Measurement of red blood cell mechanics during morphological changes
The human red blood cell (RBC) membrane, a fluid lipid bilayer tethered to an elastic 2D spectrin network, provides the principal control of the cell's morphology and mechanics. These properties, in turn, influence the ability of RBCs to transport oxygen in circulation. Current mechanical measu...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
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National Academy of Sciences (U.S.)
2018
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| Online Access: | http://hdl.handle.net/1721.1/116448 |
| _version_ | 1811089361293279232 |
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| author | Best, C. A. Kuriabova, T. Henle, M. L. Levine, A. J. Park, YongKeun Badizadegan, Kamran Dasari, Ramachandra Rao Feld, Michael S Popescu, Gabriel |
| author2 | Massachusetts Institute of Technology. Department of Chemistry |
| author_facet | Massachusetts Institute of Technology. Department of Chemistry Best, C. A. Kuriabova, T. Henle, M. L. Levine, A. J. Park, YongKeun Badizadegan, Kamran Dasari, Ramachandra Rao Feld, Michael S Popescu, Gabriel |
| author_sort | Best, C. A. |
| collection | MIT |
| description | The human red blood cell (RBC) membrane, a fluid lipid bilayer tethered to an elastic 2D spectrin network, provides the principal control of the cell's morphology and mechanics. These properties, in turn, influence the ability of RBCs to transport oxygen in circulation. Current mechanical measurements of RBCs rely on external loads. Here we apply a noncontact optical interferometric technique to quantify the thermal fluctuations of RBC membranes with 3 nm accuracy over a broad range of spatial and temporal frequencies. Combining this technique with a new mathematical model describing RBC membrane undulations, we measure the mechanical changes of RBCs as they undergo a transition from the normal discoid shape to the abnormal echinocyte and spherical shapes. These measurements indicate that, coincident with this morphological transition, there is a significant increase in the membrane's shear, area, and bending moduli. This mechanical transition can alter cell circulation and impede oxygen delivery. Keywords: membrane dynamics; microbiology; quantitative phase imaging |
| first_indexed | 2024-09-23T14:17:57Z |
| format | Article |
| id | mit-1721.1/116448 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T14:17:57Z |
| publishDate | 2018 |
| publisher | National Academy of Sciences (U.S.) |
| record_format | dspace |
| spelling | mit-1721.1/1164482022-09-29T08:31:09Z Measurement of red blood cell mechanics during morphological changes Best, C. A. Kuriabova, T. Henle, M. L. Levine, A. J. Park, YongKeun Badizadegan, Kamran Dasari, Ramachandra Rao Feld, Michael S Popescu, Gabriel Massachusetts Institute of Technology. Department of Chemistry Massachusetts Institute of Technology. Spectroscopy Laboratory Park, YongKeun Badizadegan, Kamran Dasari, Ramachandra Rao Feld, Michael S Popescu, Gabriel The human red blood cell (RBC) membrane, a fluid lipid bilayer tethered to an elastic 2D spectrin network, provides the principal control of the cell's morphology and mechanics. These properties, in turn, influence the ability of RBCs to transport oxygen in circulation. Current mechanical measurements of RBCs rely on external loads. Here we apply a noncontact optical interferometric technique to quantify the thermal fluctuations of RBC membranes with 3 nm accuracy over a broad range of spatial and temporal frequencies. Combining this technique with a new mathematical model describing RBC membrane undulations, we measure the mechanical changes of RBCs as they undergo a transition from the normal discoid shape to the abnormal echinocyte and spherical shapes. These measurements indicate that, coincident with this morphological transition, there is a significant increase in the membrane's shear, area, and bending moduli. This mechanical transition can alter cell circulation and impede oxygen delivery. Keywords: membrane dynamics; microbiology; quantitative phase imaging National Institutes of Health (U.S.) (Grant P41- RR02594-18) National Science Foundation (U.S.) (Grant CAREER 08-46660) National Science Foundation (U.S.) (Grant DMR-0907212) 2018-06-20T14:14:46Z 2018-06-20T14:14:46Z 2018-06-20 2018-06-18T18:43:26Z Article http://purl.org/eprint/type/JournalArticle 0027-8424 1091-6490 http://hdl.handle.net/1721.1/116448 Park, Y. et al. “Measurement of Red Blood Cell Mechanics During Morphological Changes.” Proceedings of the National Academy of Sciences 107, 15 (March 2010): 6731–6736 © 2010 National Academy of Sciences http://dx.doi.org/10.1073/pnas.0909533107 Proceedings of the National Academy of Sciences Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf National Academy of Sciences (U.S.) PNAS |
| spellingShingle | Best, C. A. Kuriabova, T. Henle, M. L. Levine, A. J. Park, YongKeun Badizadegan, Kamran Dasari, Ramachandra Rao Feld, Michael S Popescu, Gabriel Measurement of red blood cell mechanics during morphological changes |
| title | Measurement of red blood cell mechanics during morphological changes |
| title_full | Measurement of red blood cell mechanics during morphological changes |
| title_fullStr | Measurement of red blood cell mechanics during morphological changes |
| title_full_unstemmed | Measurement of red blood cell mechanics during morphological changes |
| title_short | Measurement of red blood cell mechanics during morphological changes |
| title_sort | measurement of red blood cell mechanics during morphological changes |
| url | http://hdl.handle.net/1721.1/116448 |
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