Image formation of volume holographic microscopy using point spread functions
We present a theoretical formulation to quantify the imaging properties of volume holographic microscopy (VHM). Volume holograms are formed by exposure of a photosensitive recording material to the interference of two mutually coherent optical fields. Recently, it has been shown that a volume hologr...
| Main Authors: | , , , , , |
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| Format: | Article |
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SPIE
2018
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| Online Access: | http://hdl.handle.net/1721.1/118840 https://orcid.org/0000-0002-7225-7580 https://orcid.org/0000-0002-4140-1404 |
| _version_ | 1826193208055431168 |
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| author | Kou, Shan Shan Sheppard, Colin J. R. Luo, Yuan Oh, Se Baek Lee, Justin Wu Barbastathis, George |
| author2 | Massachusetts Institute of Technology. Department of Mechanical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Mechanical Engineering Kou, Shan Shan Sheppard, Colin J. R. Luo, Yuan Oh, Se Baek Lee, Justin Wu Barbastathis, George |
| author_sort | Kou, Shan Shan |
| collection | MIT |
| description | We present a theoretical formulation to quantify the imaging properties of volume holographic microscopy (VHM). Volume holograms are formed by exposure of a photosensitive recording material to the interference of two mutually coherent optical fields. Recently, it has been shown that a volume holographic pupil has spatial and spectral sectioning capability for fluorescent samples. Here, we analyze the point spread function (PSF) to assess the imaging behavior of the VHM with a point source and detector. The coherent PSF of the VHM is derived, and the results are compared with those from conventional microscopy, and confocal microscopy with point and slit apertures. According to our analysis, the PSF of the VHM can be controlled in the lateral direction by adjusting the parameters of the VH. Compared with confocal microscopes, the performance of the VHM is comparable or even potentially better, and the VHM is also able to achieve real-time and three-dimensional (3D) imaging due to its multiplexing ability. © 2010 SPIE. |
| first_indexed | 2024-09-23T09:35:40Z |
| format | Article |
| id | mit-1721.1/118840 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T09:35:40Z |
| publishDate | 2018 |
| publisher | SPIE |
| record_format | dspace |
| spelling | mit-1721.1/1188402022-09-26T12:31:50Z Image formation of volume holographic microscopy using point spread functions Kou, Shan Shan Sheppard, Colin J. R. Luo, Yuan Oh, Se Baek Lee, Justin Wu Barbastathis, George Massachusetts Institute of Technology. Department of Mechanical Engineering Luo, Yuan Oh, Se Baek Lee, Justin Wu Barbastathis, George We present a theoretical formulation to quantify the imaging properties of volume holographic microscopy (VHM). Volume holograms are formed by exposure of a photosensitive recording material to the interference of two mutually coherent optical fields. Recently, it has been shown that a volume holographic pupil has spatial and spectral sectioning capability for fluorescent samples. Here, we analyze the point spread function (PSF) to assess the imaging behavior of the VHM with a point source and detector. The coherent PSF of the VHM is derived, and the results are compared with those from conventional microscopy, and confocal microscopy with point and slit apertures. According to our analysis, the PSF of the VHM can be controlled in the lateral direction by adjusting the parameters of the VH. Compared with confocal microscopes, the performance of the VHM is comparable or even potentially better, and the VHM is also able to achieve real-time and three-dimensional (3D) imaging due to its multiplexing ability. © 2010 SPIE. 2018-11-02T17:19:38Z 2018-11-02T17:19:38Z 2010-03 2018-10-26T17:28:30Z Article http://purl.org/eprint/type/ConferencePaper http://hdl.handle.net/1721.1/118840 Luo, Yuan, et al. "Image Formation of Volume Holographic Microscopy Using Point Spread Functions." Proceedings Volume 7622, Medical Imaging 2010: Physics of Medical Imaging, 15-18 March, 2010, San Diego, California, 2010, SPIE, p. 76224E. © 2010 Society of Photo-Optical Instrumentation Engineers (SPIE) https://orcid.org/0000-0002-7225-7580 https://orcid.org/0000-0002-4140-1404 http://dx.doi.org/10.1117/12.844310 Proceedings Volume 7622, Medical Imaging 2010: Physics of Medical Imaging Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf SPIE SPIE |
| spellingShingle | Kou, Shan Shan Sheppard, Colin J. R. Luo, Yuan Oh, Se Baek Lee, Justin Wu Barbastathis, George Image formation of volume holographic microscopy using point spread functions |
| title | Image formation of volume holographic microscopy using point spread functions |
| title_full | Image formation of volume holographic microscopy using point spread functions |
| title_fullStr | Image formation of volume holographic microscopy using point spread functions |
| title_full_unstemmed | Image formation of volume holographic microscopy using point spread functions |
| title_short | Image formation of volume holographic microscopy using point spread functions |
| title_sort | image formation of volume holographic microscopy using point spread functions |
| url | http://hdl.handle.net/1721.1/118840 https://orcid.org/0000-0002-7225-7580 https://orcid.org/0000-0002-4140-1404 |
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