Maturation of Polycistronic mRNAs by the Endoribonuclease RNase Y and Its Associated Y-Complex in Bacillus Subtilis
Endonucleolytic cleavage within polycistronic mRNAs can lead to differential stability, and thus discordant abundance, among cotranscribed genes. RNase Y, the major endonuclease for mRNA decay in Bacillus subtilis, was originally identified for its cleavage activity toward the cggR-gapA operon, an e...
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National Academy of Sciences (U.S.)
2018
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| Online Access: | http://hdl.handle.net/1721.1/116791 https://orcid.org/0000-0002-2462-9474 https://orcid.org/0000-0001-7036-8511 |
| _version_ | 1811088849728700416 |
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| author | Losick, Richard DeLoughery, Aaron Lalanne, Jean-Benoit Li, Gene-Wei |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Losick, Richard DeLoughery, Aaron Lalanne, Jean-Benoit Li, Gene-Wei |
| author_sort | Losick, Richard |
| collection | MIT |
| description | Endonucleolytic cleavage within polycistronic mRNAs can lead to differential stability, and thus discordant abundance, among cotranscribed genes. RNase Y, the major endonuclease for mRNA decay in Bacillus subtilis, was originally identified for its cleavage activity toward the cggR-gapA operon, an event that differentiates the synthesis of a glycolytic enzyme from its transcriptional regulator. A three-protein Y-complex (YlbF, YmcA, and YaaT) was recently identified as also being required for this cleavage in vivo, raising the possibility that it is an accessory factor acting to regulate RNase Y. However, whether the Y-complex is broadly required for RNase Y activity is unknown. Here, we used end-enrichment RNA sequencing (Rend-seq) to globally identify operon mRNAs that undergo maturation posttranscriptionally by RNase Y and the Y-complex. We found that the Y-complex is required for the majority of RNase Y-mediated mRNA maturation events and also affects riboswitch abundance in B. subtilis. In contrast, noncoding RNA maturation by RNase Y often does not require the Y-complex. Furthermore, deletion of RNase Y has more pleiotropic effects on the transcriptome and cell growth than deletions of the Y-complex. We propose that the Y-complex is a specificity factor for RNase Y, with evidence that its role is conserved in Staphylococcus aureus. |
| first_indexed | 2024-09-23T14:08:28Z |
| format | Article |
| id | mit-1721.1/116791 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T14:08:28Z |
| publishDate | 2018 |
| publisher | National Academy of Sciences (U.S.) |
| record_format | dspace |
| spelling | mit-1721.1/1167912022-10-01T19:28:46Z Maturation of Polycistronic mRNAs by the Endoribonuclease RNase Y and Its Associated Y-Complex in Bacillus Subtilis Losick, Richard DeLoughery, Aaron Lalanne, Jean-Benoit Li, Gene-Wei Massachusetts Institute of Technology. Department of Biology Massachusetts Institute of Technology. Department of Physics DeLoughery, Aaron Lalanne, Jean-Benoit Li, Gene-Wei Endonucleolytic cleavage within polycistronic mRNAs can lead to differential stability, and thus discordant abundance, among cotranscribed genes. RNase Y, the major endonuclease for mRNA decay in Bacillus subtilis, was originally identified for its cleavage activity toward the cggR-gapA operon, an event that differentiates the synthesis of a glycolytic enzyme from its transcriptional regulator. A three-protein Y-complex (YlbF, YmcA, and YaaT) was recently identified as also being required for this cleavage in vivo, raising the possibility that it is an accessory factor acting to regulate RNase Y. However, whether the Y-complex is broadly required for RNase Y activity is unknown. Here, we used end-enrichment RNA sequencing (Rend-seq) to globally identify operon mRNAs that undergo maturation posttranscriptionally by RNase Y and the Y-complex. We found that the Y-complex is required for the majority of RNase Y-mediated mRNA maturation events and also affects riboswitch abundance in B. subtilis. In contrast, noncoding RNA maturation by RNase Y often does not require the Y-complex. Furthermore, deletion of RNase Y has more pleiotropic effects on the transcriptome and cell growth than deletions of the Y-complex. We propose that the Y-complex is a specificity factor for RNase Y, with evidence that its role is conserved in Staphylococcus aureus. National Institutes of Health (U.S.) (Grant R00GM105913) National Institutes of Health (U.S.) (Grant R35GM124732) 2018-07-05T14:26:27Z 2018-07-05T14:26:27Z 2018-06 2018-06-29T12:52:30Z Article http://purl.org/eprint/type/JournalArticle 0027-8424 1091-6490 http://hdl.handle.net/1721.1/116791 DeLoughery, Aaron et al. “Maturation of Polycistronic mRNAs by the Endoribonuclease RNase Y and Its Associated Y-Complex in Bacillus Subtilis.” Proceedings of the National Academy of Sciences 115, 24 (May 2018): E5585–E5594 © 2018 National Academy of Sciences https://orcid.org/0000-0002-2462-9474 https://orcid.org/0000-0001-7036-8511 http://dx.doi.org/10.1073/pnas.1803283115 Proceedings of the National Academy of Sciences Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf National Academy of Sciences (U.S.) PNAS |
| spellingShingle | Losick, Richard DeLoughery, Aaron Lalanne, Jean-Benoit Li, Gene-Wei Maturation of Polycistronic mRNAs by the Endoribonuclease RNase Y and Its Associated Y-Complex in Bacillus Subtilis |
| title | Maturation of Polycistronic mRNAs by the Endoribonuclease RNase Y and Its Associated Y-Complex in Bacillus Subtilis |
| title_full | Maturation of Polycistronic mRNAs by the Endoribonuclease RNase Y and Its Associated Y-Complex in Bacillus Subtilis |
| title_fullStr | Maturation of Polycistronic mRNAs by the Endoribonuclease RNase Y and Its Associated Y-Complex in Bacillus Subtilis |
| title_full_unstemmed | Maturation of Polycistronic mRNAs by the Endoribonuclease RNase Y and Its Associated Y-Complex in Bacillus Subtilis |
| title_short | Maturation of Polycistronic mRNAs by the Endoribonuclease RNase Y and Its Associated Y-Complex in Bacillus Subtilis |
| title_sort | maturation of polycistronic mrnas by the endoribonuclease rnase y and its associated y complex in bacillus subtilis |
| url | http://hdl.handle.net/1721.1/116791 https://orcid.org/0000-0002-2462-9474 https://orcid.org/0000-0001-7036-8511 |
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