Rapid histopathological imaging of skin and breast cancer surgical specimens using immersion microscopy with ultraviolet surface excitation

Rapid histopathological evaluation of fresh, unfixed human tissue using optical sectioning microscopy would have applications to intraoperative surgical margin assessment. Microscopy with ultraviolet surface excitation (MUSE) is a low-cost optical sectioning technique using ultraviolet illumination...

Full description

Bibliographic Details
Main Authors: Quintana, Liza M., Vardeh, Hilde, Faulkner-Jones, Beverly E., Connolly, James L., Do, Daihung, Yoshitake, Tadayuki, Giacomelli, Michael, Cahill, Lucas Christopher, Fujimoto, James G
Other Authors: Harvard University--MIT Division of Health Sciences and Technology
Format: Article
Published: Nature Publishing Group 2018
Online Access:http://hdl.handle.net/1721.1/115185
https://orcid.org/0000-0003-4156-6484
https://orcid.org/0000-0002-2570-0770
https://orcid.org/0000-0003-3425-9486
https://orcid.org/0000-0002-0828-4357
_version_ 1826198525896032256
author Quintana, Liza M.
Vardeh, Hilde
Faulkner-Jones, Beverly E.
Connolly, James L.
Do, Daihung
Yoshitake, Tadayuki
Giacomelli, Michael
Cahill, Lucas Christopher
Fujimoto, James G
author2 Harvard University--MIT Division of Health Sciences and Technology
author_facet Harvard University--MIT Division of Health Sciences and Technology
Quintana, Liza M.
Vardeh, Hilde
Faulkner-Jones, Beverly E.
Connolly, James L.
Do, Daihung
Yoshitake, Tadayuki
Giacomelli, Michael
Cahill, Lucas Christopher
Fujimoto, James G
author_sort Quintana, Liza M.
collection MIT
description Rapid histopathological evaluation of fresh, unfixed human tissue using optical sectioning microscopy would have applications to intraoperative surgical margin assessment. Microscopy with ultraviolet surface excitation (MUSE) is a low-cost optical sectioning technique using ultraviolet illumination which limits fluorescence excitation to the specimen surface. In this paper, we characterize MUSE using high incident angle, water immersion illumination to improve sectioning. Propidium iodide is used as a nuclear stain and eosin yellow as a counterstain. Histologic features of specimens using MUSE, nonlinear microscopy (NLM) and conventional hematoxylin and eosin (H & E) histology were evaluated by pathologists to assess potential application in Mohs surgery for skin cancer and lumpectomy for breast cancer. MUSE images of basal cell carcinoma showed high correspondence with frozen section H & E histology, suggesting that MUSE may be applicable to Mohs surgery. However, correspondence in breast tissue between MUSE and paraffin embedded H & E histology was limited due to the thicker optical sectioning in MUSE, suggesting that further development is needed for breast surgical applicat ions. We further demonstrate that the transverse image resolution of MUSE is limited by the optical sectioning thickness and use co-registered NLM to quantify the improvement in MUSE optical sectioning from high incident angle water immersion illumination.
first_indexed 2024-09-23T11:06:14Z
format Article
id mit-1721.1/115185
institution Massachusetts Institute of Technology
last_indexed 2024-09-23T11:06:14Z
publishDate 2018
publisher Nature Publishing Group
record_format dspace
spelling mit-1721.1/1151852022-10-01T01:15:43Z Rapid histopathological imaging of skin and breast cancer surgical specimens using immersion microscopy with ultraviolet surface excitation Quintana, Liza M. Vardeh, Hilde Faulkner-Jones, Beverly E. Connolly, James L. Do, Daihung Yoshitake, Tadayuki Giacomelli, Michael Cahill, Lucas Christopher Fujimoto, James G Harvard University--MIT Division of Health Sciences and Technology Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Massachusetts Institute of Technology. Research Laboratory of Electronics Yoshitake, Tadayuki Giacomelli, Michael Cahill, Lucas Christopher Fujimoto, James G Rapid histopathological evaluation of fresh, unfixed human tissue using optical sectioning microscopy would have applications to intraoperative surgical margin assessment. Microscopy with ultraviolet surface excitation (MUSE) is a low-cost optical sectioning technique using ultraviolet illumination which limits fluorescence excitation to the specimen surface. In this paper, we characterize MUSE using high incident angle, water immersion illumination to improve sectioning. Propidium iodide is used as a nuclear stain and eosin yellow as a counterstain. Histologic features of specimens using MUSE, nonlinear microscopy (NLM) and conventional hematoxylin and