Synthetic biology approaches for engineering diverse bacterial species
Thesis: Ph. D., Massachusetts Institute of Technology, Department of Biological Engineering, June 2016.
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| Other Authors: | |
| Format: | Thesis |
| Language: | eng |
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Massachusetts Institute of Technology
2018
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| Online Access: | http://hdl.handle.net/1721.1/115449 |
| _version_ | 1811089201938038784 |
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| author | Brophy, Jennifer Ann Noelani |
| author2 | Christopher A. Voigt and Alan D. Grossman. |
| author_facet | Christopher A. Voigt and Alan D. Grossman. Brophy, Jennifer Ann Noelani |
| author_sort | Brophy, Jennifer Ann Noelani |
| collection | MIT |
| description | Thesis: Ph. D., Massachusetts Institute of Technology, Department of Biological Engineering, June 2016. |
| first_indexed | 2024-09-23T14:15:27Z |
| format | Thesis |
| id | mit-1721.1/115449 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T14:15:27Z |
| publishDate | 2018 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/1154492019-04-10T17:28:24Z Synthetic biology approaches for engineering diverse bacterial species Brophy, Jennifer Ann Noelani Christopher A. Voigt and Alan D. Grossman. Massachusetts Institute of Technology. Department of Biological Engineering. Massachusetts Institute of Technology. Department of Biological Engineering. Biological Engineering. Thesis: Ph. D., Massachusetts Institute of Technology, Department of Biological Engineering, June 2016. Cataloged from PDF version of thesis. "May 2016." Includes bibliographical references (pages 113-134). When engineers control gene expression, cells can be re-programmed to create living therapeutics or materials by initiating expression of biosynthetic pathways in response to specific signals. In this thesis, two new genetic tools were developed to aid the construction of genetic circuits and facilitate their delivery to bacteria isolated from diverse environments. First, antisense transcription was explored as a new tool for tuning gene expression in Escherichia coli. Antisense transcription was found to reliably repress gene expression and was applied tune simple genetic circuits. Second, an integrative conjugative element from Bacillus subtilis, ICEBsJ, was engineered to deliver exogenous DNA to diverse strains of undomesticated Gram-positive bacteria. Engineered ICEBsI conjugation was demonstrated in twenty different bacterial strains, spanning sixteen species and five genera. To demonstrate ICE's utility in creating new probiotics, the element was used to deliver functional nitrogen fixation pathways (nif clusters) to bacteria isolated from agricultural soils. Collectively, the tools presented here in provide a platform for programing bacteria from diverse environments for advanced applications. by Jennifer Ann Noelani Brophy. Ph. D. 2018-05-17T19:06:55Z 2018-05-17T19:06:55Z 2016 Thesis http://hdl.handle.net/1721.1/115449 1035374704 eng MIT theses are protected by copyright. They may be viewed, downloaded, or printed from this source but further reproduction or distribution in any format is prohibited without written permission. http://dspace.mit.edu/handle/1721.1/7582 xi, 134 pages application/pdf Massachusetts Institute of Technology |
| spellingShingle | Biological Engineering. Brophy, Jennifer Ann Noelani Synthetic biology approaches for engineering diverse bacterial species |
| title | Synthetic biology approaches for engineering diverse bacterial species |
| title_full | Synthetic biology approaches for engineering diverse bacterial species |
| title_fullStr | Synthetic biology approaches for engineering diverse bacterial species |
| title_full_unstemmed | Synthetic biology approaches for engineering diverse bacterial species |
| title_short | Synthetic biology approaches for engineering diverse bacterial species |
| title_sort | synthetic biology approaches for engineering diverse bacterial species |
| topic | Biological Engineering. |
| url | http://hdl.handle.net/1721.1/115449 |
| work_keys_str_mv | AT brophyjenniferannnoelani syntheticbiologyapproachesforengineeringdiversebacterialspecies |