Generation and Isolation of Cell Cycle-arrested Cells with Complex Karyotypes
Chromosome mis-segregation leads to aneuploidy, a condition in which cells harbor an imbalanced chromosome number. Several lines of evidence strongly indicate that aneuploidy triggers genome instability, ultimately generating cells with complex karyotypes that arrest their proliferation. Isolation a...
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| Format: | Article |
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MyJoVE Corporation
2018
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| Online Access: | http://hdl.handle.net/1721.1/118619 https://orcid.org/0000-0002-1501-6190 |
| _version_ | 1826201782852780032 |
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| author | Wang, Ruoxi MacDuffie, Emily Santaguida, Stefano |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Wang, Ruoxi MacDuffie, Emily Santaguida, Stefano |
| author_sort | Wang, Ruoxi |
| collection | MIT |
| description | Chromosome mis-segregation leads to aneuploidy, a condition in which cells harbor an imbalanced chromosome number. Several lines of evidence strongly indicate that aneuploidy triggers genome instability, ultimately generating cells with complex karyotypes that arrest their proliferation. Isolation and characterization of cells harboring complex karyotypes are crucial to study the impact of an imbalanced chromosome number on cell physiology. To date, no methods have been established to reliably isolate such aneuploid cells. This paper provides a protocol for the enrichment and analysis of aneuploid cells with complex karyotypes utilizing standard, inexpensive tissue culture techniques. This protocol can be used to analyze several features of aneuploid cells with complex karyotypes including their induced senescence-associated secretory phenotype, pro-inflammatory properties, and ability to interact with immune cells. Because cancer cells often harbor imbalances in chromosome number, it is crucial to decipher how aneuploidy impacts cell physiology in normal cells, with the ultimate goal of uncovering both its pro- and anti-tumorigenic effects. |
| first_indexed | 2024-09-23T11:56:41Z |
| format | Article |
| id | mit-1721.1/118619 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T11:56:41Z |
| publishDate | 2018 |
| publisher | MyJoVE Corporation |
| record_format | dspace |
| spelling | mit-1721.1/1186192022-09-27T23:01:29Z Generation and Isolation of Cell Cycle-arrested Cells with Complex Karyotypes Wang, Ruoxi MacDuffie, Emily Santaguida, Stefano Massachusetts Institute of Technology. Department of Biology Koch Institute for Integrative Cancer Research at MIT Wang, Ruoxi MacDuffie, Emily Santaguida, Stefano Chromosome mis-segregation leads to aneuploidy, a condition in which cells harbor an imbalanced chromosome number. Several lines of evidence strongly indicate that aneuploidy triggers genome instability, ultimately generating cells with complex karyotypes that arrest their proliferation. Isolation and characterization of cells harboring complex karyotypes are crucial to study the impact of an imbalanced chromosome number on cell physiology. To date, no methods have been established to reliably isolate such aneuploid cells. This paper provides a protocol for the enrichment and analysis of aneuploid cells with complex karyotypes utilizing standard, inexpensive tissue culture techniques. This protocol can be used to analyze several features of aneuploid cells with complex karyotypes including their induced senescence-associated secretory phenotype, pro-inflammatory properties, and ability to interact with immune cells. Because cancer cells often harbor imbalances in chromosome number, it is crucial to decipher how aneuploidy impacts cell physiology in normal cells, with the ultimate goal of uncovering both its pro- and anti-tumorigenic effects. National Cancer Institute (U.S.) (David H. Koch Institute for Integrative Cancer Research at MIT. Support Core Grant P30-CA 14051) National Institutes of Health (U.S.) (Grant CA206157-22) 2018-10-19T13:37:11Z 2018-10-19T13:37:11Z 2018-04 2018-10-11T17:46:59Z Article http://purl.org/eprint/type/JournalArticle 1940-087X http://hdl.handle.net/1721.1/118619 Wang, Ruoxi W., et al. “Generation and Isolation of Cell Cycle-Arrested Cells with Complex Karyotypes.” Journal of Visualized Experiments, no. 134, Apr. 2018. © 2018, Journal of Visualized Experiments. https://orcid.org/0000-0002-1501-6190 http://dx.doi.org/10.3791/57215 Journal of Visualized Experiments Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf MyJoVE Corporation Journal of Visualized Experiments (JOVE) |
| spellingShingle | Wang, Ruoxi MacDuffie, Emily Santaguida, Stefano Generation and Isolation of Cell Cycle-arrested Cells with Complex Karyotypes |
| title | Generation and Isolation of Cell Cycle-arrested Cells with Complex Karyotypes |
| title_full | Generation and Isolation of Cell Cycle-arrested Cells with Complex Karyotypes |
| title_fullStr | Generation and Isolation of Cell Cycle-arrested Cells with Complex Karyotypes |
| title_full_unstemmed | Generation and Isolation of Cell Cycle-arrested Cells with Complex Karyotypes |
| title_short | Generation and Isolation of Cell Cycle-arrested Cells with Complex Karyotypes |
| title_sort | generation and isolation of cell cycle arrested cells with complex karyotypes |
| url | http://hdl.handle.net/1721.1/118619 https://orcid.org/0000-0002-1501-6190 |
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