Isolation of exosomes from whole blood by integrating acoustics and microfluidics
Exosomes are nanoscale extracellular vesicles that play an important role in many biological processes, including intercellular communications, antigen presentation, and the transport of proteins, RNA, and other molecules. Recently there has been significant interest in exosome-related fundamental r...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
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National Academy of Sciences (U.S.)
2018
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| Online Access: | http://hdl.handle.net/1721.1/114913 https://orcid.org/0000-0001-5372-385X |
| _version_ | 1826196324792401920 |
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| author | Wu, Mengxi Ouyang, Yingshi Wang, Zeyu Zhang, Rui Huang, Po-Hsun Chen, Chuyi Li, Hui Li, Peng Quinn, David Suresh, Subra Sadovsky, Yoel Huang, Tony Jun Dao, Ming |
| author2 | Massachusetts Institute of Technology. Department of Materials Science and Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Materials Science and Engineering Wu, Mengxi Ouyang, Yingshi Wang, Zeyu Zhang, Rui Huang, Po-Hsun Chen, Chuyi Li, Hui Li, Peng Quinn, David Suresh, Subra Sadovsky, Yoel Huang, Tony Jun Dao, Ming |
| author_sort | Wu, Mengxi |
| collection | MIT |
| description | Exosomes are nanoscale extracellular vesicles that play an important role in many biological processes, including intercellular communications, antigen presentation, and the transport of proteins, RNA, and other molecules. Recently there has been significant interest in exosome-related fundamental research, seeking new exosome-based biomarkers for health monitoring and disease diagnoses. Here, we report a separation method based on acoustofluidics (i.e., the integration of acoustics and microfluidics) to isolate exosomes directly from whole blood in a label-free and contact-free manner. This acoustofluidic platform consists of two modules: a microscale cell-removal module that first removes larger blood components, followed by extracellular vesicle subgroup separation in the exosome-isolation module. In the cell-removal module, we demonstrate the isolation of 110-nm particles from a mixture of micro- and nanosized particles with a yield greater than 99%. In the exosome-isolation module, we isolate exosomes from an extracellular vesicle mixture with a purity of 98.4%. Integrating the two acoustofluidic modules onto a single chip, we isolated exosomes from whole blood with a blood cell removal rate of over 99.999%. With its ability to perform rapid, biocompatible, label-free, contact-free, and continuous-flow exosome isolation, the integrated acoustofluidic device offers a unique approach to investigate the role of exosomes in the onset and progression of human diseases with potential applications in health monitoring, medical diagnosis, targeted drug delivery, and personalized medicine. Keywords: extracellular vesicles; exosomes; blood-borne vesicles; surface acoustic waves; acoustic tweezers |
| first_indexed | 2024-09-23T10:25:07Z |
| format | Article |
| id | mit-1721.1/114913 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T10:25:07Z |
| publishDate | 2018 |
| publisher | National Academy of Sciences (U.S.) |
| record_format | dspace |
| spelling | mit-1721.1/1149132022-09-26T17:45:58Z Isolation of exosomes from whole blood by integrating acoustics and microfluidics Wu, Mengxi Ouyang, Yingshi Wang, Zeyu Zhang, Rui Huang, Po-Hsun Chen, Chuyi Li, Hui Li, Peng Quinn, David Suresh, Subra Sadovsky, Yoel Huang, Tony Jun Dao, Ming Massachusetts Institute of Technology. Department of Materials Science and Engineering Dao, Ming Exosomes are nanoscale extracellular vesicles that play an important role in many biological processes, including intercellular communications, antigen presentation, and the transport of proteins, RNA, and other molecules. Recently there has been significant interest in exosome-related fundamental research, seeking new exosome-based biomarkers for health monitoring and disease diagnoses. Here, we report a separation method based on acoustofluidics (i.e., the integration of acoustics and microfluidics) to isolate exosomes directly from whole blood in a label-free and contact-free manner. This acoustofluidic platform consists of two modules: a microscale cell-removal module that first removes larger blood components, followed by extracellular vesicle subgroup separation in the exosome-isolation module. In the cell-removal module, we demonstrate the isolation of 110-nm particles from a mixture of micro- and nanosized particles with a yield greater than 99%. In the exosome-isolation module, we isolate exosomes from an extracellular vesicle mixture with a purity of 98.4%. Integrating the two acoustofluidic modules onto a single chip, we isolated exosomes from whole blood with a blood cell removal rate of over 99.999%. With its ability to perform rapid, biocompatible, label-free, contact-free, and continuous-flow exosome isolation, the integrated acoustofluidic device offers a unique approach to investigate the role of exosomes in the onset and progression of human diseases with potential applications in health monitoring, medical diagnosis, targeted drug delivery, and personalized medicine. Keywords: extracellular vesicles; exosomes; blood-borne vesicles; surface acoustic waves; acoustic tweezers National Science Foundation (U.S.) (Grant R01 HD086325) National Science Foundation (U.S.) (Grant IIP-1534645) 2018-04-24T13:28:22Z 2018-04-24T13:28:22Z 2017-09 2017-06 2018-04-20T13:28:35Z Article http://purl.org/eprint/type/ConferencePaper 0027-8424 1091-6490 http://hdl.handle.net/1721.1/114913 Wu, Mengxi et al. “Isolation of Exosomes from Whole Blood by Integrating Acoustics and Microfluidics.” Proceedings of the National Academy of Sciences 114, 40 (September 2017): 10584–10589 © 2017 National Academy of Sciences https://orcid.org/0000-0001-5372-385X http://dx.doi.org/10.1073/PNAS.1709210114 Proceedings of the National Academy of Sciences Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf National Academy of Sciences (U.S.) National Academy of Sciences |
| spellingShingle | Wu, Mengxi Ouyang, Yingshi Wang, Zeyu Zhang, Rui Huang, Po-Hsun Chen, Chuyi Li, Hui Li, Peng Quinn, David Suresh, Subra Sadovsky, Yoel Huang, Tony Jun Dao, Ming Isolation of exosomes from whole blood by integrating acoustics and microfluidics |
| title | Isolation of exosomes from whole blood by integrating acoustics and microfluidics |
| title_full | Isolation of exosomes from whole blood by integrating acoustics and microfluidics |
| title_fullStr | Isolation of exosomes from whole blood by integrating acoustics and microfluidics |
| title_full_unstemmed | Isolation of exosomes from whole blood by integrating acoustics and microfluidics |
| title_short | Isolation of exosomes from whole blood by integrating acoustics and microfluidics |
| title_sort | isolation of exosomes from whole blood by integrating acoustics and microfluidics |
| url | http://hdl.handle.net/1721.1/114913 https://orcid.org/0000-0001-5372-385X |
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