Bioelectronic measurement and feedback control of molecules in living cells
We describe an electrochemical measurement technique that enables bioelectronic measurements of reporter proteins in living cells as an alternative to traditional optical fluorescence. Using electronically programmable microfluidics, the measurement is in turn used to control the concentration of an...
| Main Authors: | , , , |
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| Format: | Article |
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Nature Publishing Group
2018
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| Online Access: | http://hdl.handle.net/1721.1/115186 |
| _version_ | 1826190965494251520 |
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| author | Banerjee, Areen Sarpeshkar, Rahul Weaver, Isaac Thorsen, Todd A. |
| author2 | Lincoln Laboratory |
| author_facet | Lincoln Laboratory Banerjee, Areen Sarpeshkar, Rahul Weaver, Isaac Thorsen, Todd A. |
| author_sort | Banerjee, Areen |
| collection | MIT |
| description | We describe an electrochemical measurement technique that enables bioelectronic measurements of reporter proteins in living cells as an alternative to traditional optical fluorescence. Using electronically programmable microfluidics, the measurement is in turn used to control the concentration of an inducer input that regulates production of the protein from a genetic promoter. The resulting bioelectronic and microfluidic negative-feedback loop then serves to regulate the concentration of the protein in the cell. We show measurements wherein a user-programmable set-point precisely alters the protein concentration in the cell with feedback-loop parameters affecting the dynamics of the closed-loop response in a predictable fashion. Our work does not require expensive optical fluorescence measurement techniques that are prone to toxicity in chronic settings, sophisticated time-lapse microscopy, or bulky/expensive chemo-stat instrumentation for dynamic measurement and control of biomolecules in cells. Therefore, it may be useful in creating a: Cheap, portable, chronic, dynamic, and precise all-electronic alternative for measurement and control of molecules in living cells. |
| first_indexed | 2024-09-23T08:48:01Z |
| format | Article |
| id | mit-1721.1/115186 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T08:48:01Z |
| publishDate | 2018 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/1151862022-09-30T11:21:46Z Bioelectronic measurement and feedback control of molecules in living cells Banerjee, Areen Sarpeshkar, Rahul Weaver, Isaac Thorsen, Todd A. Lincoln Laboratory Weaver, Isaac Thorsen, Todd A. We describe an electrochemical measurement technique that enables bioelectronic measurements of reporter proteins in living cells as an alternative to traditional optical fluorescence. Using electronically programmable microfluidics, the measurement is in turn used to control the concentration of an inducer input that regulates production of the protein from a genetic promoter. The resulting bioelectronic and microfluidic negative-feedback loop then serves to regulate the concentration of the protein in the cell. We show measurements wherein a user-programmable set-point precisely alters the protein concentration in the cell with feedback-loop parameters affecting the dynamics of the closed-loop response in a predictable fashion. Our work does not require expensive optical fluorescence measurement techniques that are prone to toxicity in chronic settings, sophisticated time-lapse microscopy, or bulky/expensive chemo-stat instrumentation for dynamic measurement and control of biomolecules in cells. Therefore, it may be useful in creating a: Cheap, portable, chronic, dynamic, and precise all-electronic alternative for measurement and control of molecules in living cells. National Science Foundation (U.S.) (Grant CCF 1124247) National Science Foundation (U.S.) (Grant 1606406) 2018-05-02T18:34:11Z 2018-05-02T18:34:11Z 2017-10 2017-03 2018-04-27T16:36:24Z Article http://purl.org/eprint/type/JournalArticle 2045-2322 http://hdl.handle.net/1721.1/115186 Banerjee, Areen, Isaac Weaver, Todd Thorsen, and Rahul Sarpeshkar. “Bioelectronic Measurement and Feedback Control of Molecules in Living Cells.” Scientific Reports 7, 1 (October 2017): 12511 © 2017 The Author(s) http://dx.doi.org/10.1038/S41598-017-12655-2 Scientific Reports Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/ application/pdf Nature Publishing Group Scientific Reports |
| spellingShingle | Banerjee, Areen Sarpeshkar, Rahul Weaver, Isaac Thorsen, Todd A. Bioelectronic measurement and feedback control of molecules in living cells |
| title | Bioelectronic measurement and feedback control of molecules in living cells |
| title_full | Bioelectronic measurement and feedback control of molecules in living cells |
| title_fullStr | Bioelectronic measurement and feedback control of molecules in living cells |
| title_full_unstemmed | Bioelectronic measurement and feedback control of molecules in living cells |
| title_short | Bioelectronic measurement and feedback control of molecules in living cells |
| title_sort | bioelectronic measurement and feedback control of molecules in living cells |
| url | http://hdl.handle.net/1721.1/115186 |
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