PD-L1 is an activation-independent marker of brown adipocytes
Programmed death ligand 1 (PD-L1) is expressed on a number of immune and cancer cells, where it can downregulate antitumor immune responses. Its expression has been linked to metabolic changes in these cells. Here we develop a radiolabeled camelid single-domain antibody (anti-PD-L1 VHH) to track PD-...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
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Springer Nature
2018
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| Online Access: | http://hdl.handle.net/1721.1/115203 https://orcid.org/0000-0002-7029-7415 https://orcid.org/0000-0002-1090-6071 |
| _version_ | 1826200725568356352 |
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| author | Ingram, Jessica R. Dougan, Michael Rashidian, Mohammad Knoll, Marko Keliher, Edmund J. Garrett, Sarah Garforth, Scott Blomberg, Olga S. Espinosa, Camilo Bhan, Atul Almo, Steven C. Weissleder, Ralph Dougan, Stephanie K. Lodish, Harvey F Ploegh, Hidde |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Ingram, Jessica R. Dougan, Michael Rashidian, Mohammad Knoll, Marko Keliher, Edmund J. Garrett, Sarah Garforth, Scott Blomberg, Olga S. Espinosa, Camilo Bhan, Atul Almo, Steven C. Weissleder, Ralph Dougan, Stephanie K. Lodish, Harvey F Ploegh, Hidde |
| author_sort | Ingram, Jessica R. |
| collection | MIT |
| description | Programmed death ligand 1 (PD-L1) is expressed on a number of immune and cancer cells, where it can downregulate antitumor immune responses. Its expression has been linked to metabolic changes in these cells. Here we develop a radiolabeled camelid single-domain antibody (anti-PD-L1 VHH) to track PD-L1 expression by immuno-positron emission tomography (PET). PET-CT imaging shows a robust and specific PD-L1 signal in brown adipose tissue (BAT). We confirm expression of PD-L1 on brown adipocytes and demonstrate that signal intensity does not change in response to cold exposure or β-adrenergic activation. This is the first robust method of visualizing murine brown fat independent of its activation state. |
| first_indexed | 2024-09-23T11:40:32Z |
| format | Article |
| id | mit-1721.1/115203 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T11:40:32Z |
| publishDate | 2018 |
| publisher | Springer Nature |
| record_format | dspace |
| spelling | mit-1721.1/1152032022-10-01T05:13:47Z PD-L1 is an activation-independent marker of brown adipocytes Ingram, Jessica R. Dougan, Michael Rashidian, Mohammad Knoll, Marko Keliher, Edmund J. Garrett, Sarah Garforth, Scott Blomberg, Olga S. Espinosa, Camilo Bhan, Atul Almo, Steven C. Weissleder, Ralph Dougan, Stephanie K. Lodish, Harvey F Ploegh, Hidde Massachusetts Institute of Technology. Department of Biology Lodish, Harvey F Ploegh, Hidde Programmed death ligand 1 (PD-L1) is expressed on a number of immune and cancer cells, where it can downregulate antitumor immune responses. Its expression has been linked to metabolic changes in these cells. Here we develop a radiolabeled camelid single-domain antibody (anti-PD-L1 VHH) to track PD-L1 expression by immuno-positron emission tomography (PET). PET-CT imaging shows a robust and specific PD-L1 signal in brown adipose tissue (BAT). We confirm expression of PD-L1 on brown adipocytes and demonstrate that signal intensity does not change in response to cold exposure or β-adrenergic activation. This is the first robust method of visualizing murine brown fat independent of its activation state. National Institutes of Health (U.S.) (Grant 2R01 DK047618-28) National Institute of Diabetes and Digestive and Kidney Diseases (U.S.) (Grant 2R01 DK047618-28) 2018-05-02T20:48:40Z 2018-05-02T20:48:40Z 2017-09 2017-02 2018-04-27T14:53:02Z Article http://purl.org/eprint/type/JournalArticle 2041-1723 http://hdl.handle.net/1721.1/115203 Ingram, Jessica R. et al. “PD-L1 Is an Activation-Independent Marker of Brown Adipocytes.” Nature Communications 8, 1 (September 2017): 647 © 2017 The Author(s) https://orcid.org/0000-0002-7029-7415 https://orcid.org/0000-0002-1090-6071 http://dx.doi.org/10.1038/S41467-017-00799-8 Nature Communications Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/ application/pdf Springer Nature Nature Communications |
| spellingShingle | Ingram, Jessica R. Dougan, Michael Rashidian, Mohammad Knoll, Marko Keliher, Edmund J. Garrett, Sarah Garforth, Scott Blomberg, Olga S. Espinosa, Camilo Bhan, Atul Almo, Steven C. Weissleder, Ralph Dougan, Stephanie K. Lodish, Harvey F Ploegh, Hidde PD-L1 is an activation-independent marker of brown adipocytes |
| title | PD-L1 is an activation-independent marker of brown adipocytes |
| title_full | PD-L1 is an activation-independent marker of brown adipocytes |
| title_fullStr | PD-L1 is an activation-independent marker of brown adipocytes |
| title_full_unstemmed | PD-L1 is an activation-independent marker of brown adipocytes |
| title_short | PD-L1 is an activation-independent marker of brown adipocytes |
| title_sort | pd l1 is an activation independent marker of brown adipocytes |
| url | http://hdl.handle.net/1721.1/115203 https://orcid.org/0000-0002-7029-7415 https://orcid.org/0000-0002-1090-6071 |
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