Microfluidic Fabrication of Colloidal Nanomaterials-Encapsulated Microcapsules for Biomolecular Sensing
Implantable sensors that detect biomarkers in vivo are critical for early disease diagnostics. Although many colloidal nanomaterials have been developed into optical sensors to detect biomolecules in vitro, their application in vivo as implantable sensors is hindered by potential migration or cleara...
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| Format: | Article |
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American Chemical Society (ACS)
2018
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| Online Access: | http://hdl.handle.net/1721.1/114824 https://orcid.org/0000-0002-5108-8212 https://orcid.org/0000-0002-0739-8352 https://orcid.org/0000-0001-8223-035X https://orcid.org/0000-0002-5921-3436 https://orcid.org/0000-0001-9522-8208 https://orcid.org/0000-0001-7779-0424 https://orcid.org/0000-0002-5166-1410 https://orcid.org/0000-0003-2592-7956 https://orcid.org/0000-0002-7635-5102 https://orcid.org/0000-0003-2944-808X https://orcid.org/0000-0001-5629-4798 |
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| author | Zhang, Weixia Abbaspourrad, Alireza Tao, Jun Hang, Tian Weitz, David A. Xie, Xi Ahn, Jiyoung Bader, Andrew Bose, Suman Vegas, Arturo Lin, Jiaqi Iverson, Nicole M. Bisker Raviv, Gili Hana Li, Linxian Strano, Michael S. Anderson, Daniel Griffith Lee, Hyomin, Ph. D. Massachusetts Institute of Technology |
| author2 | Massachusetts Institute of Technology. Institute for Medical Engineering & Science |
| author_facet | Massachusetts Institute of Technology. Institute for Medical Engineering & Science Zhang, Weixia Abbaspourrad, Alireza Tao, Jun Hang, Tian Weitz, David A. Xie, Xi Ahn, Jiyoung Bader, Andrew Bose, Suman Vegas, Arturo Lin, Jiaqi Iverson, Nicole M. Bisker Raviv, Gili Hana Li, Linxian Strano, Michael S. Anderson, Daniel Griffith Lee, Hyomin, Ph. D. Massachusetts Institute of Technology |
| author_sort | Zhang, Weixia |
| collection | MIT |
| description | Implantable sensors that detect biomarkers in vivo are critical for early disease diagnostics. Although many colloidal nanomaterials have been developed into optical sensors to detect biomolecules in vitro, their application in vivo as implantable sensors is hindered by potential migration or clearance from the implantation site. One potential solution is incorporating colloidal nanosensors in hydrogel scaffold prior to implantation. However, direct contact between the nanosensors and hydrogel matrix has the potential to disrupt sensor performance. Here, we develop a hollow-microcapsule-based sensing platform that protects colloidal nanosensors from direct contact with hydrogel matrix. Using microfluidics, colloidal nanosensors were encapsulated in polyethylene glycol microcapsules with liquid cores. The microcapsules selectively trap the nanosensors within the core while allowing free diffusion of smaller molecules such as glucose and heparin. Glucose-responsive quantum dots or gold nanorods or heparin-responsive gold nanorods were each encapsulated. Microcapsules loaded with these sensors showed responsive optical signals in the presence of target biomolecules (glucose or heparin). Furthermore, these microcapsules can be immobilized into biocompatible hydrogel as implantable devices for biomolecular sensing. This technique offers new opportunities to extend the utility of colloidal nanosensors from solution-based detection to implantable device-based detection. Keywords: biomolecular sensing; Microcapsules; microfluidic fabrication; nanosensors |
| first_indexed | 2024-09-23T11:44:38Z |
| format | Article |
| id | mit-1721.1/114824 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T11:44:38Z |
| publishDate | 2018 |
| publisher | American Chemical Society (ACS) |
| record_format | dspace |
| spelling | mit-1721.1/1148242022-09-27T21:36:31Z Microfluidic Fabrication of Colloidal Nanomaterials-Encapsulated Microcapsules for Biomolecular Sensing Zhang, Weixia Abbaspourrad, Alireza Tao, Jun Hang, Tian Weitz, David A. Xie, Xi Ahn, Jiyoung Bader, Andrew Bose, Suman Vegas, Arturo Lin, Jiaqi Iverson, Nicole M. Bisker Raviv, Gili Hana Li, Linxian Strano, Michael S. Anderson, Daniel Griffith Lee, Hyomin, Ph. D. Massachusetts Institute of Technology Massachusetts Institute of Technology. Institute for Medical Engineering & Science Harvard University--MIT Division of Health Sciences and Technology Massachusetts Institute of Technology. Department of Chemical Engineering Massachusetts