eosin (H & E) histology were evaluated by pathologists to assess potential application in Mohs surgery for skin cancer and lumpectomy for breast cancer. MUSE images of basal cell carcinoma showed high correspondence with frozen section H & E histology, suggesting that MUSE may be applicable to Mohs surgery. However, correspondence in breast tissue between MUSE and paraffin embedded H & E histology was limited due to the thicker optical sectioning in MUSE, suggesting that further development is needed for breast surgical applicat ions. We further demonstrate that the transverse image resolution of MUSE is limited by the optical sectioning thickness and use co-registered NLM to quantify the improvement in MUSE optical sectioning from high incident angle water immersion illumination. National Institutes of Health (U.S.) (Grant CA178636-04) National Institutes of Health (U.S.) (Grant CA075289-19) National Institutes of Health (U.S.) (Grant CA183400-02) United States. Air Force Office of Scientific Research (Grant FA9550-12-1-0551) United States. Air Force Office of Scientific Research (Grant FA9550-15-1-0473) 2018-05-02T18:26:05Z 2018-05-02T18:26:05Z 2018-03 2017-10 2018-04-27T17:41:09Z Article http://purl.org/eprint/type/JournalArticle 2045-2322 http://hdl.handle.net/1721.1/115185 Yoshitake, Tadayuki et al. “Rapid Histopathological Imaging of Skin and Breast Cancer Surgical Specimens Using Immersion Microscopy with Ultraviolet Surface Excitation.” Scientific Reports 8, 1 (March 2018): 4476 © 2018 The Author(s) https://orcid.org/0000-0003-4156-6484 https://orcid.org/0000-0002-2570-0770 https://orcid.org/0000-0003-3425-9486 https://orcid.org/0000-0002-0828-4357 http://dx.doi.org/10.1038/s41598-018-22264-2 Scientific Reports Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/ application/pdf Nature Publishing Group Scientific Reports
spellingShingle Quintana, Liza M.
Vardeh, Hilde
Faulkner-Jones, Beverly E.
Connolly, James L.
Do, Daihung
Yoshitake, Tadayuki
Giacomelli, Michael
Cahill, Lucas Christopher
Fujimoto, James G
Rapid histopathological imaging of skin and breast cancer surgical specimens using immersion microscopy with ultraviolet surface excitation
title Rapid histopathological imaging of skin and breast cancer surgical specimens using immersion microscopy with ultraviolet surface excitation
title_full Rapid histopathological imaging of skin and breast cancer surgical specimens using immersion microscopy with ultraviolet surface excitation
title_fullStr Rapid histopathological imaging of skin and breast cancer surgical specimens using immersion microscopy with ultraviolet surface excitation
title_full_unstemmed Rapid histopathological imaging of skin and breast cancer surgical specimens using immersion microscopy with ultraviolet surface excitation
title_short Rapid histopathological imaging of skin and breast cancer surgical specimens using immersion microscopy with ultraviolet surface excitation
title_sort rapid histopathological imaging of skin and breast cancer surgical specimens using immersion microscopy with ultraviolet surface excitation
url http://hdl.handle.net/1721.1/115185
https://orcid.org/0000-0003-4156-6484
https://orcid.org/0000-0002-2570-0770
https://orcid.org/0000-0003-3425-9486
https://orcid.org/0000-0002-0828-4357
work_keys_str_mv AT quintanalizam rapidhistopathologicalimagingofskinandbreastcancersurgicalspecimensusingimmersionmicroscopywithultravioletsurfaceexcitation
AT vardehhilde rapidhistopathologicalimagingofskinandbreastcancersurgicalspecimensusingimmersionmicroscopywithultravioletsurfaceexcitation
AT faulknerjonesbeverlye rapidhistopathologicalimagingofskinandbreastcancersurgicalspecimensusingimmersionmicroscopywithultravioletsurfaceexcitation
AT connollyjamesl rapidhistopathologicalimagingofskinandbreastcancersurgicalspecimensusingimmersionmicroscopywithultravioletsurfaceexcitation
AT dodaihung rapidhistopathologicalimagingofskinandbreastcancersurgicalspecimensusingimmersionmicroscopywithultravioletsurfaceexcitation
AT yoshitaketadayuki rapidhistopathologicalimagingofskinandbreastcancersurgicalspecimensusingimmersionmicroscopywithultravioletsurfaceexcitation
AT giacomellimichael rapidhistopathologicalimagingofskinandbreastcancersurgicalspecimensusingimmersionmicroscopywithultravioletsurfaceexcitation
AT cahilllucaschristopher rapidhistopathologicalimagingofskinandbreastcancersurgicalspecimensusingimmersionmicroscopywithultravioletsurfaceexcitation
AT fujimotojamesg rapidhistopathologicalimagingofskinandbreastcancersurgicalspecimensusingimmersionmicroscopywithultravioletsurfaceexcitation