Janus Emulsions for the Detection of Bacteria
Janus emulsion assays that rely on carbohydrate-lectin binding for the detection of Escherichia coli bacteria are described. Surfactants containing mannose are self-assembled at the surface of Janus droplets to produce particles with lectin binding sites. Janus droplets orient in a vertical directio...
| Main Authors: | , , , , |
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| Format: | Article |
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American Chemical Society (ACS)
2018
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| Online Access: | http://hdl.handle.net/1721.1/113582 https://orcid.org/0000-0001-8839-5091 |
| _version_ | 1826215460098539520 |
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| author | Kaplonek, Paulina Seeberger, Peter H. Zhang, Qifan Savagatrup, Suchol Swager, Timothy M |
| author2 | Massachusetts Institute of Technology. Institute for Soldier Nanotechnologies |
| author_facet | Massachusetts Institute of Technology. Institute for Soldier Nanotechnologies Kaplonek, Paulina Seeberger, Peter H. Zhang, Qifan Savagatrup, Suchol Swager, Timothy M |
| author_sort | Kaplonek, Paulina |
| collection | MIT |
| description | Janus emulsion assays that rely on carbohydrate-lectin binding for the detection of Escherichia coli bacteria are described. Surfactants containing mannose are self-assembled at the surface of Janus droplets to produce particles with lectin binding sites. Janus droplets orient in a vertical direction as a result of the difference in densities between the hydrocarbon and fluorocarbon solvents. Binding of lectin to mannose(s) causes agglutination and a tilted geometry. The distinct optical difference between naturally aligned and agglutinated Janus droplets produces signals that can be detected quantitatively. The Janus emulsion assay sensitively and selectively binds to E. coli at 10 4 cfu/mL and can be easily prepared with long-time stability. It provides the basis for the development of inexpensive portable devices for fast, on-site pathogen detection. |
| first_indexed | 2024-09-23T16:30:03Z |
| format | Article |
| id | mit-1721.1/113582 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T16:30:03Z |
| publishDate | 2018 |
| publisher | American Chemical Society (ACS) |
| record_format | dspace |
| spelling | mit-1721.1/1135822022-09-29T20:00:25Z Janus Emulsions for the Detection of Bacteria Kaplonek, Paulina Seeberger, Peter H. Zhang, Qifan Savagatrup, Suchol Swager, Timothy M Massachusetts Institute of Technology. Institute for Soldier Nanotechnologies Massachusetts Institute of Technology. Department of Chemistry Zhang, Qifan Savagatrup, Suchol Swager, Timothy M Janus emulsion assays that rely on carbohydrate-lectin binding for the detection of Escherichia coli bacteria are described. Surfactants containing mannose are self-assembled at the surface of Janus droplets to produce particles with lectin binding sites. Janus droplets orient in a vertical direction as a result of the difference in densities between the hydrocarbon and fluorocarbon solvents. Binding of lectin to mannose(s) causes agglutination and a tilted geometry. The distinct optical difference between naturally aligned and agglutinated Janus droplets produces signals that can be detected quantitatively. The Janus emulsion assay sensitively and selectively binds to E. coli at 10 4 cfu/mL and can be easily prepared with long-time stability. It provides the basis for the development of inexpensive portable devices for fast, on-site pathogen detection. 2018-02-12T16:33:22Z 2018-02-12T16:33:22Z 2017-03 2017-01 2018-02-08T17:29:22Z Article http://purl.org/eprint/type/JournalArticle 2374-7943 2374-7951 http://hdl.handle.net/1721.1/113582 Zhang, Qifan et al. “Janus Emulsions for the Detection of Bacteria.” ACS Central Science 3, 4 (March 2017): 309–313 © 2017 American Chemical Society https://orcid.org/0000-0001-8839-5091 http://dx.doi.org/10.1021/ACSCENTSCI.7B00021 ACS Central Science Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf American Chemical Society (ACS) ACS |
| spellingShingle | Kaplonek, Paulina Seeberger, Peter H. Zhang, Qifan Savagatrup, Suchol Swager, Timothy M Janus Emulsions for the Detection of Bacteria |
| title | Janus Emulsions for the Detection of Bacteria |
| title_full | Janus Emulsions for the Detection of Bacteria |
| title_fullStr | Janus Emulsions for the Detection of Bacteria |
| title_full_unstemmed | Janus Emulsions for the Detection of Bacteria |
| title_short | Janus Emulsions for the Detection of Bacteria |
| title_sort | janus emulsions for the detection of bacteria |
| url | http://hdl.handle.net/1721.1/113582 https://orcid.org/0000-0001-8839-5091 |
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