Seq-Well: portable, low-cost RNA sequencing of single cells at high throughput
Single-cell rna-seq can precisely resolve cellular states, but applying this method to low-input samples is challenging. here, we present seq-Well, a portable, low-cost platform for massively parallel single-cell rna-seq. barcoded mrna capture beads and single cells are sealed in an array of subnano...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
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Springer Nature
2018
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| Online Access: | http://hdl.handle.net/1721.1/113430 https://orcid.org/0000-0001-9376-164X https://orcid.org/0000-0001-8291-8672 |
| _version_ | 1811069288871624704 |
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| author | Bryson, Bryan D Butler, Andrew Satija, Rahul Fortune, Sarah Love, J Christopher Gierahn, Todd Michael Wadsworth, Marc Havens Hughes, Travis K. Shalek, Alexander K |
| author2 | Massachusetts Institute of Technology. Institute for Medical Engineering & Science |
| author_facet | Massachusetts Institute of Technology. Institute for Medical Engineering & Science Bryson, Bryan D Butler, Andrew Satija, Rahul Fortune, Sarah Love, J Christopher Gierahn, Todd Michael Wadsworth, Marc Havens Hughes, Travis K. Shalek, Alexander K |
| author_sort | Bryson, Bryan D |
| collection | MIT |
| description | Single-cell rna-seq can precisely resolve cellular states, but applying this method to low-input samples is challenging. here, we present seq-Well, a portable, low-cost platform for massively parallel single-cell rna-seq. barcoded mrna capture beads and single cells are sealed in an array of subnanoliter wells using a semipermeable membrane, enabling effcient cell lysis and transcript capture. We use seq-Well to profle thousands of primary human macrophages exposed to Mycobacterium tuberculosis. |
| first_indexed | 2024-09-23T08:08:43Z |
| format | Article |
| id | mit-1721.1/113430 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T08:08:43Z |
| publishDate | 2018 |
| publisher | Springer Nature |
| record_format | dspace |
| spelling | mit-1721.1/1134302022-09-30T07:53:53Z Seq-Well: portable, low-cost RNA sequencing of single cells at high throughput Bryson, Bryan D Butler, Andrew Satija, Rahul Fortune, Sarah Love, J Christopher Gierahn, Todd Michael Wadsworth, Marc Havens Hughes, Travis K. Shalek, Alexander K Massachusetts Institute of Technology. Institute for Medical Engineering & Science Massachusetts Institute of Technology. Department of Chemistry Koch Institute for Integrative Cancer Research at MIT Gierahn, Todd Michael Wadsworth, Marc Havens Hughes, Travis K. Shalek, Alexander K Single-cell rna-seq can precisely resolve cellular states, but applying this method to low-input samples is challenging. here, we present seq-Well, a portable, low-cost platform for massively parallel single-cell rna-seq. barcoded mrna capture beads and single cells are sealed in an array of subnanoliter wells using a semipermeable membrane, enabling effcient cell lysis and transcript capture. We use seq-Well to profle thousands of primary human macrophages exposed to Mycobacterium tuberculosis. National Institutes of Health (U.S.) (Award DP2 OD020839) National Institutes of Health (U.S.) (Grant U24 AI118762) National Institutes of Health (U.S.) (Grant P50 HG006193) Bill & Melinda Gates Foundation (Grant 03629000189) 2018-02-06T15:05:16Z 2018-02-06T15:05:16Z 2017-02 2016-07 2018-02-05T17:26:56Z Article http://purl.org/eprint/type/JournalArticle 1548-7091 1548-7105 http://hdl.handle.net/1721.1/113430 Gierahn, Todd M et al. “Seq-Well: Portable, Low-Cost RNA Sequencing of Single Cells at High Throughput.” Nature Methods 14, 4 (February 2017): 395–398 © 2017 KSBMB https://orcid.org/0000-0001-9376-164X https://orcid.org/0000-0001-8291-8672 http://dx.doi.org/10.1038/NMETH.4179 Nature Methods Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Springer Nature PMC |
| spellingShingle | Bryson, Bryan D Butler, Andrew Satija, Rahul Fortune, Sarah Love, J Christopher Gierahn, Todd Michael Wadsworth, Marc Havens Hughes, Travis K. Shalek, Alexander K Seq-Well: portable, low-cost RNA sequencing of single cells at high throughput |
| title | Seq-Well: portable, low-cost RNA sequencing of single cells at high throughput |
| title_full | Seq-Well: portable, low-cost RNA sequencing of single cells at high throughput |
| title_fullStr | Seq-Well: portable, low-cost RNA sequencing of single cells at high throughput |
| title_full_unstemmed | Seq-Well: portable, low-cost RNA sequencing of single cells at high throughput |
| title_short | Seq-Well: portable, low-cost RNA sequencing of single cells at high throughput |
| title_sort | seq well portable low cost rna sequencing of single cells at high throughput |
| url | http://hdl.handle.net/1721.1/113430 https://orcid.org/0000-0001-9376-164X https://orcid.org/0000-0001-8291-8672 |
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