Precise Cas9 targeting enables genomic mutation prevention
Here, we present a generalized method of guide RNA “tuning” that enables Cas9 to discriminate between two target sites that differ by a single-nucleotide polymorphism. We employ our methodology to generate an in vivo mutation prevention system in which Cas9 actively restricts the occurrence of undes...
| Main Authors: | , , , , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Published: |
National Academy of Sciences (U.S.)
2018
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| Online Access: | http://hdl.handle.net/1721.1/117584 https://orcid.org/0000-0001-8167-7096 https://orcid.org/0000-0002-5560-8246 |