Actomyosin meshwork mechanosensing enables tissue shape to orient cell force
Sculpting organism shape requires that cells produce forces with proper directionality. Thus, it is critical to understand how cells orient the cytoskeleton to produce forces that deform tissues. During Drosophila gastrulation, actomyosin contraction in ventral cells generates a long, narrow epithel...
| Main Authors: | , , , , , |
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| Format: | Article |
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Nature Publishing Group
2018
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| Online Access: | http://hdl.handle.net/1721.1/113567 https://orcid.org/0000-0001-9434-0628 https://orcid.org/0000-0001-8060-2607 |
| _version_ | 1826205576611233792 |
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| author | Miller, Callie J. Ermentrout, Bard Davidson, Lance A. Chanet, Soline Vaishnav, Eeshit Dhaval Martin, Adam C |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Miller, Callie J. Ermentrout, Bard Davidson, Lance A. Chanet, Soline Vaishnav, Eeshit Dhaval Martin, Adam C |
| author_sort | Miller, Callie J. |
| collection | MIT |
| description | Sculpting organism shape requires that cells produce forces with proper directionality. Thus, it is critical to understand how cells orient the cytoskeleton to produce forces that deform tissues. During Drosophila gastrulation, actomyosin contraction in ventral cells generates a long, narrow epithelial furrow, termed the ventral furrow, in which actomyosin fibres and tension are directed along the length of the furrow. Using a combination of genetic and mechanical perturbations that alter tissue shape, we demonstrate that geometrical and mechanical constraints act as cues to orient the cytoskeleton and tension during ventral furrow formation. We developed an in silico model of two-dimensional actomyosin meshwork contraction, demonstrating that actomyosin meshworks exhibit an inherent force orienting mechanism in response to mechanical constraints. Together, our in vivo and in silico data provide a framework for understanding how cells orient force generation, establishing a role for geometrical and mechanical patterning of force production in tissues. |
| first_indexed | 2024-09-23T13:15:38Z |
| format | Article |
| id | mit-1721.1/113567 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T13:15:38Z |
| publishDate | 2018 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/1135672022-09-28T12:59:10Z Actomyosin meshwork mechanosensing enables tissue shape to orient cell force Miller, Callie J. Ermentrout, Bard Davidson, Lance A. Chanet, Soline Vaishnav, Eeshit Dhaval Martin, Adam C Massachusetts Institute of Technology. Department of Biology Chanet, Soline Vaishnav, Eeshit Dhaval Martin, Adam C Sculpting organism shape requires that cells produce forces with proper directionality. Thus, it is critical to understand how cells orient the cytoskeleton to produce forces that deform tissues. During Drosophila gastrulation, actomyosin contraction in ventral cells generates a long, narrow epithelial furrow, termed the ventral furrow, in which actomyosin fibres and tension are directed along the length of the furrow. Using a combination of genetic and mechanical perturbations that alter tissue shape, we demonstrate that geometrical and mechanical constraints act as cues to orient the cytoskeleton and tension during ventral furrow formation. We developed an in silico model of two-dimensional actomyosin meshwork contraction, demonstrating that actomyosin meshworks exhibit an inherent force orienting mechanism in response to mechanical constraints. Together, our in vivo and in silico data provide a framework for understanding how cells orient force generation, establishing a role for geometrical and mechanical patterning of force production in tissues. National Institute of General Medical Sciences (U.S.) (Grant R01GM105984) American Heart Association (Grant 14GRNT18880059) European Molecular Biology Organization (Grant ALTF 1082-2012) 2018-02-12T15:32:48Z 2018-02-12T15:32:48Z 2017-05 2016-09 2018-02-02T19:36:34Z Article http://purl.org/eprint/type/JournalArticle 2041-1723 http://hdl.handle.net/1721.1/113567 Chanet, Soline et al. “Actomyosin Meshwork Mechanosensing Enables Tissue Shape to Orient Cell Force.” Nature Communications 8 (May 2017): 15014 © 2017 The Author(s) https://orcid.org/0000-0001-9434-0628 https://orcid.org/0000-0001-8060-2607 http://dx.doi.org/10.1038/NCOMMS15014 Nature Communications Creative Commons Attribution 4.0 International License https://creativecommons.org/licenses/by/4.0/ application/pdf Nature Publishing Group Nature Communications |
| spellingShingle | Miller, Callie J. Ermentrout, Bard Davidson, Lance A. Chanet, Soline Vaishnav, Eeshit Dhaval Martin, Adam C Actomyosin meshwork mechanosensing enables tissue shape to orient cell force |
| title | Actomyosin meshwork mechanosensing enables tissue shape to orient cell force |
| title_full | Actomyosin meshwork mechanosensing enables tissue shape to orient cell force |
| title_fullStr | Actomyosin meshwork mechanosensing enables tissue shape to orient cell force |
| title_full_unstemmed | Actomyosin meshwork mechanosensing enables tissue shape to orient cell force |
| title_short | Actomyosin meshwork mechanosensing enables tissue shape to orient cell force |
| title_sort | actomyosin meshwork mechanosensing enables tissue shape to orient cell force |
| url | http://hdl.handle.net/1721.1/113567 https://orcid.org/0000-0001-9434-0628 https://orcid.org/0000-0001-8060-2607 |
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