Institute of Technology. Department of Physics Koch Institute for Integrative Cancer Research at MIT Xie, Xi Ahn, Jiyoung Bader, Andrew Bose, Suman Vegas, Arturo Lin, Jiaqi Iverson, Nicole M. Bisker Raviv, Gili Hana Li, Linxian Strano, Michael S. Anderson, Daniel Griffith Implantable sensors that detect biomarkers in vivo are critical for early disease diagnostics. Although many colloidal nanomaterials have been developed into optical sensors to detect biomolecules in vitro, their application in vivo as implantable sensors is hindered by potential migration or clearance from the implantation site. One potential solution is incorporating colloidal nanosensors in hydrogel scaffold prior to implantation. However, direct contact between the nanosensors and hydrogel matrix has the potential to disrupt sensor performance. Here, we develop a hollow-microcapsule-based sensing platform that protects colloidal nanosensors from direct contact with hydrogel matrix. Using microfluidics, colloidal nanosensors were encapsulated in polyethylene glycol microcapsules with liquid cores. The microcapsules selectively trap the nanosensors within the core while allowing free diffusion of smaller molecules such as glucose and heparin. Glucose-responsive quantum dots or gold nanorods or heparin-responsive gold nanorods were each encapsulated. Microcapsules loaded with these sensors showed responsive optical signals in the presence of target biomolecules (glucose or heparin). Furthermore, these microcapsules can be immobilized into biocompatible hydrogel as implantable devices for biomolecular sensing. This technique offers new opportunities to extend the utility of colloidal nanosensors from solution-based detection to implantable device-based detection. Keywords: biomolecular sensing; Microcapsules; microfluidic fabrication; nanosensors Juvenile Diabetes Research Foundation International (Award 17-2013-507) 2018-04-20T19:04:59Z 2018-04-20T19:04:59Z 2017-02 2017-01 2018-04-19T14:27:28Z Article http://purl.org/eprint/type/JournalArticle 1530-6984 1530-6992 http://hdl.handle.net/1721.1/114824 Xie, Xi et al. “Microfluidic Fabrication of Colloidal Nanomaterials-Encapsulated Microcapsules for Biomolecular Sensing.” Nano Letters 17, 3 (February 2017): 2015–2020 © 2017 American Chemical Society https://orcid.org/0000-0002-5108-8212 https://orcid.org/0000-0002-0739-8352 https://orcid.org/0000-0001-8223-035X https://orcid.org/0000-0002-5921-3436 https://orcid.org/0000-0001-9522-8208 https://orcid.org/0000-0001-7779-0424 https://orcid.org/0000-0002-5166-1410 https://orcid.org/0000-0003-2592-7956 https://orcid.org/0000-0002-7635-5102 https://orcid.org/0000-0003-2944-808X https://orcid.org/0000-0001-5629-4798 http://dx.doi.org/10.1021/ACS.NANOLETT.7B00026 Nano Letters Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf American Chemical Society (ACS) Other repository |
| spellingShingle | Zhang, Weixia Abbaspourrad, Alireza Tao, Jun Hang, Tian Weitz, David A. Xie, Xi Ahn, Jiyoung Bader, Andrew Bose, Suman Vegas, Arturo Lin, Jiaqi Iverson, Nicole M. Bisker Raviv, Gili Hana Li, Linxian Strano, Michael S. Anderson, Daniel Griffith Lee, Hyomin, Ph. D. Massachusetts Institute of Technology Microfluidic Fabrication of Colloidal Nanomaterials-Encapsulated Microcapsules for Biomolecular Sensing |
| title | Microfluidic Fabrication of Colloidal Nanomaterials-Encapsulated Microcapsules for Biomolecular Sensing |
| title_full | Microfluidic Fabrication of Colloidal Nanomaterials-Encapsulated Microcapsules for Biomolecular Sensing |
| title_fullStr | Microfluidic Fabrication of Colloidal Nanomaterials-Encapsulated Microcapsules for Biomolecular Sensing |
| title_full_unstemmed | Microfluidic Fabrication of Colloidal Nanomaterials-Encapsulated Microcapsules for Biomolecular Sensing |
| title_short | Microfluidic Fabrication of Colloidal Nanomaterials-Encapsulated Microcapsules for Biomolecular Sensing |
| title_sort | microfluidic fabrication of colloidal nanomaterials encapsulated microcapsules for biomolecular sensing |
| url | http://hdl.handle.net/1721.1/114824 https://orcid.org/0000-0002-5108-8212 https://orcid.org/0000-0002-0739-8352 https://orcid.org/0000-0001-8223-035X https://orcid.org/0000-0002-5921-3436 https://orcid.org/0000-0001-9522-8208 https://orcid.org/0000-0001-7779-0424 https://orcid.org/0000-0002-5166-1410 https://orcid.org/0000-0003-2592-7956 https://orcid.org/0000-0002-7635-5102 https://orcid.org/0000-0003-2944-808X https://orcid.org/0000-0001-5629-4798 |
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