Label-free Neutrophil Enrichment from Patient-derived Airway Secretion Using Closed-loop Inertial Microfluidics
Airway secretions contain a large number of immune-related cells, e.g., neutrophils, macrophages, and lymphocytes, which can be used as a major resource to evaluate a variety of pulmonary diseases, both for research and clinical purposes. However, due to the heterogeneous and viscous nature of patie...
| Main Authors: | , , , , , |
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MyJoVE Corporation
2018
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| Online Access: | http://hdl.handle.net/1721.1/118444 https://orcid.org/0000-0003-3645-1930 https://orcid.org/0000-0003-0935-4158 https://orcid.org/0000-0001-7837-0448 https://orcid.org/0000-0001-7215-1439 |
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| author | Qu, Yanyan Lee, Janet S. Ryu, Hyunryul Choi, Kyungyong Kwon, Taehong Han, Jongyoon |
| author2 | Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science |
| author_facet | Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Qu, Yanyan Lee, Janet S. Ryu, Hyunryul Choi, Kyungyong Kwon, Taehong Han, Jongyoon |
| author_sort | Qu, Yanyan |
| collection | MIT |
| description | Airway secretions contain a large number of immune-related cells, e.g., neutrophils, macrophages, and lymphocytes, which can be used as a major resource to evaluate a variety of pulmonary diseases, both for research and clinical purposes. However, due to the heterogeneous and viscous nature of patient mucus, there is currently no reliable dissociation method that does not damage the host immune cells in the patient airway secretion. In this research, we introduce a sample preparation method that uses inertial microfluidics for the patient's immune assessment. Regardless of the heterogeneous fluidic properties of the clinical samples, the proposed method recovers more than 95% of neutrophils from airway secretion samples that are diluted 1,000-fold with milliliters of clean saline. By recirculating the concentrated output stream to the initial sample reservoir, a high concentration, recovery, and purity of the immune cells are provided; recirculation is considered a trade-off to the single-run syringe-based operation of inertial microfluidics. The closed-loop operation of spiral microfluidics provides leukocytes without physical or chemical disturbance, as demonstrated by the phorbol 12-myristate 13-acetate (PMA)-induced elastase release of sorted neutrophils. Keywords: Immunology and Infection, Issue 136, Inertial microfluidics, airway secretion, label-free cell sorting, heterogeneous biofluid, neutrophil enrichment, patient sample preparation |
| first_indexed | 2024-09-23T13:37:35Z |
| format | Article |
| id | mit-1721.1/118444 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T13:37:35Z |
| publishDate | 2018 |
| publisher | MyJoVE Corporation |
| record_format | dspace |
| spelling | mit-1721.1/1184442022-09-28T15:08:09Z Label-free Neutrophil Enrichment from Patient-derived Airway Secretion Using Closed-loop Inertial Microfluidics Qu, Yanyan Lee, Janet S. Ryu, Hyunryul Choi, Kyungyong Kwon, Taehong Han, Jongyoon Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Massachusetts Institute of Technology. Research Laboratory of Electronics Ryu, Hyunryul Choi, Kyungyong Kwon, Taehong Han, Jongyoon Airway secretions contain a large number of immune-related cells, e.g., neutrophils, macrophages, and lymphocytes, which can be used as a major resource to evaluate a variety of pulmonary diseases, both for research and clinical purposes. However, due to the heterogeneous and viscous nature of patient mucus, there is currently no reliable dissociation method that does not damage the host immune cells in the patient airway secretion. In this research, we introduce a sample preparation method that uses inertial microfluidics for the patient's immune assessment. Regardless of the heterogeneous fluidic properties of the clinical samples, the proposed method recovers more than 95% of neutrophils from airway secretion samples that are diluted 1,000-fold with milliliters of clean saline. By recirculating the concentrated output stream to the initial sample reservoir, a high concentration, recovery, and purity of the immune cells are provided; recirculation is considered a trade-off to the single-run syringe-based operation of inertial microfluidics. The closed-loop operation of spiral microfluidics provides leukocytes without physical or chemical disturbance, as demonstrated by the phorbol 12-myristate 13-acetate (PMA)-induced elastase release of sorted neutrophils. Keywords: Immunology and Infection, Issue 136, Inertial microfluidics, airway secretion, label-free cell sorting, heterogeneous biofluid, neutrophil enrichment, patient sample preparation National Institute of Allergy and Infectious Diseases (U.S.) (R21AI119042) National Institutes of Health (U.S.) (U24-AI118656) 2018-10-11T19:06:40Z 2018-10-11T19:06:40Z 2018-06 2018-10-10T13:24:36Z Article http://purl.org/eprint/type/JournalArticle 1940-087X http://hdl.handle.net/1721.1/118444 Ryu, Hyunryul, et al. “Label-Free Neutrophil Enrichment from Patient-Derived Airway Secretion Using Closed-Loop Inertial Microfluidics.” Journal of Visualized Experiments, no. 136, June 2018. © 2018 Journal of Visualized Experiments. https://orcid.org/0000-0003-3645-1930 https://orcid.org/0000-0003-0935-4158 https://orcid.org/0000-0001-7837-0448 https://orcid.org/0000-0001-7215-1439 http://dx.doi.org/10.3791/57673 Journal of Visualized Experiments Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf MyJoVE Corporation Journal of Visualized Experiments (JOVE) |
| spellingShingle | Qu, Yanyan Lee, Janet S. Ryu, Hyunryul Choi, Kyungyong Kwon, Taehong Han, Jongyoon Label-free Neutrophil Enrichment from Patient-derived Airway Secretion Using Closed-loop Inertial Microfluidics |
| title | Label-free Neutrophil Enrichment from Patient-derived Airway Secretion Using Closed-loop Inertial Microfluidics |
| title_full | Label-free Neutrophil Enrichment from Patient-derived Airway Secretion Using Closed-loop Inertial Microfluidics |
| title_fullStr | Label-free Neutrophil Enrichment from Patient-derived Airway Secretion Using Closed-loop Inertial Microfluidics |
| title_full_unstemmed | Label-free Neutrophil Enrichment from Patient-derived Airway Secretion Using Closed-loop Inertial Microfluidics |
| title_short | Label-free Neutrophil Enrichment from Patient-derived Airway Secretion Using Closed-loop Inertial Microfluidics |
| title_sort | label free neutrophil enrichment from patient derived airway secretion using closed loop inertial microfluidics |
| url | http://hdl.handle.net/1721.1/118444 https://orcid.org/0000-0003-3645-1930 https://orcid.org/0000-0003-0935-4158 https://orcid.org/0000-0001-7837-0448 https://orcid.org/0000-0001-7215-1439 |
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