The Structure Inventory of the Nuclear Pore Complex
The nuclear pore complex (NPC) is the principal gateway for molecular exchange between nucleus and cytoplasm across the nuclear envelope. Due to its sheer size of estimated 50-112 MDa and its complex buildup from about 500-1000 individual proteins, it is a difficult object to study for structural bi...
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| Format: | Article |
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Elsevier
2018
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| Online Access: | http://hdl.handle.net/1721.1/116855 https://orcid.org/0000-0001-8012-1512 |
| _version_ | 1811075897622528000 |
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| author | Schwartz, Thomas |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Schwartz, Thomas |
| author_sort | Schwartz, Thomas |
| collection | MIT |
| description | The nuclear pore complex (NPC) is the principal gateway for molecular exchange between nucleus and cytoplasm across the nuclear envelope. Due to its sheer size of estimated 50-112 MDa and its complex buildup from about 500-1000 individual proteins, it is a difficult object to study for structural biologists. Here, I review the extensive ensemble of high-resolution structures of the building blocks of the NPC. Concurrent with the increase in size and complexity, these latest, large structures and assemblies can now be used as the basis for hybrid approaches, primarily in combination with cryo-electron microscopic analysis, generating the first structure-based assembly models of the NPC. Going forward, the structures will be critically important for a detailed analysis of the NPC, including function, evolution, and assembly. Keywords: macromolecular complexes; nuclear pore complex |
| first_indexed | 2024-09-23T10:13:31Z |
| format | Article |
| id | mit-1721.1/116855 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T10:13:31Z |
| publishDate | 2018 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | mit-1721.1/1168552022-09-26T16:35:13Z The Structure Inventory of the Nuclear Pore Complex Schwartz, Thomas Massachusetts Institute of Technology. Department of Biology Schwartz, Thomas The nuclear pore complex (NPC) is the principal gateway for molecular exchange between nucleus and cytoplasm across the nuclear envelope. Due to its sheer size of estimated 50-112 MDa and its complex buildup from about 500-1000 individual proteins, it is a difficult object to study for structural biologists. Here, I review the extensive ensemble of high-resolution structures of the building blocks of the NPC. Concurrent with the increase in size and complexity, these latest, large structures and assemblies can now be used as the basis for hybrid approaches, primarily in combination with cryo-electron microscopic analysis, generating the first structure-based assembly models of the NPC. Going forward, the structures will be critically important for a detailed analysis of the NPC, including function, evolution, and assembly. Keywords: macromolecular complexes; nuclear pore complex National Institutes of Health (U.S.) (Grant GM077537) 2018-07-09T14:47:04Z 2018-07-09T14:47:04Z 2016-03 2016-03 2018-07-09T13:53:14Z Article http://purl.org/eprint/type/JournalArticle 0022-2836 1089-8638 http://hdl.handle.net/1721.1/116855 Schwartz, Thomas U. “The Structure Inventory of the Nuclear Pore Complex.” Journal of Molecular Biology 428, 10 (May 2016): 1986–2000 © 2016 Elsevier Ltd https://orcid.org/0000-0001-8012-1512 http://dx.doi.org/10.1016/J.JMB.2016.03.015 Journal of Molecular Biology Creative Commons Attribution-NonCommercial-NoDerivs License http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Elsevier PMC |
| spellingShingle | Schwartz, Thomas The Structure Inventory of the Nuclear Pore Complex |
| title | The Structure Inventory of the Nuclear Pore Complex |
| title_full | The Structure Inventory of the Nuclear Pore Complex |
| title_fullStr | The Structure Inventory of the Nuclear Pore Complex |
| title_full_unstemmed | The Structure Inventory of the Nuclear Pore Complex |
| title_short | The Structure Inventory of the Nuclear Pore Complex |
| title_sort | structure inventory of the nuclear pore complex |
| url | http://hdl.handle.net/1721.1/116855 https://orcid.org/0000-0001-8012-1512 |
| work_keys_str_mv | AT schwartzthomas thestructureinventoryofthenuclearporecomplex AT schwartzthomas structureinventoryofthenuclearporecomplex |