Microfluidic synthesis, characterization, and applications of bioinspired deformable microparticles
Thesis: Ph. D., Massachusetts Institute of Technology, Department of Biological Engineering, 2018.
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| Other Authors: | |
| Format: | Thesis |
| Language: | eng |
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Massachusetts Institute of Technology
2018
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| Online Access: | http://hdl.handle.net/1721.1/115762 |
| _version_ | 1811098331634466816 |
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| author | Chen, Lynna |
| author2 | Patrick S. Doyle. |
| author_facet | Patrick S. Doyle. Chen, Lynna |
| author_sort | Chen, Lynna |
| collection | MIT |
| description | Thesis: Ph. D., Massachusetts Institute of Technology, Department of Biological Engineering, 2018. |
| first_indexed | 2024-09-23T17:13:23Z |
| format | Thesis |
| id | mit-1721.1/115762 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T17:13:23Z |
| publishDate | 2018 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/1157622019-04-11T05:13:17Z Microfluidic synthesis, characterization, and applications of bioinspired deformable microparticles Chen, Lynna Patrick S. Doyle. Massachusetts Institute of Technology. Department of Biological Engineering. Massachusetts Institute of Technology. Department of Biological Engineering. Biological Engineering. Thesis: Ph. D., Massachusetts Institute of Technology, Department of Biological Engineering, 2018. Cataloged from PDF version of thesis. Includes bibliographical references (pages 135-148). Polymeric microparticles have a wide variety of uses, ranging from traditional applications in paints and coatings, to specialized applications in medical therapeutics and diagnostics. For biological applications - including drug delivery, analytical assays, and tissue engineering - it is important to tailor the interactions between the microparticles and their external environment. To do this, it is necessary to precisely control the physical and chemical properties of the engineered microparticles. Recently, it has become apparent that in addition to particle chemistry, the physical properties of a particle - for example, size, shape, internal structure, and mechanical deformability - play an important role in determining particle behaviour in biological environments. However, it remains largely unknown exactly how these various physical properties influence particle behaviour and function, and how these properties should be exploited for different applications. This thesis focuses on the development and characterization of polymeric hydrogel microparticles with well-controlled physical and chemical properties, and shows several applications of these custom microparticles. In particular, we explore particle motifs inspired by biological entities, designing particles with different shapes, internal structure, and mechanical deformability, functionalized with proteins and nucleic acids. We employ microfluidic tools for synthesis and characterization of these hydrogel microparticles, and also investigate the interaction of functionalized particles with nucleic acids and cells, in the context of biomolecule detection and specific cell capture, respectively. Based on the microfluidic particle synthesis technique, stop flow lithography, we fabricate custom particles - including non-spherical 3D capsules and 2D extruded cylindrical rings with systematically varied internal architecture. We design microfluidic channels to study the flow and deformation of these particles, investigating the effects of internal structure, size, and stiffness on passage through microfluidic constrictions. We expand on this work, designing a microfluidic platform to specifically position particles in hydrodynamic traps, based on particle physical properties. This platform enables subsequent encapsulation of immobilized particles in monodisperse, isolated aqueous droplets. We demonstrate the platform's utility with chemically functionalized microparticles enabling sensitive, multiplexed microRNA detection. To further explore the interactions of functionalized microparticles with biological systems, we study how antibody-functionalized microparticles of varying shape can capture specific cells for future diagnostic applications. by Lynna Chen. Ph. D. 2018-05-23T16:33:41Z 2018-05-23T16:33:41Z 2018 2018 Thesis http://hdl.handle.net/1721.1/115762 1036987269 eng MIT theses are protected by copyright. They may be viewed, downloaded, or printed from this source but further reproduction or distribution in any format is prohibited without written permission. http://dspace.mit.edu/handle/1721.1/7582 148 pages application/pdf Massachusetts Institute of Technology |
| spellingShingle | Biological Engineering. Chen, Lynna Microfluidic synthesis, characterization, and applications of bioinspired deformable microparticles |
| title | Microfluidic synthesis, characterization, and applications of bioinspired deformable microparticles |
| title_full | Microfluidic synthesis, characterization, and applications of bioinspired deformable microparticles |
| title_fullStr | Microfluidic synthesis, characterization, and applications of bioinspired deformable microparticles |
| title_full_unstemmed | Microfluidic synthesis, characterization, and applications of bioinspired deformable microparticles |
| title_short | Microfluidic synthesis, characterization, and applications of bioinspired deformable microparticles |
| title_sort | microfluidic synthesis characterization and applications of bioinspired deformable microparticles |
| topic | Biological Engineering. |
| url | http://hdl.handle.net/1721.1/115762 |
| work_keys_str_mv | AT chenlynna microfluidicsynthesischaracterizationandapplicationsofbioinspireddeformablemicroparticles |