Comparative ribosome profiling uncovers a dominant role for translational control in Toxoplasma gondii
Background: The lytic cycle of the protozoan parasite Toxoplasma gondii, which involves a brief sojourn in the extracellular space, is characterized by defined transcriptional profiles. For an obligate intracellular parasite that is shielded from the cytosolic host immune factors by a parasitophorou...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BioMed Central
2018
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| Online Access: | http://hdl.handle.net/1721.1/114431 |
| _version_ | 1811098028952518656 |
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| author | Hassan, Musa A. Guo-Liang, Chew Meissner, Markus Nicolai Siegel, T. Vasquez, Juan J. Vasquez Ospina, Juan Jose |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Hassan, Musa A. Guo-Liang, Chew Meissner, Markus Nicolai Siegel, T. Vasquez, Juan J. Vasquez Ospina, Juan Jose |
| author_sort | Hassan, Musa A. |
| collection | MIT |
| description | Background: The lytic cycle of the protozoan parasite Toxoplasma gondii, which involves a brief sojourn in the extracellular space, is characterized by defined transcriptional profiles. For an obligate intracellular parasite that is shielded from the cytosolic host immune factors by a parasitophorous vacuole, the brief entry into the extracellular space is likely to exert enormous stress. Due to its role in cellular stress response, we hypothesize that translational control plays an important role in regulating gene expression in Toxoplasma during the lytic cycle. Unlike transcriptional profiles, insights into genome-wide translational profiles of Toxoplasma gondii are lacking.
Methods: We have performed genome-wide ribosome profiling, coupled with high throughput RNA sequencing, in intracellular and extracellular Toxoplasma gondii parasites to investigate translational control during the lytic cycle.
Results: Although differences in transcript abundance were mostly mirrored at the translational level, we observed significant differences in the abundance of ribosome footprints between the two parasite stages. Furthermore, our data suggest that mRNA translation in the parasite is potentially regulated by mRNA secondary structure and upstream open reading frames.
Conclusion: We show that most of the Toxoplasma genes that are dysregulated during the lytic cycle are translationally regulated. Keywords: ribosome profiling; RNA-sequencing; translation efficiency; toxoplasma gondii; apicomplexan |
| first_indexed | 2024-09-23T17:08:50Z |
| format | Article |
| id | mit-1721.1/114431 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T17:08:50Z |
| publishDate | 2018 |
| publisher | BioMed Central |
| record_format | dspace |
| spelling | mit-1721.1/1144312022-10-03T10:44:54Z Comparative ribosome profiling uncovers a dominant role for translational control in Toxoplasma gondii Hassan, Musa A. Guo-Liang, Chew Meissner, Markus Nicolai Siegel, T. Vasquez, Juan J. Vasquez Ospina, Juan Jose Massachusetts Institute of Technology. Department of Biological Engineering Vasquez Ospina, Juan Jose Background: The lytic cycle of the protozoan parasite Toxoplasma gondii, which involves a brief sojourn in the extracellular space, is characterized by defined transcriptional profiles. For an obligate intracellular parasite that is shielded from the cytosolic host immune factors by a parasitophorous vacuole, the brief entry into the extracellular space is likely to exert enormous stress. Due to its role in cellular stress response, we hypothesize that translational control plays an important role in regulating gene expression in Toxoplasma during the lytic cycle. Unlike transcriptional profiles, insights into genome-wide translational profiles of Toxoplasma gondii are lacking. Methods: We have performed genome-wide ribosome profiling, coupled with high throughput RNA sequencing, in intracellular and extracellular Toxoplasma gondii parasites to investigate translational control during the lytic cycle. Results: Although differences in transcript abundance were mostly mirrored at the translational level, we observed significant differences in the abundance of ribosome footprints between the two parasite stages. Furthermore, our data suggest that mRNA translation in the parasite is potentially regulated by mRNA secondary structure and upstream open reading frames. Conclusion: We show that most of the Toxoplasma genes that are dysregulated during the lytic cycle are translationally regulated. Keywords: ribosome profiling; RNA-sequencing; translation efficiency; toxoplasma gondii; apicomplexan 2018-03-28T18:44:09Z 2018-03-28T18:44:09Z 2017-12 2017-07 2017-12-17T04:53:11Z Article http://purl.org/eprint/type/JournalArticle 1471-2164 http://hdl.handle.net/1721.1/114431 Hassan, Musa A. et al. "Comparative ribosome profiling uncovers a dominant role for translational control in Toxoplasma gondii." BMC Genomics 18 (December 2017): 961 © 2017 The Author(s) en http://dx.doi.org/10.1186/s12864-017-4362-6 BMC Genomics Creative Commons Attribution http://creativecommons.org/licenses/by/4.0/ The Author(s). application/pdf BioMed Central BioMed Central |
| spellingShingle | Hassan, Musa A. Guo-Liang, Chew Meissner, Markus Nicolai Siegel, T. Vasquez, Juan J. Vasquez Ospina, Juan Jose Comparative ribosome profiling uncovers a dominant role for translational control in Toxoplasma gondii |
| title | Comparative ribosome profiling uncovers a dominant role for translational control in Toxoplasma gondii |
| title_full | Comparative ribosome profiling uncovers a dominant role for translational control in Toxoplasma gondii |
| title_fullStr | Comparative ribosome profiling uncovers a dominant role for translational control in Toxoplasma gondii |
| title_full_unstemmed | Comparative ribosome profiling uncovers a dominant role for translational control in Toxoplasma gondii |
| title_short | Comparative ribosome profiling uncovers a dominant role for translational control in Toxoplasma gondii |
| title_sort | comparative ribosome profiling uncovers a dominant role for translational control in toxoplasma gondii |
| url | http://hdl.handle.net/1721.1/114431 |
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