Microfluidic processes to create structured microparticle arrangements and their applications
Thesis: Ph. D., Massachusetts Institute of Technology, Department of Chemical Engineering, 2017.
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| Format: | Thesis |
| Language: | eng |
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Massachusetts Institute of Technology
2018
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| Online Access: | http://hdl.handle.net/1721.1/115018 |
| _version_ | 1826192431420276736 |
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| author | Kim, Jae Jung, Ph. D. Massachusetts Institute of Technology |
| author2 | Patrick S. Doyle. |
| author_facet | Patrick S. Doyle. Kim, Jae Jung, Ph. D. Massachusetts Institute of Technology |
| author_sort | Kim, Jae Jung, Ph. D. Massachusetts Institute of Technology |
| collection | MIT |
| description | Thesis: Ph. D., Massachusetts Institute of Technology, Department of Chemical Engineering, 2017. |
| first_indexed | 2024-09-23T09:12:37Z |
| format | Thesis |
| id | mit-1721.1/115018 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T09:12:37Z |
| publishDate | 2018 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/1150182019-04-12T14:51:33Z Microfluidic processes to create structured microparticle arrangements and their applications Kim, Jae Jung, Ph. D. Massachusetts Institute of Technology Patrick S. Doyle. Massachusetts Institute of Technology. Department of Chemical Engineering. Massachusetts Institute of Technology. Department of Chemical Engineering. Chemical Engineering. Thesis: Ph. D., Massachusetts Institute of Technology, Department of Chemical Engineering, 2017. Cataloged from PDF version of thesis. Includes bibliographical references (pages 136-145). Multifunctional polymeric microparticles have shown the great potentials in a variety of fields. While the advance in particle synthesis allows for fine tuning of their physical properties and chemical functionality, particle manipulation is still appealing, but challenging issue in colloidal science. In order to expand the utility of microparticles, many particle manipulation techniques have been developed to arrange large-scale of particles at precise locations. However, current approaches cannot simultaneously fulfill desired capabilities of arrangement: scalability, precision, specificity, and versatility. This thesis explores the ability to synthesize particles with a controllability of characteristics, and development of a new microfluidic platform, porous microwell arrays, to create structured large-scale microparticle arrays using a scaling theory, which is a function of particles' characteristics. Lastly, we demonstrate the potential of generated particle arrays in various bioengineering application and material sciences. First, we synthesize anisotropic, cell-adhesive microparticles using stop flow lithography (SFL) and carbodimide coupling. Synthesized microparticles are functionalized with collagen or poly-L-lysine using streptavidin-biotin interaction, resulting in cell-adhesiveness. After functionalization, target cells are spread on the particles and spatially patterned only on the functionalized region. Thus, cells are not exposed to potentially harmful components of particle synthesis processes, photoinitiators and ultraviolet light, ensuring no physiological changes. Second, we synthesize multi-striped, upconverting nanocrystal (UCN)-laden microparticles using SFL. Distinct upconversion emission colors are combined with the ability to spatial pattern them, providing superior encoding capacities. We can fine-tune upconversion emission by controlling the dopant composition in nanocrystal, and synthesize microparticles in a highly reproducible manner by SFL, allowing for the development of predictable decoding system. Two types of particles are synthesized with this appealing encoding strategy for two distinct applications: thermally stable particles for anti-counterfeiting application; and porous hydrogels for multiplexed microRNA detection. Third, we develop a microfluidic platform, porous microwell arrays, to manipulate microparticles while fulfilling all four desired capabilities (i.e. scalability, precision, specificity, and versatility). Microwells are fabricated on top of porous membrane by a vacuum-assisted molding method. Particles are guided and assembled into wells by hydrodynamic force associated with fluid flow through pores in microwell. Iteration of assembly and washing steps ensures high-throughput, large-scale particle arrangement with high yields on filling and capturing. Scaling theory allows for the rational design of platform to specifically position microparticles depending on their physical characteristics (i.e. size, shape, and modulus), enabling to generate complex patterns. We utilize this platform in three practical applications: high-throughput, large-scale single-cell arrays; microenvironment fabrication for neutrophil chemotaxis; and UCN-laden covert 2D tags for anti-counterfeiting. Lastly, we modified the porous microwell platform to a closed system, microfluidic channels, to park and isolate particles in monodisperse droplets surrounded by fluorinated oil. Rational modification retains the platform's desired capabilities, resulting in a single particle in a droplet with high yields on both parking and isolation. Particle-in-droplet arrays enables the observation of reaction in confined volume over the time. Such arrays can be utilized to accumulate the desired product from enzymatic reaction, amplifying the signal and improving the sensitivity of bioassays. We demonstrate the highly sensitive, multiplexed miRNA detections with these particle-in-droplet arrays. by Jae Jung Kim. Ph. D. 2018-04-27T18:10:15Z 2018-04-27T18:10:15Z 2017 2017 Thesis http://hdl.handle.net/1721.1/115018 1030147632 eng MIT theses are protected by copyright. They may be viewed, downloaded, or printed from this source but further reproduction or distribution in any format is prohibited without written permission. http://dspace.mit.edu/handle/1721.1/7582 145 pages application/pdf Massachusetts Institute of Technology |
| spellingShingle | Chemical Engineering. Kim, Jae Jung, Ph. D. Massachusetts Institute of Technology Microfluidic processes to create structured microparticle arrangements and their applications |
| title | Microfluidic processes to create structured microparticle arrangements and their applications |
| title_full | Microfluidic processes to create structured microparticle arrangements and their applications |
| title_fullStr | Microfluidic processes to create structured microparticle arrangements and their applications |
| title_full_unstemmed | Microfluidic processes to create structured microparticle arrangements and their applications |
| title_short | Microfluidic processes to create structured microparticle arrangements and their applications |
| title_sort | microfluidic processes to create structured microparticle arrangements and their applications |
| topic | Chemical Engineering. |
| url | http://hdl.handle.net/1721.1/115018 |
| work_keys_str_mv | AT kimjaejungphdmassachusettsinstituteoftechnology microfluidicprocessestocreatestructuredmicroparticlearrangementsandtheirapplications |