DNA-Loaded Cationic Liposomes Efficiently Function as a Vaccine against Malarial Proteins
The delivery of antigens as DNA vaccines is an efficient alternative to induce immune responses against antigens, which are difficult to produce in recombinant form. However, the delivery of naked DNA is ineffective or relies on sophisticated ballistic devices. Here, we show a combination of liposom...
| Main Authors: | , , , , |
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| Format: | Article |
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Elsevier BV
2018
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| Online Access: | http://hdl.handle.net/1721.1/117733 |
| _version_ | 1826201684657831936 |
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| author | Fotoran, Wesley L. Santangelo, Rachele de Miranda, Beatriz N.M. Wunderlich, Gerhard Irvine, Darrell J |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Fotoran, Wesley L. Santangelo, Rachele de Miranda, Beatriz N.M. Wunderlich, Gerhard Irvine, Darrell J |
| author_sort | Fotoran, Wesley L. |
| collection | MIT |
| description | The delivery of antigens as DNA vaccines is an efficient alternative to induce immune responses against antigens, which are difficult to produce in recombinant form. However, the delivery of naked DNA is ineffective or relies on sophisticated ballistic devices. Here, we show a combination of liposome application and naked DNA vaccine that successfully overcomes these problems. Upon entrapment of plasmids encoding different antigens in cationic particles, transfection efficiencies similar to commercial kits were achieved in in vitro cell cultures. The liposome-based approach provided strong humoral responses against three malarial antigens, namely the Circumsporozoite protein and the C terminus of merozoite surface protein 1 from Plasmodium vivax (titers 104or 103–104, respectively) and P. falciparum Rhoptry antigen 5 from Plasmodium falciparum (titers 103–104). When employed in P. falciparum growth-inhibition assays, antibodies demonstrated consistent reinvasion-blocking activities that were dose dependent. Liposome-formulated DNA vaccines may prove useful when targets cannot be produced as recombinant proteins and when conformation-dependent and highly specific antibodies are mandatory. Keywords: cationic liposomes; DNA vaccine; malaria |
| first_indexed | 2024-09-23T11:55:23Z |
| format | Article |
| id | mit-1721.1/117733 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T11:55:23Z |
| publishDate | 2018 |
| publisher | Elsevier BV |
| record_format | dspace |
| spelling | mit-1721.1/1177332022-09-27T22:52:06Z DNA-Loaded Cationic Liposomes Efficiently Function as a Vaccine against Malarial Proteins Fotoran, Wesley L. Santangelo, Rachele de Miranda, Beatriz N.M. Wunderlich, Gerhard Irvine, Darrell J Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Materials Science and Engineering Koch Institute for Integrative Cancer Research at MIT Irvine, Darrell J The delivery of antigens as DNA vaccines is an efficient alternative to induce immune responses against antigens, which are difficult to produce in recombinant form. However, the delivery of naked DNA is ineffective or relies on sophisticated ballistic devices. Here, we show a combination of liposome application and naked DNA vaccine that successfully overcomes these problems. Upon entrapment of plasmids encoding different antigens in cationic particles, transfection efficiencies similar to commercial kits were achieved in in vitro cell cultures. The liposome-based approach provided strong humoral responses against three malarial antigens, namely the Circumsporozoite protein and the C terminus of merozoite surface protein 1 from Plasmodium vivax (titers 104or 103–104, respectively) and P. falciparum Rhoptry antigen 5 from Plasmodium falciparum (titers 103–104). When employed in P. falciparum growth-inhibition assays, antibodies demonstrated consistent reinvasion-blocking activities that were dose dependent. Liposome-formulated DNA vaccines may prove useful when targets cannot be produced as recombinant proteins and when conformation-dependent and highly specific antibodies are mandatory. Keywords: cationic liposomes; DNA vaccine; malaria 2018-09-12T20:46:33Z 2018-09-12T20:46:33Z 2017-08 2017-03 2018-09-06T18:28:27Z Article http://purl.org/eprint/type/JournalArticle 2329-0501 http://hdl.handle.net/1721.1/117733 Fotoran, Wesley L. et al. “DNA-Loaded Cationic Liposomes Efficiently Function as a Vaccine Against Malarial Proteins.” Molecular Therapy - Methods & Clinical Development 7 (December 2017): 1–10 © 2017 The Author(s) http://dx.doi.org/10.1016/J.OMTM.2017.08.004 Molecular Therapy - Methods & Clinical Development Creative Commons Attribution 4.0 International License http://creativecommons.org/licenses/by/4.0/ application/pdf Elsevier BV Elsevier |
| spellingShingle | Fotoran, Wesley L. Santangelo, Rachele de Miranda, Beatriz N.M. Wunderlich, Gerhard Irvine, Darrell J DNA-Loaded Cationic Liposomes Efficiently Function as a Vaccine against Malarial Proteins |
| title | DNA-Loaded Cationic Liposomes Efficiently Function as a Vaccine against Malarial Proteins |
| title_full | DNA-Loaded Cationic Liposomes Efficiently Function as a Vaccine against Malarial Proteins |
| title_fullStr | DNA-Loaded Cationic Liposomes Efficiently Function as a Vaccine against Malarial Proteins |
| title_full_unstemmed | DNA-Loaded Cationic Liposomes Efficiently Function as a Vaccine against Malarial Proteins |
| title_short | DNA-Loaded Cationic Liposomes Efficiently Function as a Vaccine against Malarial Proteins |
| title_sort | dna loaded cationic liposomes efficiently function as a vaccine against malarial proteins |
| url | http://hdl.handle.net/1721.1/117733 |
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