CNS disease models with human pluripotent stem cells in the CRISPR age
In vitro differentiation of human pluripotent stem cells provides a systematic platform to investigate the physiological development and function of the human nervous system, as well as the etiology and consequence when these processes go awry. Recent development in three-dimensional (3D) organotypi...
| Main Authors: | , , |
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| Format: | Article |
| Language: | en_US |
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Elsevier
2018
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| Online Access: | http://hdl.handle.net/1721.1/117128 |
| _version_ | 1826213028299800576 |
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| author | Muffat, Julien Lin, Yun Jaenisch, Rudolf |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Muffat, Julien Lin, Yun Jaenisch, Rudolf |
| author_sort | Muffat, Julien |
| collection | MIT |
| description | In vitro differentiation of human pluripotent stem cells provides a systematic platform to investigate the physiological development and function of the human nervous system, as well as the etiology and consequence when these processes go awry. Recent development in three-dimensional (3D) organotypic culture systems allows modeling of the complex structure formation of the human CNS, and the intricate interactions between various resident neuronal and glial cell types. Combined with an ever-expanding genome editing and regulation toolkit such as CRISPR/Cas9, it is now a possibility to study human neurological disease in the relevant molecular, cellular and anatomical context. In this article, we review recent progress in 3D neural culture and the implications for disease modeling. |
| first_indexed | 2024-09-23T15:42:29Z |
| format | Article |
| id | mit-1721.1/117128 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T15:42:29Z |
| publishDate | 2018 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | mit-1721.1/1171282022-09-29T15:37:47Z CNS disease models with human pluripotent stem cells in the CRISPR age Muffat, Julien Lin, Yun Jaenisch, Rudolf Massachusetts Institute of Technology. Department of Biology Koch Institute for Integrative Cancer Research at MIT Jaenisch, R Lin, Yun Jaenisch, Rudolf In vitro differentiation of human pluripotent stem cells provides a systematic platform to investigate the physiological development and function of the human nervous system, as well as the etiology and consequence when these processes go awry. Recent development in three-dimensional (3D) organotypic culture systems allows modeling of the complex structure formation of the human CNS, and the intricate interactions between various resident neuronal and glial cell types. Combined with an ever-expanding genome editing and regulation toolkit such as CRISPR/Cas9, it is now a possibility to study human neurological disease in the relevant molecular, cellular and anatomical context. In this article, we review recent progress in 3D neural culture and the implications for disease modeling. National Institutes of Health (U.S.) (Grant HD045022) National Institutes of Health (U.S.) (Grant R37-CA084198) 2018-07-26T13:19:43Z 2018-07-26T13:19:43Z 2016-10 Article http://purl.org/eprint/type/JournalArticle 0955-0674 http://hdl.handle.net/1721.1/117128 Muffat, Julien et al. “CNS Disease Models with Human Pluripotent Stem Cells in the CRISPR Age.” Current Opinion in Cell Biology 43 (December 2016): 96–103 © 2016 Published by Elsevier Ltd en_US https://doi.org/10.1016/j.ceb.2016.10.001 Current Opinion in Cell Biology Creative Commons Attribution-NonCommercial-NoDerivs License http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Elsevier Prof. Jaenisch via Courtney Crummett |
| spellingShingle | Muffat, Julien Lin, Yun Jaenisch, Rudolf CNS disease models with human pluripotent stem cells in the CRISPR age |
| title | CNS disease models with human pluripotent stem cells in the CRISPR age |
| title_full | CNS disease models with human pluripotent stem cells in the CRISPR age |
| title_fullStr | CNS disease models with human pluripotent stem cells in the CRISPR age |
| title_full_unstemmed | CNS disease models with human pluripotent stem cells in the CRISPR age |
| title_short | CNS disease models with human pluripotent stem cells in the CRISPR age |
| title_sort | cns disease models with human pluripotent stem cells in the crispr age |
| url | http://hdl.handle.net/1721.1/117128 |
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