PiFlow: A biocompatible low-cost programmable dynamic flow pumping system utilizing a Raspberry Pi Zero and commercial piezoelectric pumps
With the rise of research utilizing microphysiological systems (MPSs), the need for tools that enable the physiological mimicking of the relevant cellular environment is vital. The limited ability to reproduce crucial features of the microenvironment, such as surrounding fluid flow and dynamic chang...
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| Format: | Article |
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Elsevier Inc.
2018
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| Online Access: | http://hdl.handle.net/1721.1/117641 https://orcid.org/0000-0001-8851-1224 https://orcid.org/0000-0001-7890-7209 https://orcid.org/0000-0001-5358-5450 https://orcid.org/0000-0002-1801-5548 |
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| author | Kassis, Timothy Perez, Paola M. Yang, Chloe J. Soenksen Martinez, Luis Ruben Trumper, David L Griffith, Linda G |
| author2 | Massachusetts Institute of Technology. Biotechnology Process Engineering Center |
| author_facet | Massachusetts Institute of Technology. Biotechnology Process Engineering Center Kassis, Timothy Perez, Paola M. Yang, Chloe J. Soenksen Martinez, Luis Ruben Trumper, David L Griffith, Linda G |
| author_sort | Kassis, Timothy |
| collection | MIT |
| description | With the rise of research utilizing microphysiological systems (MPSs), the need for tools that enable the physiological mimicking of the relevant cellular environment is vital. The limited ability to reproduce crucial features of the microenvironment, such as surrounding fluid flow and dynamic changes in biochemical stimuli, severely limits the types of experiments that can be carried out. Current equipment to achieve this, such as syringe and peristaltic pumps, is expensive, large, difficult to program and has limited potential for scalability. Here, we present a new pumping platform that is open-source, low-cost, modular, scalable, fully-programmable and easy to assemble that can be incorporated into cell culture systems to better recapitulate physiological environments. By controlling two commercially available piezoelectric pumps using a Raspberry Pi Zero microcontroller, the system is capable of producing arbitrary dynamic flow profiles with reliable flow rates ranging from 1 to 3000 µL/min as specified by an easily programmable Python-based script. We validated the accuracy of the flow rates, the use of time-varying profiles, and the practicality of the system by creating repeatable dynamic concentration profiles using a 3D-printed static micromixer. |
| first_indexed | 2024-09-23T14:27:17Z |
| format | Article |
| id | mit-1721.1/117641 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T14:27:17Z |
| publishDate | 2018 |
| publisher | Elsevier Inc. |
| record_format | dspace |
| spelling | mit-1721.1/1176412022-09-29T09:31:07Z PiFlow: A biocompatible low-cost programmable dynamic flow pumping system utilizing a Raspberry Pi Zero and commercial piezoelectric pumps Kassis, Timothy Perez, Paola M. Yang, Chloe J. Soenksen Martinez, Luis Ruben Trumper, David L Griffith, Linda G Massachusetts Institute of Technology. Biotechnology Process Engineering Center Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Mechanical Engineering Massachusetts Institute of Technology. Research Laboratory of Electronics Kassis, Timothy Perez, Paola M. Yang, Chloe J. Soenksen Martinez, Luis Ruben Trumper, David L Griffith, Linda G With the rise of research utilizing microphysiological systems (MPSs), the need for tools that enable the physiological mimicking of the relevant cellular environment is vital. The limited ability to reproduce crucial features of the microenvironment, such as surrounding fluid flow and dynamic changes in biochemical stimuli, severely limits the types of experiments that can be carried out. Current equipment to achieve this, such as syringe and peristaltic pumps, is expensive, large, difficult to program and has limited potential for scalability. Here, we present a new pumping platform that is open-source, low-cost, modular, scalable, fully-programmable and easy to assemble that can be incorporated into cell culture systems to better recapitulate physiological environments. By controlling two commercially available piezoelectric pumps using a Raspberry Pi Zero microcontroller, the system is capable of producing arbitrary dynamic flow profiles with reliable flow rates ranging from 1 to 3000 µL/min as specified by an easily programmable Python-based script. We validated the accuracy of the flow rates, the use of time-varying profiles, and the practicality of the system by creating repeatable dynamic concentration profiles using a 3D-printed static micromixer. United States. Defense Advanced Research Projects Agency. Microphysiological Systems Program (W911NF-12-2-0039) 2018-09-05T17:05:32Z 2018-09-05T17:05:32Z 2018-10 2018-06 2018-08-30T16:52:10Z Article http://purl.org/eprint/type/JournalArticle 24680672 http://hdl.handle.net/1721.1/117641 Kassis, Timothy, Paola M. Perez, Chloe J.W. Yang, Luis R. Soenksen, David L. Trumper, and Linda G. Griffith. “PiFlow: A Biocompatible Low-Cost Programmable Dynamic Flow Pumping System Utilizing a Raspberry Pi Zero and Commercial Piezoelectric Pumps.” HardwareX 4 (October 2018): e00034. https://orcid.org/0000-0001-8851-1224 https://orcid.org/0000-0001-7890-7209 https://orcid.org/0000-0001-5358-5450 https://orcid.org/0000-0002-1801-5548 http://dx.doi.org/10.1016/j.ohx.2018.e00034 HardwareX Creative Commons Attribution-NonCommercial-NoDerivs License http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Elsevier Inc. Elsevier |
| spellingShingle | Kassis, Timothy Perez, Paola M. Yang, Chloe J. Soenksen Martinez, Luis Ruben Trumper, David L Griffith, Linda G PiFlow: A biocompatible low-cost programmable dynamic flow pumping system utilizing a Raspberry Pi Zero and commercial piezoelectric pumps |
| title | PiFlow: A biocompatible low-cost programmable dynamic flow pumping system utilizing a Raspberry Pi Zero and commercial piezoelectric pumps |
| title_full | PiFlow: A biocompatible low-cost programmable dynamic flow pumping system utilizing a Raspberry Pi Zero and commercial piezoelectric pumps |
| title_fullStr | PiFlow: A biocompatible low-cost programmable dynamic flow pumping system utilizing a Raspberry Pi Zero and commercial piezoelectric pumps |
| title_full_unstemmed | PiFlow: A biocompatible low-cost programmable dynamic flow pumping system utilizing a Raspberry Pi Zero and commercial piezoelectric pumps |
| title_short | PiFlow: A biocompatible low-cost programmable dynamic flow pumping system utilizing a Raspberry Pi Zero and commercial piezoelectric pumps |
| title_sort | piflow a biocompatible low cost programmable dynamic flow pumping system utilizing a raspberry pi zero and commercial piezoelectric pumps |
| url | http://hdl.handle.net/1721.1/117641 https://orcid.org/0000-0001-8851-1224 https://orcid.org/0000-0001-7890-7209 https://orcid.org/0000-0001-5358-5450 https://orcid.org/0000-0002-1801-5548 |
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