Precise Cas9 targeting enables genomic mutation prevention

Precise Cas9 targeting enables genomic mutation prevention

Here, we present a generalized method of guide RNA “tuning” that enables Cas9 to discriminate between two target sites that differ by a single-nucleotide polymorphism. We employ our methodology to generate an in vivo mutation prevention system in which Cas9 actively restricts the occurrence of undes...

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Bibliographic Details
Main Authors: Chavez, Alejandro, Pruitt, Benjamin W., Tuttle, Marcelle, Cecchi, Ryan J., Winston, Jordan, Turczyk, Brian M., Tung, Michael, Collins, James J., Church, George M, Shapiro, Rebecca
Other Authors: Massachusetts Institute of Technology. Institute for Medical Engineering & Science
Format: Article
Published: National Academy of Sciences (U.S.) 2018
Online Access:http://hdl.handle.net/1721.1/117584
https://orcid.org/0000-0001-8167-7096
https://orcid.org/0000-0002-5560-8246

Internet

http://hdl.handle.net/1721.1/117584
https://orcid.org/0000-0001-8167-7096
https://orcid.org/0000-0002-5560-8246

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