy M Optical visualization and quantification of enzyme activity using dynamic droplet lenses |
| title | Optical visualization and quantification of enzyme activity using dynamic droplet lenses |
| title_full | Optical visualization and quantification of enzyme activity using dynamic droplet lenses |
| title_fullStr | Optical visualization and quantification of enzyme activity using dynamic droplet lenses |
| title_full_unstemmed | Optical visualization and quantification of enzyme activity using dynamic droplet lenses |
| title_short | Optical visualization and quantification of enzyme activity using dynamic droplet lenses |
| title_sort | optical visualization and quantification of enzyme activity using dynamic droplet lenses |
| url | http://hdl.handle.net/1721.1/113238 https://orcid.org/0000-0002-3287-3602 https://orcid.org/0000-0002-4449-9566 |
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