A Multiplexed Single-Cell CRISPR Screening Platform Enables Systematic Dissection of the Unfolded Protein Response

A Multiplexed Single-Cell CRISPR Screening Platform Enables Systematic Dissection of the Unfolded Protein Response

Functional genomics efforts face tradeoffs between number of perturbations examined and complexity of phenotypes measured. We bridge this gap with Perturb-seq, which combines droplet-based single-cell RNA-seq with a strategy for barcoding CRISPR-mediated perturbations, allowing many perturbations to...

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Bibliographic Details
Main Authors: Adamson, Britt, Norman, Thomas M., Jost, Marco, Cho, Min Y., Nuñez, James K., Chen, Yuwen, Villalta, Jacqueline E., Gilbert, Luke A., Horlbeck, Max A., Hein, Marco Y., Pak, Ryan A., Gray, Andrew N., Gross, Carol A., Parnas, Oren, Weissman, Jonathan S., Dixit, Atray C., Regev, Aviv
Other Authors: Institute for Medical Engineering and Science
Format: Article
Published: Elsevier 2018
Online Access:http://hdl.handle.net/1721.1/116762
https://orcid.org/0000-0003-2193-9001
https://orcid.org/0000-0001-8567-2049

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