In Vivo Delivery of Lenti-Cre or Adeno-Cre into Mice Using Intranasal Instillation
Lung cancer remains the leading cause of cancer deaths among both men and women, with a lower rate of survival than both breast and prostate cancer. Development of the Cre/lox system and improved mouse models have allowed researchers to gain a better understanding of human disease, including lung ca...
| Main Authors: | , , |
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| Other Authors: | |
| Format: | Article |
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Cold Spring Harbor Laboratory Press
2018
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| Online Access: | http://hdl.handle.net/1721.1/116577 https://orcid.org/0000-0001-5785-8911 |
| Summary: | Lung cancer remains the leading cause of cancer deaths among both men and women, with a lower rate of survival than both breast and prostate cancer. Development of the Cre/lox system and improved mouse models have allowed researchers to gain a better understanding of human disease, including lung cancer. Through the viral delivery of Cre, gene function in adult mice can be precisely studied at a specific developmental stage or in a specific cell/tissue type of choice. This protocol describes how to produce adenovirus-Cre precipitate. Using this adeno-Cre (or lentivirus-Cre), Cre can be expressed in mouse lungs. The virus is delivered by intranasal instillation. |
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