Two-photon imaging in mice shows striosomes and matrix have overlapping but differential reinforcement-related responses
Striosomes were discovered several decades ago as neurochemically identified zones in the striatum, yet technical hurdles have hampered the study of the functions of these striatal compartments. Here we used 2-photon calcium imaging in neuronal birthdate-labeled Mash1- CreER;Ai14 mice to image simul...
| Main Authors: | , , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Published: |
eLife Sciences Publications, Ltd
2018
|
| Online Access: | http://hdl.handle.net/1721.1/113562 https://orcid.org/0000-0002-0930-580X https://orcid.org/0000-0002-6814-9966 https://orcid.org/0000-0003-2442-5671 https://orcid.org/0000-0002-4326-7720 |
| _version_ | 1826200303199846400 |
|---|---|
| author | Bloem, Bernard Huda, Rafiq Sur, Mriganka Graybiel, Ann M |
| author2 | Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences |
| author_facet | Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Bloem, Bernard Huda, Rafiq Sur, Mriganka Graybiel, Ann M |
| author_sort | Bloem, Bernard |
| collection | MIT |
| description | Striosomes were discovered several decades ago as neurochemically identified zones in the striatum, yet technical hurdles have hampered the study of the functions of these striatal compartments. Here we used 2-photon calcium imaging in neuronal birthdate-labeled Mash1- CreER;Ai14 mice to image simultaneously the activity of striosomal and matrix neurons as mice performed an auditory conditioning task. With this method, we identified circumscribed zones of tdTomato-labeled neuropil that correspond to striosomes as verified immunohistochemically. Neurons in both striosomes and matrix responded to reward-predicting cues and were active during or after consummatory licking. However, we found quantitative differences in response strength: striosomal neurons fired more to reward-predicting cues and encoded more information about expected outcome as mice learned the task, whereas matrix neurons were more strongly modulated by recent reward history. These findings open the possibility of harnessing in vivo imaging to determine the contributions of striosomes and matrix to striatal circuit function. |
| first_indexed | 2024-09-23T11:34:24Z |
| format | Article |
| id | mit-1721.1/113562 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T11:34:24Z |
| publishDate | 2018 |
| publisher | eLife Sciences Publications, Ltd |
| record_format | dspace |
| spelling | mit-1721.1/1135622022-10-01T04:31:57Z Two-photon imaging in mice shows striosomes and matrix have overlapping but differential reinforcement-related responses Bloem, Bernard Huda, Rafiq Sur, Mriganka Graybiel, Ann M Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences McGovern Institute for Brain Research at MIT Picower Institute for Learning and Memory Bloem, Bernard Huda, Rafiq Sur, Mriganka Graybiel, Ann M Striosomes were discovered several decades ago as neurochemically identified zones in the striatum, yet technical hurdles have hampered the study of the functions of these striatal compartments. Here we used 2-photon calcium imaging in neuronal birthdate-labeled Mash1- CreER;Ai14 mice to image simultaneously the activity of striosomal and matrix neurons as mice performed an auditory conditioning task. With this method, we identified circumscribed zones of tdTomato-labeled neuropil that correspond to striosomes as verified immunohistochemically. Neurons in both striosomes and matrix responded to reward-predicting cues and were active during or after consummatory licking. However, we found quantitative differences in response strength: striosomal neurons fired more to reward-predicting cues and encoded more information about expected outcome as mice learned the task, whereas matrix neurons were more strongly modulated by recent reward history. These findings open the possibility of harnessing in vivo imaging to determine the contributions of striosomes and matrix to striatal circuit function. National Institute of Neurological Diseases and Stroke (U.S.) (Grant U01 NS090473) National Eye Institute (Grant R01 EY007023) National Science Foundation (U.S.) (Grant EF1451125) National Eye Institute (Grant F32 EY024857) National Institute of Mental Health (U.S.) (Grant K99 MH112855) 2018-02-09T17:24:20Z 2018-02-09T17:24:20Z 2017-12 2017-09 2018-02-02T19:10:17Z Article http://purl.org/eprint/type/JournalArticle 2050-084X http://hdl.handle.net/1721.1/113562 Bloem, Bernard et al. “Two-Photon Imaging in Mice Shows Striosomes and Matrix Have Overlapping but Differential Reinforcement-Related Responses.” eLife 2017, 6 (December 2017): e32353 © Bloem et al https://orcid.org/0000-0002-0930-580X https://orcid.org/0000-0002-6814-9966 https://orcid.org/0000-0003-2442-5671 https://orcid.org/0000-0002-4326-7720 http://dx.doi.org/10.7554/eLife.32353 eLife Creative Commons Attribution 4.0 International License https://creativecommons.org/licenses/by/4.0/ application/pdf eLife Sciences Publications, Ltd eLife |
| spellingShingle | Bloem, Bernard Huda, Rafiq Sur, Mriganka Graybiel, Ann M Two-photon imaging in mice shows striosomes and matrix have overlapping but differential reinforcement-related responses |
| title | Two-photon imaging in mice shows striosomes and matrix have overlapping but differential reinforcement-related responses |
| title_full | Two-photon imaging in mice shows striosomes and matrix have overlapping but differential reinforcement-related responses |
| title_fullStr | Two-photon imaging in mice shows striosomes and matrix have overlapping but differential reinforcement-related responses |
| title_full_unstemmed | Two-photon imaging in mice shows striosomes and matrix have overlapping but differential reinforcement-related responses |
| title_short | Two-photon imaging in mice shows striosomes and matrix have overlapping but differential reinforcement-related responses |
| title_sort | two photon imaging in mice shows striosomes and matrix have overlapping but differential reinforcement related responses |
| url | http://hdl.handle.net/1721.1/113562 https://orcid.org/0000-0002-0930-580X https://orcid.org/0000-0002-6814-9966 https://orcid.org/0000-0003-2442-5671 https://orcid.org/0000-0002-4326-7720 |
| work_keys_str_mv | AT bloembernard twophotonimaginginmiceshowsstriosomesandmatrixhaveoverlappingbutdifferentialreinforcementrelatedresponses AT hudarafiq twophotonimaginginmiceshowsstriosomesandmatrixhaveoverlappingbutdifferentialreinforcementrelatedresponses AT surmriganka twophotonimaginginmiceshowsstriosomesandmatrixhaveoverlappingbutdifferentialreinforcementrelatedresponses AT graybielannm twophotonimaginginmiceshowsstriosomesandmatrixhaveoverlappingbutdifferentialreinforcementrelatedresponses |