Multidimensional solid-state NMR spectroscopy of plant cell walls
Plant biomass has become an important source of bio-renewable energy in modern society. The molecular structure of plant cell walls is difficult to characterize by most atomic-resolution techniques due to the insoluble and disordered nature of the cell wall. Solid-state NMR (SSNMR) spectroscopy is u...
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| Format: | Article |
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Elsevier BV
2018
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| Online Access: | http://hdl.handle.net/1721.1/118448 https://orcid.org/0000-0002-1801-924X https://orcid.org/0000-0001-5255-5858 |
| _version_ | 1811092720019570688 |
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| author | Wang, Tuo Phyo, Pyae Hong, Mei |
| author2 | Massachusetts Institute of Technology. Department of Chemistry |
| author_facet | Massachusetts Institute of Technology. Department of Chemistry Wang, Tuo Phyo, Pyae Hong, Mei |
| author_sort | Wang, Tuo |
| collection | MIT |
| description | Plant biomass has become an important source of bio-renewable energy in modern society. The molecular structure of plant cell walls is difficult to characterize by most atomic-resolution techniques due to the insoluble and disordered nature of the cell wall. Solid-state NMR (SSNMR) spectroscopy is uniquely suited for studying native hydrated plant cell walls at the molecular level with chemical resolution. Significant progress has been made in the last five years to elucidate the molecular structures and interactions of cellulose and matrix polysaccharides in plant cell walls. These studies have focused on primary cell walls of growing plants in both the dicotyledonous and grass families, as represented by the model plants Arabidopsis thaliana, Brachypodium distachyon, and Zea mays. To date, these SSNMR results have shown that 1) cellulose, hemicellulose, and pectins form a single network in the primary cell wall; 2) in dicot cell walls, the protein expansin targets the hemicellulose-enriched region of the cellulose microfibril for its wall-loosening function; and 3) primary wall cellulose has polymorphic structures that are distinct from the microbial cellulose structures. This article summarizes these key findings, and points out future directions of investigation to advance our fundamental understanding of plant cell wall structure and function. Keywords:
Cellulose; Matrix polysaccharide; Expansin; Lignin; Magic-angle spinning; Multidimensional correlation; Structural polymorphism; Cellulose-pectin interactions |
| first_indexed | 2024-09-23T15:23:13Z |
| format | Article |
| id | mit-1721.1/118448 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T15:23:13Z |
| publishDate | 2018 |
| publisher | Elsevier BV |
| record_format | dspace |
| spelling | mit-1721.1/1184482024-06-26T15:06:55Z Multidimensional solid-state NMR spectroscopy of plant cell walls Wang, Tuo Phyo, Pyae Hong, Mei Massachusetts Institute of Technology. Department of Chemistry Wang, Tuo Phyo, Pyae Hong, Mei Plant biomass has become an important source of bio-renewable energy in modern society. The molecular structure of plant cell walls is difficult to characterize by most atomic-resolution techniques due to the insoluble and disordered nature of the cell wall. Solid-state NMR (SSNMR) spectroscopy is uniquely suited for studying native hydrated plant cell walls at the molecular level with chemical resolution. Significant progress has been made in the last five years to elucidate the molecular structures and interactions of cellulose and matrix polysaccharides in plant cell walls. These studies have focused on primary cell walls of growing plants in both the dicotyledonous and grass families, as represented by the model plants Arabidopsis thaliana, Brachypodium distachyon, and Zea mays. To date, these SSNMR results have shown that 1) cellulose, hemicellulose, and pectins form a single network in the primary cell wall; 2) in dicot cell walls, the protein expansin targets the hemicellulose-enriched region of the cellulose microfibril for its wall-loosening function; and 3) primary wall cellulose has polymorphic structures that are distinct from the microbial cellulose structures. This article summarizes these key findings, and points out future directions of investigation to advance our fundamental understanding of plant cell wall structure and function. Keywords: Cellulose; Matrix polysaccharide; Expansin; Lignin; Magic-angle spinning; Multidimensional correlation; Structural polymorphism; Cellulose-pectin interactions 2018-10-11T19:46:50Z 2018-10-11T19:46:50Z 2016-08 2016-08 2018-09-25T17:55:59Z Article http://purl.org/eprint/type/JournalArticle 0926-2040 http://hdl.handle.net/1721.1/118448 Wang, Tuo et al. “Multidimensional Solid-State NMR Spectroscopy of Plant Cell Walls.” Solid State Nuclear Magnetic Resonance 78 (September 2016): 56–63 © 2016 Elsevier Inc https://orcid.org/0000-0002-1801-924X https://orcid.org/0000-0001-5255-5858 http://dx.doi.org/10.1016/J.SSNMR.2016.08.001 Solid State Nuclear Magnetic Resonance Creative Commons Attribution-NonCommercial-NoDerivs License http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Elsevier BV PMC |
| spellingShingle | Wang, Tuo Phyo, Pyae Hong, Mei Multidimensional solid-state NMR spectroscopy of plant cell walls |
| title | Multidimensional solid-state NMR spectroscopy of plant cell walls |
| title_full | Multidimensional solid-state NMR spectroscopy of plant cell walls |
| title_fullStr | Multidimensional solid-state NMR spectroscopy of plant cell walls |
| title_full_unstemmed | Multidimensional solid-state NMR spectroscopy of plant cell walls |
| title_short | Multidimensional solid-state NMR spectroscopy of plant cell walls |
| title_sort | multidimensional solid state nmr spectroscopy of plant cell walls |
| url | http://hdl.handle.net/1721.1/118448 https://orcid.org/0000-0002-1801-924X https://orcid.org/0000-0001-5255-5858 |
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