Nanoparticle-antagomiR based targeting of miR-31 to induce osterix and osteocalcin expression in mesenchymal stem cells
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Mesenchymal stem cells are multipotent adult stem cells capable...
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Public Library of Science
2018
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| Online Access: | http://hdl.handle.net/1721.1/114944 https://orcid.org/0000-0001-8422-6792 |
| _version_ | 1826200854142648320 |
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| author | McCully, Mark V. Baptista, Pedro Mullin, Margaret Dalby, Matthew J. Berry, Catherine C. Osorio De Castro Conde, Joao |
| author2 | Institute for Medical Engineering and Science |
| author_facet | Institute for Medical Engineering and Science McCully, Mark V. Baptista, Pedro Mullin, Margaret Dalby, Matthew J. Berry, Catherine C. Osorio De Castro Conde, Joao |
| author_sort | McCully, Mark |
| collection | MIT |
| description | This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Mesenchymal stem cells are multipotent adult stem cells capable of generating bone, cartilage and fat, and are thus currently being exploited for regenerative medicine. When considering osteogenesis, developments have been made with regards to chemical induction (e.g. differentiation media) and physical induction (e.g. material stiffness, nanotopography), targeting established early transcription factors or regulators such as runx2 or bone morphogenic proteins and promoting increased numbers of cells committing to osteo-specific differentiation. Recent research highlighted the involvement of microRNAs in lineage commitment and terminal differentiation. Herein, gold nanoparticles that confer stability to short single stranded RNAs were used to deliver MiR-31 antagomiRs to both pre-osteoblastic cells and primary human MSCs in vitro. Results showed that blocking miR-31 led to an increase in osterix protein in both cell types at day 7, with an increase in osteocalcin at day 21, suggesting MSC osteogenesis. In addition, it was noted that antagomiR sequence direction was important, with the 5 prime reading direction proving more effective than the 3 prime. This study highlights the potential that miRNA antagomiR-Tagged nanoparticles offer as novel therapeutics in regenerative medicine. |
| first_indexed | 2024-09-23T11:42:46Z |
| format | Article |
| id | mit-1721.1/114944 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T11:42:46Z |
| publishDate | 2018 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | mit-1721.1/1149442022-10-01T05:28:02Z Nanoparticle-antagomiR based targeting of miR-31 to induce osterix and osteocalcin expression in mesenchymal stem cells McCully, Mark V. Baptista, Pedro Mullin, Margaret Dalby, Matthew J. Berry, Catherine C. Osorio De Castro Conde, Joao Institute for Medical Engineering and Science Harvard University--MIT Division of Health Sciences and Technology Osorio De Castro Conde, Joao This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Mesenchymal stem cells are multipotent adult stem cells capable of generating bone, cartilage and fat, and are thus currently being exploited for regenerative medicine. When considering osteogenesis, developments have been made with regards to chemical induction (e.g. differentiation media) and physical induction (e.g. material stiffness, nanotopography), targeting established early transcription factors or regulators such as runx2 or bone morphogenic proteins and promoting increased numbers of cells committing to osteo-specific differentiation. Recent research highlighted the involvement of microRNAs in lineage commitment and terminal differentiation. Herein, gold nanoparticles that confer stability to short single stranded RNAs were used to deliver MiR-31 antagomiRs to both pre-osteoblastic cells and primary human MSCs in vitro. Results showed that blocking miR-31 led to an increase in osterix protein in both cell types at day 7, with an increase in osteocalcin at day 21, suggesting MSC osteogenesis. In addition, it was noted that antagomiR sequence direction was important, with the 5 prime reading direction proving more effective than the 3 prime. This study highlights the potential that miRNA antagomiR-Tagged nanoparticles offer as novel therapeutics in regenerative medicine. 2018-04-24T18:41:15Z 2018-04-24T18:41:15Z 2018-02 2017-11 2018-04-20T18:14:13Z Article http://purl.org/eprint/type/JournalArticle 1932-6203 http://hdl.handle.net/1721.1/114944 McCully, Mark et al. “Nanoparticle-antagomiR Based Targeting of miR-31 to Induce Osterix and Osteocalcin Expression in Mesenchymal Stem Cells.” Edited by Hélder A. Santos. PLOS ONE 13, 2 (February 2018): e0192562 © 2018 McCully et al https://orcid.org/0000-0001-8422-6792 http://dx.doi.org/10.1371/journal.pone.0192562 PLOS ONE Creative Commons Attribution 4.0 International License https://creativecommons.org/licenses/by/4.0/ application/pdf Public Library of Science PLoS |
| spellingShingle | McCully, Mark V. Baptista, Pedro Mullin, Margaret Dalby, Matthew J. Berry, Catherine C. Osorio De Castro Conde, Joao Nanoparticle-antagomiR based targeting of miR-31 to induce osterix and osteocalcin expression in mesenchymal stem cells |
| title | Nanoparticle-antagomiR based targeting of miR-31 to induce osterix and osteocalcin expression in mesenchymal stem cells |
| title_full | Nanoparticle-antagomiR based targeting of miR-31 to induce osterix and osteocalcin expression in mesenchymal stem cells |
| title_fullStr | Nanoparticle-antagomiR based targeting of miR-31 to induce osterix and osteocalcin expression in mesenchymal stem cells |
| title_full_unstemmed | Nanoparticle-antagomiR based targeting of miR-31 to induce osterix and osteocalcin expression in mesenchymal stem cells |
| title_short | Nanoparticle-antagomiR based targeting of miR-31 to induce osterix and osteocalcin expression in mesenchymal stem cells |
| title_sort | nanoparticle antagomir based targeting of mir 31 to induce osterix and osteocalcin expression in mesenchymal stem cells |
| url | http://hdl.handle.net/1721.1/114944 https://orcid.org/0000-0001-8422-6792 |
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