Viruses of the Nahant Collection, characterization of 251 marine Vibrionaceae viruses
Viruses are highly discriminating in their interactions with host cells and are thought to play a major role in maintaining diversity of environmental microbes. However, large-scale ecological and genomic studies of co-occurring virus-host pairs, required to characterize the mechanistic and genomic...
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| Format: | Article |
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Scientific Data
2018
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| Online Access: | http://hdl.handle.net/1721.1/117464 https://orcid.org/0000-0002-1997-947X https://orcid.org/0000-0002-1933-8103 https://orcid.org/0000-0001-5995-2936 https://orcid.org/0000-0001-9296-3733 |
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| author | Brown, Julia M. Kelly, Libusha Kauffman, Anne Kathryn Marie Sharma, Radhey Shyam VanInsberghe, David Stephen Elsherbini, Joseph Ahmed Polz, Martin F |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Brown, Julia M. Kelly, Libusha Kauffman, Anne Kathryn Marie Sharma, Radhey Shyam VanInsberghe, David Stephen Elsherbini, Joseph Ahmed Polz, Martin F |
| author_sort | Brown, Julia M. |
| collection | MIT |
| description | Viruses are highly discriminating in their interactions with host cells and are thought to play a major role in maintaining diversity of environmental microbes. However, large-scale ecological and genomic studies of co-occurring virus-host pairs, required to characterize the mechanistic and genomic foundations of virus-host interactions, are lacking. Here, we present the largest dataset of cultivated and sequenced co-occurring virus-host pairs that captures ecologically representative fine-scale diversity. Using the ubiquitous and ecologically diverse marine Vibrionaceae as a host platform, we isolate and sequence 251 dsDNA viruses and their hosts from three time points within a 93-day time-series study. The virus collection includes representatives of the three Caudovirales tailed virus morphotypes, a novel family of nontailed viruses, and the smallest (10,046 bp) and largest (348,911 bp) Vibrio virus genomes described. We provide general characterization and annotation of the viruses and describe read-mapping protocols to standardize genome presentation. The rich ecological and genomic contextualization of hosts and viruses make the Nahant Collection a unique platform for high-resolution studies of environmental virus-host infection networks. |
| first_indexed | 2024-09-23T14:26:00Z |
| format | Article |
| id | mit-1721.1/117464 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T14:26:00Z |
| publishDate | 2018 |
| publisher | Scientific Data |
| record_format | dspace |
| spelling | mit-1721.1/1174642022-09-29T09:27:09Z Viruses of the Nahant Collection, characterization of 251 marine Vibrionaceae viruses Brown, Julia M. Kelly, Libusha Kauffman, Anne Kathryn Marie Sharma, Radhey Shyam VanInsberghe, David Stephen Elsherbini, Joseph Ahmed Polz, Martin F Massachusetts Institute of Technology. Department of Biology Massachusetts Institute of Technology. Department of Civil and Environmental Engineering Kauffman, Anne Kathryn Marie Sharma, Radhey Shyam VanInsberghe, David Stephen Elsherbini, Joseph Ahmed Polz, Martin F Viruses are highly discriminating in their interactions with host cells and are thought to play a major role in maintaining diversity of environmental microbes. However, large-scale ecological and genomic studies of co-occurring virus-host pairs, required to characterize the mechanistic and genomic foundations of virus-host interactions, are lacking. Here, we present the largest dataset of cultivated and sequenced co-occurring virus-host pairs that captures ecologically representative fine-scale diversity. Using the ubiquitous and ecologically diverse marine Vibrionaceae as a host platform, we isolate and sequence 251 dsDNA viruses and their hosts from three time points within a 93-day time-series study. The virus collection includes representatives of the three Caudovirales tailed virus morphotypes, a novel family of nontailed viruses, and the smallest (10,046 bp) and largest (348,911 bp) Vibrio virus genomes described. We provide general characterization and annotation of the viruses and describe read-mapping protocols to standardize genome presentation. The rich ecological and genomic contextualization of hosts and viruses make the Nahant Collection a unique platform for high-resolution studies of environmental virus-host infection networks. National Science Foundation (U.S.) (OCE 1435993) 2018-08-22T14:38:33Z 2018-08-22T14:38:33Z 2018-07 2018-01 2018-08-21T13:29:25Z Article http://purl.org/eprint/type/JournalArticle 2052-4463 http://hdl.handle.net/1721.1/117464 Kauffman, Kathryn M., et al. “Viruses of the Nahant Collection, Characterization of 251 Marine Vibrionaceae Viruses.” Scientific Data, vol. 5, July 2018, p. 180114. © The Author(s) 2018. https://orcid.org/0000-0002-1997-947X https://orcid.org/0000-0002-1933-8103 https://orcid.org/0000-0001-5995-2936 https://orcid.org/0000-0001-9296-3733 http://dx.doi.org/10.1038/sdata.2018.114 Scientific Data Creative Commons Attribution 4.0 International License http://creativecommons.org/licenses/by/4.0/ application/pdf Scientific Data Nature |
| spellingShingle | Brown, Julia M. Kelly, Libusha Kauffman, Anne Kathryn Marie Sharma, Radhey Shyam VanInsberghe, David Stephen Elsherbini, Joseph Ahmed Polz, Martin F Viruses of the Nahant Collection, characterization of 251 marine Vibrionaceae viruses |
| title | Viruses of the Nahant Collection, characterization of 251 marine Vibrionaceae viruses |
| title_full | Viruses of the Nahant Collection, characterization of 251 marine Vibrionaceae viruses |
| title_fullStr | Viruses of the Nahant Collection, characterization of 251 marine Vibrionaceae viruses |
| title_full_unstemmed | Viruses of the Nahant Collection, characterization of 251 marine Vibrionaceae viruses |
| title_short | Viruses of the Nahant Collection, characterization of 251 marine Vibrionaceae viruses |
| title_sort | viruses of the nahant collection characterization of 251 marine vibrionaceae viruses |
| url | http://hdl.handle.net/1721.1/117464 https://orcid.org/0000-0002-1997-947X https://orcid.org/0000-0002-1933-8103 https://orcid.org/0000-0001-5995-2936 https://orcid.org/0000-0001-9296-3733 |
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