Whole-Mount X-Gal Staining of Mouse Tissues
Although the development of improved mouse models, including conditional deletions, marks an exciting time in mouse genetics, it is important to characterize and validate these models. Cre reporter strains allow researchers to assess the recombinase expression profile and function in individual Cre...
| Main Authors: | , , |
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| Other Authors: | |
| Format: | Article |
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Cold Spring Harbor Laboratory Press
2018
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| Online Access: | http://hdl.handle.net/1721.1/116574 https://orcid.org/0000-0001-5785-8911 |
| _version_ | 1826198216721301504 |
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| author | Gierut, J. J. Haigis, K. M. Jacks, Tyler E. |
| author2 | Koch Institute for Integrative Cancer Research at MIT |
| author_facet | Koch Institute for Integrative Cancer Research at MIT Gierut, J. J. Haigis, K. M. Jacks, Tyler E. |
| author_sort | Gierut, J. J. |
| collection | MIT |
| description | Although the development of improved mouse models, including conditional deletions, marks an exciting time in mouse genetics, it is important to characterize and validate these models. Cre reporter strains allow researchers to assess the recombinase expression profile and function in individual Cre mouse lines. These strains are engineered to express a reporter gene (usually LacZ) following the removal of a floxed STOP cassette, thus marking cell lineages that can be targeted with a given Cre line. This protocol provides a detailed method for the histochemical detection of β-galactosidase activity in Cre mouse strains. |
| first_indexed | 2024-09-23T11:01:15Z |
| format | Article |
| id | mit-1721.1/116574 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T11:01:15Z |
| publishDate | 2018 |
| publisher | Cold Spring Harbor Laboratory Press |
| record_format | dspace |
| spelling | mit-1721.1/1165742022-10-01T00:35:47Z Whole-Mount X-Gal Staining of Mouse Tissues Gierut, J. J. Haigis, K. M. Jacks, Tyler E. Koch Institute for Integrative Cancer Research at MIT Jacks, Tyler E. Although the development of improved mouse models, including conditional deletions, marks an exciting time in mouse genetics, it is important to characterize and validate these models. Cre reporter strains allow researchers to assess the recombinase expression profile and function in individual Cre mouse lines. These strains are engineered to express a reporter gene (usually LacZ) following the removal of a floxed STOP cassette, thus marking cell lineages that can be targeted with a given Cre line. This protocol provides a detailed method for the histochemical detection of β-galactosidase activity in Cre mouse strains. 2018-06-25T18:17:27Z 2018-06-25T18:17:27Z 2014-04 2018-06-25T16:57:32Z Article http://purl.org/eprint/type/JournalArticle 1559-6095 1940-3402 http://hdl.handle.net/1721.1/116574 Gierut, J. J. et al. “Whole-Mount X-Gal Staining of Mouse Tissues.” Cold Spring Harbor Protocols 2014, 4 (April 2014): 417-419 © 2014 Cold Spring Harbor Laboratory Press https://orcid.org/0000-0001-5785-8911 http://dx.doi.org/10.1101/PDB.PROT073452 Cold Spring Harbor Protocols Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Cold Spring Harbor Laboratory Press PMC |
| spellingShingle | Gierut, J. J. Haigis, K. M. Jacks, Tyler E. Whole-Mount X-Gal Staining of Mouse Tissues |
| title | Whole-Mount X-Gal Staining of Mouse Tissues |
| title_full | Whole-Mount X-Gal Staining of Mouse Tissues |
| title_fullStr | Whole-Mount X-Gal Staining of Mouse Tissues |
| title_full_unstemmed | Whole-Mount X-Gal Staining of Mouse Tissues |
| title_short | Whole-Mount X-Gal Staining of Mouse Tissues |
| title_sort | whole mount x gal staining of mouse tissues |
| url | http://hdl.handle.net/1721.1/116574 https://orcid.org/0000-0001-5785-8911 |
| work_keys_str_mv | AT gierutjj wholemountxgalstainingofmousetissues AT haigiskm wholemountxgalstainingofmousetissues AT jackstylere wholemountxgalstainingofmousetissues |