Chirality dependent corona phase molecular recognition of DNA-wrapped carbon nanotubes
Corona phase molecular recognition (CoPhMoRe) is a phenomenon whereby a polymer or surfactant corona phase wrapped around a nanoparticle selectively recognizes a particular molecule. The method can potentially generate non-biological, synthetic molecular recognition sites, analogous to antibodies, f...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | en_US |
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Elsevier
2018
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| Online Access: | http://hdl.handle.net/1721.1/113650 https://orcid.org/0000-0002-6349-0719 https://orcid.org/0000-0002-5832-8522 https://orcid.org/0000-0003-2592-7956 https://orcid.org/0000-0002-0739-8352 https://orcid.org/0000-0003-2944-808X |
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| author | Kruss, Sebastian Salem, Daniel Parker Landry, Markita P Bisker Raviv, Gili Hana Ahn, Jiyoung Strano, Michael S. |
| author2 | Massachusetts Institute of Technology. Department of Chemistry |
| author_facet | Massachusetts Institute of Technology. Department of Chemistry Kruss, Sebastian Salem, Daniel Parker Landry, Markita P Bisker Raviv, Gili Hana Ahn, Jiyoung Strano, Michael S. |
| author_sort | Kruss, Sebastian |
| collection | MIT |
| description | Corona phase molecular recognition (CoPhMoRe) is a phenomenon whereby a polymer or surfactant corona phase wrapped around a nanoparticle selectively recognizes a particular molecule. The method can potentially generate non-biological, synthetic molecular recognition sites, analogous to antibodies, for a broad range of biomedical applications, including new types of sensors, laboratory and clinical assays, as well as inhibitors and targeted therapeutics. In this work, we utilize near infrared fluorescent single-walled carbon nanotubes (SWNTs) wrapped with specific single stranded DNA sequences to explore the (n,m) chirality dependence of CoPhMoRe. Specific DNA oligonucleotide sequences are known to recognize and interact uniquely with certain (n,m) SWNTs enabling their enrichment in ion exchange chromatography. We explore the CoPhMoRe effect using corona phases constructed from a library of 24 such sequences, screening against a biomolecule panel that includes common neurotransmitters, amino acids, saccharides and riboflavin. Example sequences include (ATT)₄, (TAT)₄ and (ATTT)₃ which recognize (7,5), (6,5) and (8,4) SWNTs, respectively. We find that these recognition sequences indeed form CoPhMoRe phases that are distinct among SWNT chiralities, and appear to pack more densely as to exclude analyte adsorption on the chirality they recognize. These results have encouraging implications for the controlled design of CoPhMoRe phases for biomedical applications. |
| first_indexed | 2024-09-23T10:43:04Z |
| format | Article |
| id | mit-1721.1/113650 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T10:43:04Z |
| publishDate | 2018 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | mit-1721.1/1136502022-09-27T14:30:27Z Chirality dependent corona phase molecular recognition of DNA-wrapped carbon nanotubes Kruss, Sebastian Salem, Daniel Parker Landry, Markita P Bisker Raviv, Gili Hana Ahn, Jiyoung Strano, Michael S. Massachusetts Institute of Technology. Department of Chemistry Strano, Michael S. Salem, Daniel Parker Landry, Markita P Bisker Raviv, Gili Hana Ahn, Jiyoung Strano, Michael S. Corona phase molecular recognition (CoPhMoRe) is a phenomenon whereby a polymer or surfactant corona phase wrapped around a nanoparticle selectively recognizes a particular molecule. The method can potentially generate non-biological, synthetic molecular recognition sites, analogous to antibodies, for a broad range of biomedical applications, including new types of sensors, laboratory and clinical assays, as well as inhibitors and targeted therapeutics. In this work, we utilize near infrared fluorescent single-walled carbon nanotubes (SWNTs) wrapped with specific single stranded DNA sequences to explore the (n,m) chirality dependence of CoPhMoRe. Specific DNA oligonucleotide sequences are known to recognize and interact uniquely with certain (n,m) SWNTs enabling their enrichment in ion exchange chromatography. We explore the CoPhMoRe effect using corona phases constructed from a library of 24 such sequences, screening against a biomolecule panel that includes common neurotransmitters, amino acids, saccharides and riboflavin. Example sequences include (ATT)₄, (TAT)₄ and (ATTT)₃ which recognize (7,5), (6,5) and (8,4) SWNTs, respectively. We find that these recognition sequences indeed form CoPhMoRe phases that are distinct among SWNT chiralities, and appear to pack more densely as to exclude analyte adsorption on the chirality they recognize. These results have encouraging implications for the controlled design of CoPhMoRe phases for biomedical applications. National Science Foundation (U.S.) (Grant 2388357) National Science Foundation (U.S.) (Award 1306229) National Science Foundation (U.S.) (Award 1213622) 2018-02-14T14:46:12Z 2018-02-14T14:46:12Z 2015-08 2015-08 Article http://purl.org/eprint/type/JournalArticle 0008-6223 http://hdl.handle.net/1721.1/113650 Salem, Daniel P. et al. “Chirality Dependent Corona Phase Molecular Recognition of DNA-Wrapped Carbon Nanotubes.” Carbon 97 (February 2016): 147–153 © 2015 Elsevier Ltd https://orcid.org/0000-0002-6349-0719 https://orcid.org/0000-0002-5832-8522 https://orcid.org/0000-0003-2592-7956 https://orcid.org/0000-0002-0739-8352 https://orcid.org/0000-0003-2944-808X en_US http://dx.doi.org/10.1016/j.carbon.2015.08.075 Carbon Creative Commons Attribution-NonCommercial-NoDerivs License http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Elsevier Prof. Strano via Erja Kajosalo |
| spellingShingle | Kruss, Sebastian Salem, Daniel Parker Landry, Markita P Bisker Raviv, Gili Hana Ahn, Jiyoung Strano, Michael S. Chirality dependent corona phase molecular recognition of DNA-wrapped carbon nanotubes |
| title | Chirality dependent corona phase molecular recognition of DNA-wrapped carbon nanotubes |
| title_full | Chirality dependent corona phase molecular recognition of DNA-wrapped carbon nanotubes |
| title_fullStr | Chirality dependent corona phase molecular recognition of DNA-wrapped carbon nanotubes |
| title_full_unstemmed | Chirality dependent corona phase molecular recognition of DNA-wrapped carbon nanotubes |
| title_short | Chirality dependent corona phase molecular recognition of DNA-wrapped carbon nanotubes |
| title_sort | chirality dependent corona phase molecular recognition of dna wrapped carbon nanotubes |
| url | http://hdl.handle.net/1721.1/113650 https://orcid.org/0000-0002-6349-0719 https://orcid.org/0000-0002-5832-8522 https://orcid.org/0000-0003-2592-7956 https://orcid.org/0000-0002-0739-8352 https://orcid.org/0000-0003-2944-808X |
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