Motion of Knots in DNA Stretched by Elongational Fields
Knots in DNA occur in biological systems, serve as a model system for polymer entanglement, and affect the efficacy of modern genomics technologies. We study the motion of complex knots in DNA by stretching molecules with a divergent electric field that provides an elongational force. We demonstrate...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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American Physical Society
2018
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| Online Access: | http://hdl.handle.net/1721.1/115296 https://orcid.org/0000-0001-8399-5995 https://orcid.org/0000-0003-2147-9172 |
| _version_ | 1826201550116093952 |
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| author | Klotz, Alexander Soh, Beatrice W Doyle, Patrick S |
| author2 | Massachusetts Institute of Technology. Department of Chemical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Chemical Engineering Klotz, Alexander Soh, Beatrice W Doyle, Patrick S |
| author_sort | Klotz, Alexander |
| collection | MIT |
| description | Knots in DNA occur in biological systems, serve as a model system for polymer entanglement, and affect the efficacy of modern genomics technologies. We study the motion of complex knots in DNA by stretching molecules with a divergent electric field that provides an elongational force. We demonstrate that the motion of knots is nonisotropic and driven towards the closest end of the molecule. We show for the first time experimentally that knots can go from a mobile to a jammed state by varying an applied strain rate, and that this jamming is reversible. We measure the mobility of knots as a function of strain rate, demonstrating the conditions under which knots can be driven towards the ends of the molecule and untied. |
| first_indexed | 2024-09-23T11:53:30Z |
| format | Article |
| id | mit-1721.1/115296 |
| institution | Massachusetts Institute of Technology |
| language | English |
| last_indexed | 2024-09-23T11:53:30Z |
| publishDate | 2018 |
| publisher | American Physical Society |
| record_format | dspace |
| spelling | mit-1721.1/1152962022-09-27T22:37:50Z Motion of Knots in DNA Stretched by Elongational Fields Klotz, Alexander Soh, Beatrice W Doyle, Patrick S Massachusetts Institute of Technology. Department of Chemical Engineering Klotz, Alexander Soh, Beatrice W Doyle, Patrick S Knots in DNA occur in biological systems, serve as a model system for polymer entanglement, and affect the efficacy of modern genomics technologies. We study the motion of complex knots in DNA by stretching molecules with a divergent electric field that provides an elongational force. We demonstrate that the motion of knots is nonisotropic and driven towards the closest end of the molecule. We show for the first time experimentally that knots can go from a mobile to a jammed state by varying an applied strain rate, and that this jamming is reversible. We measure the mobility of knots as a function of strain rate, demonstrating the conditions under which knots can be driven towards the ends of the molecule and untied. 2018-05-10T18:12:09Z 2018-05-10T18:12:09Z 2018-05 2018-02 2018-05-03T18:00:15Z Article http://purl.org/eprint/type/JournalArticle 0031-9007 1079-7114 http://hdl.handle.net/1721.1/115296 Klotz, Alexander R. et al. "Motion of Knots in DNA Stretched by Elongational Fields." Physical Review Letters 120, 18 (May 2018): 188003 © 2018 American Physical Society https://orcid.org/0000-0001-8399-5995 https://orcid.org/0000-0003-2147-9172 en http://dx.doi.org/10.1103/PhysRevLett.120.188003 Physical Review Letters Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. American Physical Society application/pdf American Physical Society American Physical Society |
| spellingShingle | Klotz, Alexander Soh, Beatrice W Doyle, Patrick S Motion of Knots in DNA Stretched by Elongational Fields |
| title | Motion of Knots in DNA Stretched by Elongational Fields |
| title_full | Motion of Knots in DNA Stretched by Elongational Fields |
| title_fullStr | Motion of Knots in DNA Stretched by Elongational Fields |
| title_full_unstemmed | Motion of Knots in DNA Stretched by Elongational Fields |
| title_short | Motion of Knots in DNA Stretched by Elongational Fields |
| title_sort | motion of knots in dna stretched by elongational fields |
| url | http://hdl.handle.net/1721.1/115296 https://orcid.org/0000-0001-8399-5995 https://orcid.org/0000-0003-2147-9172 |
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