Fundamental limits of super-resolution microscopy by dielectric microspheres and microfibers
In recent years, optical super-resolution by microspheres and microfibers emerged as a new paradigm in nanoscale label-free and fluorescence imaging. However, the mechanisms of such imaging are still not completely understood and the resolution values are debated. In this work, the fundamental limit...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
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SPIE
2018
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| Online Access: | http://hdl.handle.net/1721.1/115076 |
| _version_ | 1826206625062453248 |
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| author | Astratov, V. N. Maslov, A. V. Allen, K. W. Farahi, N. Li, Y. Brettin, A. Limberopoulos, N. I. Walker, D. E. Urbas, A. M. Liberman, Vladimir Rothschild, Mordechai |
| author2 | Lincoln Laboratory |
| author_facet | Lincoln Laboratory Astratov, V. N. Maslov, A. V. Allen, K. W. Farahi, N. Li, Y. Brettin, A. Limberopoulos, N. I. Walker, D. E. Urbas, A. M. Liberman, Vladimir Rothschild, Mordechai |
| author_sort | Astratov, V. N. |
| collection | MIT |
| description | In recent years, optical super-resolution by microspheres and microfibers emerged as a new paradigm in nanoscale label-free and fluorescence imaging. However, the mechanisms of such imaging are still not completely understood and the resolution values are debated. In this work, the fundamental limits of super-resolution imaging by high-index barium-titanate microspheres and silica microfibers are studied using nanoplasmonic arrays made from Au and Al. A rigorous resolution analysis is developed based on the object's convolution with the point-spread function that has width well below the conventional (∼λ/2) diffraction limit, where λ is the illumination wavelength. A resolution of ∼λ/6-λ/7 is demonstrated for imaging nanoplasmonic arrays by microspheres. Similar resolution was demonstrated for microfibers in the direction perpendicular to the fiber axis with hundreds of times larger field-of-view in comparison to microspheres. Using numerical solution of Maxwell's equations, it is shown that extraordinary close point objects can be resolved in the far field, if they oscillate out of phase. Possible super-resolution using resonant excitation of whispering gallery modes is also studied. Keywords: Optical super-resolution; near-field microscopy; confocal microscopy |
| first_indexed | 2024-09-23T13:36:02Z |
| format | Article |
| id | mit-1721.1/115076 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T13:36:02Z |
| publishDate | 2018 |
| publisher | SPIE |
| record_format | dspace |
| spelling | mit-1721.1/1150762022-09-28T14:55:37Z Fundamental limits of super-resolution microscopy by dielectric microspheres and microfibers Astratov, V. N. Maslov, A. V. Allen, K. W. Farahi, N. Li, Y. Brettin, A. Limberopoulos, N. I. Walker, D. E. Urbas, A. M. Liberman, Vladimir Rothschild, Mordechai Lincoln Laboratory Liberman, Vladimir Rothschild, Mordechai In recent years, optical super-resolution by microspheres and microfibers emerged as a new paradigm in nanoscale label-free and fluorescence imaging. However, the mechanisms of such imaging are still not completely understood and the resolution values are debated. In this work, the fundamental limits of super-resolution imaging by high-index barium-titanate microspheres and silica microfibers are studied using nanoplasmonic arrays made from Au and Al. A rigorous resolution analysis is developed based on the object's convolution with the point-spread function that has width well below the conventional (∼λ/2) diffraction limit, where λ is the illumination wavelength. A resolution of ∼λ/6-λ/7 is demonstrated for imaging nanoplasmonic arrays by microspheres. Similar resolution was demonstrated for microfibers in the direction perpendicular to the fiber axis with hundreds of times larger field-of-view in comparison to microspheres. Using numerical solution of Maxwell's equations, it is shown that extraordinary close point objects can be resolved in the far field, if they oscillate out of phase. Possible super-resolution using resonant excitation of whispering gallery modes is also studied. Keywords: Optical super-resolution; near-field microscopy; confocal microscopy 2018-04-30T13:10:18Z 2018-04-30T13:10:18Z 2016-04 2016-02 2018-03-30T15:43:38Z Article http://purl.org/eprint/type/ConferencePaper 0277-786X 1996-756X http://hdl.handle.net/1721.1/115076 Astratov, V. N., et al. “Fundamental Limits of Super-Resolution Microscopy by Dielectric Microspheres and Microfibers.” Edited by Alexander N. Cartwright, Dan V. Nicolau, and Dror Fixler. Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII, February 2016, San Francisco, California, USA, SPIE, April 2016 © 2016 SPIE http://dx.doi.org/10.1117/12.2212762 Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf SPIE SPIE |
| spellingShingle | Astratov, V. N. Maslov, A. V. Allen, K. W. Farahi, N. Li, Y. Brettin, A. Limberopoulos, N. I. Walker, D. E. Urbas, A. M. Liberman, Vladimir Rothschild, Mordechai Fundamental limits of super-resolution microscopy by dielectric microspheres and microfibers |
| title | Fundamental limits of super-resolution microscopy by dielectric microspheres and microfibers |
| title_full | Fundamental limits of super-resolution microscopy by dielectric microspheres and microfibers |
| title_fullStr | Fundamental limits of super-resolution microscopy by dielectric microspheres and microfibers |
| title_full_unstemmed | Fundamental limits of super-resolution microscopy by dielectric microspheres and microfibers |
| title_short | Fundamental limits of super-resolution microscopy by dielectric microspheres and microfibers |
| title_sort | fundamental limits of super resolution microscopy by dielectric microspheres and microfibers |
| url | http://hdl.handle.net/1721.1/115076 |
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