In Vivo Delivery of Lenti-Cre or Adeno-Cre into Mice Using Intranasal Instillation
Lung cancer remains the leading cause of cancer deaths among both men and women, with a lower rate of survival than both breast and prostate cancer. Development of the Cre/lox system and improved mouse models have allowed researchers to gain a better understanding of human disease, including lung ca...
| Main Authors: | , , |
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| Other Authors: | |
| Format: | Article |
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Cold Spring Harbor Laboratory Press
2018
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| Online Access: | http://hdl.handle.net/1721.1/116577 https://orcid.org/0000-0001-5785-8911 |
| _version_ | 1826209911273422848 |
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| author | Gierut, J. J. Haigis, K. M. Jacks, Tyler E. |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Gierut, J. J. Haigis, K. M. Jacks, Tyler E. |
| author_sort | Gierut, J. J. |
| collection | MIT |
| description | Lung cancer remains the leading cause of cancer deaths among both men and women, with a lower rate of survival than both breast and prostate cancer. Development of the Cre/lox system and improved mouse models have allowed researchers to gain a better understanding of human disease, including lung cancer. Through the viral delivery of Cre, gene function in adult mice can be precisely studied at a specific developmental stage or in a specific cell/tissue type of choice. This protocol describes how to produce adenovirus-Cre precipitate. Using this adeno-Cre (or lentivirus-Cre), Cre can be expressed in mouse lungs. The virus is delivered by intranasal instillation. |
| first_indexed | 2024-09-23T14:35:02Z |
| format | Article |
| id | mit-1721.1/116577 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T14:35:02Z |
| publishDate | 2018 |
| publisher | Cold Spring Harbor Laboratory Press |
| record_format | dspace |
| spelling | mit-1721.1/1165772022-10-01T21:46:24Z In Vivo Delivery of Lenti-Cre or Adeno-Cre into Mice Using Intranasal Instillation Gierut, J. J. Haigis, K. M. Jacks, Tyler E. Massachusetts Institute of Technology. Department of Biology Koch Institute for Integrative Cancer Research at MIT Jacks, Tyler E. Lung cancer remains the leading cause of cancer deaths among both men and women, with a lower rate of survival than both breast and prostate cancer. Development of the Cre/lox system and improved mouse models have allowed researchers to gain a better understanding of human disease, including lung cancer. Through the viral delivery of Cre, gene function in adult mice can be precisely studied at a specific developmental stage or in a specific cell/tissue type of choice. This protocol describes how to produce adenovirus-Cre precipitate. Using this adeno-Cre (or lentivirus-Cre), Cre can be expressed in mouse lungs. The virus is delivered by intranasal instillation. 2018-06-25T18:34:57Z 2018-06-25T18:34:57Z 2014-03 2018-06-25T17:15:34Z Article http://purl.org/eprint/type/JournalArticle 1559-6095 http://hdl.handle.net/1721.1/116577 Gierut, J. J. et al. “In Vivo Delivery of Lenti-Cre or Adeno-Cre into Mice Using Intranasal Instillation.” Cold Spring Harbor Protocols 2014, 3 (March 2014): 307-309 © 2014 Cold Spring Harbor Laboratory Press https://orcid.org/0000-0001-5785-8911 http://dx.doi.org/10.1101/PDB.PROT073445 Cold Spring Harbor Protocols Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Cold Spring Harbor Laboratory Press PMC |
| spellingShingle | Gierut, J. J. Haigis, K. M. Jacks, Tyler E. In Vivo Delivery of Lenti-Cre or Adeno-Cre into Mice Using Intranasal Instillation |
| title | In Vivo Delivery of Lenti-Cre or Adeno-Cre into Mice Using Intranasal Instillation |
| title_full | In Vivo Delivery of Lenti-Cre or Adeno-Cre into Mice Using Intranasal Instillation |
| title_fullStr | In Vivo Delivery of Lenti-Cre or Adeno-Cre into Mice Using Intranasal Instillation |
| title_full_unstemmed | In Vivo Delivery of Lenti-Cre or Adeno-Cre into Mice Using Intranasal Instillation |
| title_short | In Vivo Delivery of Lenti-Cre or Adeno-Cre into Mice Using Intranasal Instillation |
| title_sort | in vivo delivery of lenti cre or adeno cre into mice using intranasal instillation |
| url | http://hdl.handle.net/1721.1/116577 https://orcid.org/0000-0001-5785-8911 |
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