Utilizing viruses to probe the material process - structure - property relationship : controlling catalytic properties via protein engineering and nanoscale synthesis
Thesis: Ph. D., Massachusetts Institute of Technology, Department of Biological Engineering, 2018.
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| Format: | Thesis |
| Language: | eng |
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Massachusetts Institute of Technology
2018
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| Online Access: | http://hdl.handle.net/1721.1/115761 |
| _version_ | 1811079538584584192 |
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| author | Ohmura, Jacqueline (Jacqueline Frances) |
| author2 | Angela M. Belcher. |
| author_facet | Angela M. Belcher. Ohmura, Jacqueline (Jacqueline Frances) |
| author_sort | Ohmura, Jacqueline (Jacqueline Frances) |
| collection | MIT |
| description | Thesis: Ph. D., Massachusetts Institute of Technology, Department of Biological Engineering, 2018. |
| first_indexed | 2024-09-23T11:16:39Z |
| format | Thesis |
| id | mit-1721.1/115761 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T11:16:39Z |
| publishDate | 2018 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/1157612019-04-12T23:22:07Z Utilizing viruses to probe the material process - structure - property relationship : controlling catalytic properties via protein engineering and nanoscale synthesis Controlling catalytic properties via protein engineering and nanoscale synthesis Ohmura, Jacqueline (Jacqueline Frances) Angela M. Belcher. Massachusetts Institute of Technology. Department of Biological Engineering. Massachusetts Institute of Technology. Department of Biological Engineering. Biological Engineering. Thesis: Ph. D., Massachusetts Institute of Technology, Department of Biological Engineering, 2018. Cataloged from PDF version of thesis. Includes bibliographical references (pages 136-146). From the fabrication of fine chemicals, to the increasing attainability of a non-petrochemical based energy infrastructure, catalysts play an important role in meeting the increasing energy and consumable demands of today without compromising the global health of tomorrow. Development of these catalysts relies on the fundamental understanding of the effects individual catalyst properties have on catalytic function. Unfortunately, control, and therefore deconvolution of individual parameter effects, can be quite challenging. Due to the nanoscale formfactor and wide range of available surface chemistries, biological catalyst fabrication affords one solution to this challenge. To this end, this work details the processing of M13 bacteriophage as a synthetic toolbox to modulate key catalyst parameters to elucidate the relationship between catalyst structure and performance. With respect to electrocatalysis, a biotemplating method for the development of tunable 3D nanofoams is detailed. Viral templates were rationally assembled into a variety of genetically programmable architectures and subsequently templated into a variety of material compositions. Subsequently, this synthetic method was employed to examine the effects of nanostructure on electro-catalytic activity. Next, nanoparticle driven heterogeneous catalysis was targeted. Nanoparticle-protein binding affinities were leveraged to explore the relationship between nanoparticles and their supports to identify a selective, base free alcohol oxidation catalyst. Finally, the surface proteins of the M13 virus were modified to mirror homogeneous copper-ligand chemistries. These viruses displayed binding pocket free copper complexation and catalytic efficacy in addition to recyclability and solvent robustness. Subsequently, the multiple functional handles of the viron were utilized to create catalytic ensembles of varying ratios. Single and dendrimeric TEMPO (4-Carboxy-2,2,6,6-tetramethylpiperidine 1-oxyl) were chemically conjugated to the surface of several catalytically active phage clones further tailoring catalytic function. Taken together, these studies provide strong evidence of the utility of biologically fabricated materials for catalytic design. by Jacqueline Ohmura. Ph. D. 2018-05-23T16:33:38Z 2018-05-23T16:33:38Z 2018 2018 Thesis http://hdl.handle.net/1721.1/115761 1036987189 eng MIT theses are protected by copyright. They may be viewed, downloaded, or printed from this source but further reproduction or distribution in any format is prohibited without written permission. http://dspace.mit.edu/handle/1721.1/7582 153 pages application/pdf Massachusetts Institute of Technology |
| spellingShingle | Biological Engineering. Ohmura, Jacqueline (Jacqueline Frances) Utilizing viruses to probe the material process - structure - property relationship : controlling catalytic properties via protein engineering and nanoscale synthesis |
| title | Utilizing viruses to probe the material process - structure - property relationship : controlling catalytic properties via protein engineering and nanoscale synthesis |
| title_full | Utilizing viruses to probe the material process - structure - property relationship : controlling catalytic properties via protein engineering and nanoscale synthesis |
| title_fullStr | Utilizing viruses to probe the material process - structure - property relationship : controlling catalytic properties via protein engineering and nanoscale synthesis |
| title_full_unstemmed | Utilizing viruses to probe the material process - structure - property relationship : controlling catalytic properties via protein engineering and nanoscale synthesis |
| title_short | Utilizing viruses to probe the material process - structure - property relationship : controlling catalytic properties via protein engineering and nanoscale synthesis |
| title_sort | utilizing viruses to probe the material process structure property relationship controlling catalytic properties via protein engineering and nanoscale synthesis |
| topic | Biological Engineering. |
| url | http://hdl.handle.net/1721.1/115761 |
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