Organization of Valence-Encoding and Projection-Defined Neurons in the Basolateral Amygdala
The basolateral amygdala (BLA) mediates associative learning for both fear and reward. Accumulating evidence supports the notion that different BLA projections distinctly alter motivated behavior, including projections to the nucleus accumbens (NAc), medial aspect of the central amygdala (CeM), and...
| Main Authors: | , , , , , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Published: |
Elsevier
2018
|
| Online Access: | http://hdl.handle.net/1721.1/114932 https://orcid.org/0000-0003-2371-5706 https://orcid.org/0000-0003-0766-403X https://orcid.org/0000-0002-1410-8675 |
| _version_ | 1811094970085408768 |
|---|---|
| author | Beyeler, Anna Chang, Chia-Jung Silvestre, Margaux Leveque, Clementine Namburi, Praneeth Wildes, Craig P Tye, Kay M |
| author2 | Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences |
| author_facet | Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Beyeler, Anna Chang, Chia-Jung Silvestre, Margaux Leveque, Clementine Namburi, Praneeth Wildes, Craig P Tye, Kay M |
| author_sort | Beyeler, Anna |
| collection | MIT |
| description | The basolateral amygdala (BLA) mediates associative learning for both fear and reward. Accumulating evidence supports the notion that different BLA projections distinctly alter motivated behavior, including projections to the nucleus accumbens (NAc), medial aspect of the central amygdala (CeM), and ventral hippocampus (vHPC). Although there is consensus regarding the existence of distinct subsets of BLA neurons encoding positive or negative valence, controversy remains regarding the anatomical arrangement of these populations. First, we map the location of more than 1,000 neurons distributed across the BLA and recorded during a Pavlovian discrimination task. Next, we determine the location of projection-defined neurons labeled with retrograde tracers and use CLARITY to reveal the axonal path in 3-dimensional space. Finally, we examine the local influence of each projection-defined populations within the BLA. Understanding the functional and topographical organization of circuits underlying valence assignment could reveal fundamental principles about emotional processing. Basolateral amygdala (BLA) neurons distinctly encode cues predicting rewards or punishments, but how does form give rise to function? Beyeler et al. overlay anatomical projection target, location of neurons in a 3D map, and encoding properties during cue discrimination. The influence on local networks differs across projection-defined BLA populations. Keywords: reward; aversion; topography; tracing; connectivity; network; channelrhodopsin; phototagging; photoexcitation; photoinhition |
| first_indexed | 2024-09-23T16:08:06Z |
| format | Article |
| id | mit-1721.1/114932 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T16:08:06Z |
| publishDate | 2018 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | mit-1721.1/1149322022-09-29T18:29:13Z Organization of Valence-Encoding and Projection-Defined Neurons in the Basolateral Amygdala Beyeler, Anna Chang, Chia-Jung Silvestre, Margaux Leveque, Clementine Namburi, Praneeth Wildes, Craig P Tye, Kay M Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Picower Institute for Learning and Memory Beyeler, Anna Chang, Chia-Jung Silvestre, Margaux Leveque, Clementine Namburi, Praneeth Wildes, Craig P Tye, Kay M The basolateral amygdala (BLA) mediates associative learning for both fear and reward. Accumulating evidence supports the notion that different BLA projections distinctly alter motivated behavior, including projections to the nucleus accumbens (NAc), medial aspect of the central amygdala (CeM), and ventral hippocampus (vHPC). Although there is consensus regarding the existence of distinct subsets of BLA neurons encoding positive or negative valence, controversy remains regarding the anatomical arrangement of these populations. First, we map the location of more than 1,000 neurons distributed across the BLA and recorded during a Pavlovian discrimination task. Next, we determine the location of projection-defined neurons labeled with retrograde tracers and use CLARITY to reveal the axonal path in 3-dimensional space. Finally, we examine the local influence of each projection-defined populations within the BLA. Understanding the functional and topographical organization of circuits underlying valence assignment could reveal fundamental principles about emotional processing. Basolateral amygdala (BLA) neurons distinctly encode cues predicting rewards or punishments, but how does form give rise to function? Beyeler et al. overlay anatomical projection target, location of neurons in a 3D map, and encoding properties during cue discrimination. The influence on local networks differs across projection-defined BLA populations. Keywords: reward; aversion; topography; tracing; connectivity; network; channelrhodopsin; phototagging; photoexcitation; photoinhition National Institute of Mental Health (U.S.) (Grant R01-MH102441) National Institute of Diabetes and Digestive and Kidney Diseases (U.S.) (Award DP2-DK-102256) 2018-04-24T16:56:17Z 2018-04-24T16:56:17Z 2018-01 2017-10 2018-04-20T18:41:37Z Article http://purl.org/eprint/type/JournalArticle 2211-1247 http://hdl.handle.net/1721.1/114932 Beyeler, Anna et al. “Organization of Valence-Encoding and Projection-Defined Neurons in the Basolateral Amygdala.” Cell Reports 22, 4 (January 2018): 905–918 © 2018 The Authors https://orcid.org/0000-0003-2371-5706 https://orcid.org/0000-0003-0766-403X https://orcid.org/0000-0002-1410-8675 http://dx.doi.org/10.1016/j.celrep.2017.12.097 Cell Reports Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) https://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Elsevier Cell Reports |
| spellingShingle | Beyeler, Anna Chang, Chia-Jung Silvestre, Margaux Leveque, Clementine Namburi, Praneeth Wildes, Craig P Tye, Kay M Organization of Valence-Encoding and Projection-Defined Neurons in the Basolateral Amygdala |
| title | Organization of Valence-Encoding and Projection-Defined Neurons in the Basolateral Amygdala |
| title_full | Organization of Valence-Encoding and Projection-Defined Neurons in the Basolateral Amygdala |
| title_fullStr | Organization of Valence-Encoding and Projection-Defined Neurons in the Basolateral Amygdala |
| title_full_unstemmed | Organization of Valence-Encoding and Projection-Defined Neurons in the Basolateral Amygdala |
| title_short | Organization of Valence-Encoding and Projection-Defined Neurons in the Basolateral Amygdala |
| title_sort | organization of valence encoding and projection defined neurons in the basolateral amygdala |
| url | http://hdl.handle.net/1721.1/114932 https://orcid.org/0000-0003-2371-5706 https://orcid.org/0000-0003-0766-403X https://orcid.org/0000-0002-1410-8675 |
| work_keys_str_mv | AT beyeleranna organizationofvalenceencodingandprojectiondefinedneuronsinthebasolateralamygdala AT changchiajung organizationofvalenceencodingandprojectiondefinedneuronsinthebasolateralamygdala AT silvestremargaux organizationofvalenceencodingandprojectiondefinedneuronsinthebasolateralamygdala AT levequeclementine organizationofvalenceencodingandprojectiondefinedneuronsinthebasolateralamygdala AT namburipraneeth organizationofvalenceencodingandprojectiondefinedneuronsinthebasolateralamygdala AT wildescraigp organizationofvalenceencodingandprojectiondefinedneuronsinthebasolateralamygdala AT tyekaym organizationofvalenceencodingandprojectiondefinedneuronsinthebasolateralamygdala |