Adiponectin Deficiency Promotes Tumor Growth in Mice by Reducing Macrophage Infiltration
Adiponectin is an adipocyte-derived plasma protein that has been implicated in regulating angiogenesis, but the role of adiponectin in regulating this process is still controversial. In this study, in order to determine whether adiponectin affects tumor growth and tumor induced vascularization, we i...
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| Format: | Article |
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Public Library of Science (PLoS)
2018
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| Online Access: | http://hdl.handle.net/1721.1/116200 https://orcid.org/0000-0002-7029-7415 |
| _version_ | 1826190773028126720 |
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| author | Sun, Yutong Lodish, Harvey F |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Sun, Yutong Lodish, Harvey F |
| author_sort | Sun, Yutong |
| collection | MIT |
| description | Adiponectin is an adipocyte-derived plasma protein that has been implicated in regulating angiogenesis, but the role of adiponectin in regulating this process is still controversial. In this study, in order to determine whether adiponectin affects tumor growth and tumor induced vascularization, we implanted B16F10 melanoma and Lewis Lung Carcinoma cells subcutaneously into adiponectin knockout and wild-type control mice, and found that adiponectin deficiency markedly promoted the growth of both tumors. Immunohistochemical analyses indicated that adiponectin deficiency reduced macrophage recruitment to the tumor, but did not affect cancer cell mitosis, apoptosis, or tumor-associated angiogenesis. In addition, treatment with recombinant adiponectin did not affect the proliferation of cultured B16F10 tumor cells. Importantly, the restoration of microphage infiltration at an early stage of tumorigenesis by means of co-injection of B16F10 cells and macrophages reversed the increased tumor growth in adiponectin knockout mice. Thus, we conclude that the enhanced tumor growth observed in adiponectin deficient mice is likely due to the reduction of macrophage infiltration rather than enhanced angiogenesis. |
| first_indexed | 2024-09-23T08:45:29Z |
| format | Article |
| id | mit-1721.1/116200 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T08:45:29Z |
| publishDate | 2018 |
| publisher | Public Library of Science (PLoS) |
| record_format | dspace |
| spelling | mit-1721.1/1162002022-09-23T14:21:26Z Adiponectin Deficiency Promotes Tumor Growth in Mice by Reducing Macrophage Infiltration Sun, Yutong Lodish, Harvey F Massachusetts Institute of Technology. Department of Biology Lodish, Harvey F Adiponectin is an adipocyte-derived plasma protein that has been implicated in regulating angiogenesis, but the role of adiponectin in regulating this process is still controversial. In this study, in order to determine whether adiponectin affects tumor growth and tumor induced vascularization, we implanted B16F10 melanoma and Lewis Lung Carcinoma cells subcutaneously into adiponectin knockout and wild-type control mice, and found that adiponectin deficiency markedly promoted the growth of both tumors. Immunohistochemical analyses indicated that adiponectin deficiency reduced macrophage recruitment to the tumor, but did not affect cancer cell mitosis, apoptosis, or tumor-associated angiogenesis. In addition, treatment with recombinant adiponectin did not affect the proliferation of cultured B16F10 tumor cells. Importantly, the restoration of microphage infiltration at an early stage of tumorigenesis by means of co-injection of B16F10 cells and macrophages reversed the increased tumor growth in adiponectin knockout mice. Thus, we conclude that the enhanced tumor growth observed in adiponectin deficient mice is likely due to the reduction of macrophage infiltration rather than enhanced angiogenesis. National Institute of Diabetes and Digestive and Kidney Diseases (U.S.) (Grant 5R01DK047618-22) 2018-06-11T15:07:33Z 2018-06-11T15:07:33Z 2010-08 2010-05 2018-06-08T17:41:05Z Article http://purl.org/eprint/type/JournalArticle 1932-6203 http://hdl.handle.net/1721.1/116200 Sun, Yutong, and Harvey F. Lodish. “Adiponectin Deficiency Promotes Tumor Growth in Mice by Reducing Macrophage Infiltration.” Edited by Samithamby Jeyaseelan. PLoS ONE 5, 8 (August 2010): e11987 © 2010 Sun, Lodish https://orcid.org/0000-0002-7029-7415 http://dx.doi.org/10.1371/journal.pone.0011987 PLoS ONE Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/ application/pdf Public Library of Science (PLoS) PLoS |
| spellingShingle | Sun, Yutong Lodish, Harvey F Adiponectin Deficiency Promotes Tumor Growth in Mice by Reducing Macrophage Infiltration |
| title | Adiponectin Deficiency Promotes Tumor Growth in Mice by Reducing Macrophage Infiltration |
| title_full | Adiponectin Deficiency Promotes Tumor Growth in Mice by Reducing Macrophage Infiltration |
| title_fullStr | Adiponectin Deficiency Promotes Tumor Growth in Mice by Reducing Macrophage Infiltration |
| title_full_unstemmed | Adiponectin Deficiency Promotes Tumor Growth in Mice by Reducing Macrophage Infiltration |
| title_short | Adiponectin Deficiency Promotes Tumor Growth in Mice by Reducing Macrophage Infiltration |
| title_sort | adiponectin deficiency promotes tumor growth in mice by reducing macrophage infiltration |
| url | http://hdl.handle.net/1721.1/116200 https://orcid.org/0000-0002-7029-7415 |
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