Quantifying argonaute proteins in and out of GW/P-bodies: Implications in microRNA activities
MicroRNAs (miRNAs) are a class of ∼22nt non-coding RNAs that regulate the translational potential and stability of mRNAs. Though constituting only 1-4% of human genes, miRNAs are predicted to regulate more than 60% of all mRNAs. The action of miRNAs is mediated through their associations with Argona...
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| Format: | Article |
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Springer
2018
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| Online Access: | http://hdl.handle.net/1721.1/116868 |
| _version_ | 1811096061318529024 |
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| author | Leung, Anthony K. L. Sharp, Phillip A. |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Leung, Anthony K. L. Sharp, Phillip A. |
| author_sort | Leung, Anthony K. L. |
| collection | MIT |
| description | MicroRNAs (miRNAs) are a class of ∼22nt non-coding RNAs that regulate the translational potential and stability of mRNAs. Though constituting only 1-4% of human genes, miRNAs are predicted to regulate more than 60% of all mRNAs. The action of miRNAs is mediated through their associations with Argonaute proteins and mRNA targets. Previous studies indicated that though the majority of Argonaute proteins is diffusely distributed in the cytoplasm, a small fraction is consistently observed to be concentrated in a cytoplasmic compartment called GW/P-bodies. In this chapter, we will provide a quantitative and dynamic view of the subcellular localization of miRNA function, followed by a discussion on the possible roles of PBs in miRNA silencing. |
| first_indexed | 2024-09-23T16:37:44Z |
| format | Article |
| id | mit-1721.1/116868 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T16:37:44Z |
| publishDate | 2018 |
| publisher | Springer |
| record_format | dspace |
| spelling | mit-1721.1/1168682022-09-29T20:27:18Z Quantifying argonaute proteins in and out of GW/P-bodies: Implications in microRNA activities Leung, Anthony K. L. Sharp, Phillip A. Massachusetts Institute of Technology. Department of Biology Koch Institute for Integrative Cancer Research at MIT Sharp, Phillip A. MicroRNAs (miRNAs) are a class of ∼22nt non-coding RNAs that regulate the translational potential and stability of mRNAs. Though constituting only 1-4% of human genes, miRNAs are predicted to regulate more than 60% of all mRNAs. The action of miRNAs is mediated through their associations with Argonaute proteins and mRNA targets. Previous studies indicated that though the majority of Argonaute proteins is diffusely distributed in the cytoplasm, a small fraction is consistently observed to be concentrated in a cytoplasmic compartment called GW/P-bodies. In this chapter, we will provide a quantitative and dynamic view of the subcellular localization of miRNA function, followed by a discussion on the possible roles of PBs in miRNA silencing. National Institutes of Health (U.S.) (Grant R01-CA133404) National Cancer Institute (U.S.) (Grant P01-CA42063) National Cancer Institute (U.S.) (Grant P30-CA14051) 2018-07-10T15:24:40Z 2018-07-10T15:24:40Z 2013 2018-07-09T16:37:16Z Article http://purl.org/eprint/type/JournalArticle 978-1-4614-5106-8 978-1-4614-5107-5 0065-2598 http://hdl.handle.net/1721.1/116868 Leung, Anthony K. L. and Phillip A. Sharp. "Quantifying argonaute proteins in and out of GW/P-bodies: Implications in microRNA activities." Advances in Experimental Medicine and Biology: Ten Years of Progress in GW/P Body Research, edited by E. Chan and M. Fritzler, Springer, 2013: 165-182 © Springer Science+Business Media New York 2013. http://dx.doi.org/10.1007/978-1-4614-5107-5_10 Advances in Experimental Medicine and Biology: Ten Years of Progress in GW/P Body Research Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Springer PMC |
| spellingShingle | Leung, Anthony K. L. Sharp, Phillip A. Quantifying argonaute proteins in and out of GW/P-bodies: Implications in microRNA activities |
| title | Quantifying argonaute proteins in and out of GW/P-bodies: Implications in microRNA activities |
| title_full | Quantifying argonaute proteins in and out of GW/P-bodies: Implications in microRNA activities |
| title_fullStr | Quantifying argonaute proteins in and out of GW/P-bodies: Implications in microRNA activities |
| title_full_unstemmed | Quantifying argonaute proteins in and out of GW/P-bodies: Implications in microRNA activities |
| title_short | Quantifying argonaute proteins in and out of GW/P-bodies: Implications in microRNA activities |
| title_sort | quantifying argonaute proteins in and out of gw p bodies implications in microrna activities |
| url | http://hdl.handle.net/1721.1/116868 |
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