Towards the Development of Optogenetically-Controlled Skeletal Muscle Actuators
Engineered skeletal muscle tissue has the potential to be used as dual use actuator and stress-bearing material providing numerous degrees of freedom and with significant active stress generation. To exploit the potential features, however, technologies must be established to generate mature muscle...
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| Format: | Article |
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ASME International
2018
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| Online Access: | http://hdl.handle.net/1721.1/118892 https://orcid.org/0000-0003-3155-6223 |
| _version_ | 1811094098506940416 |
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| author | Kim, Hyeonyu Neal, Devin M Asada, Haruhiko |
| author2 | Massachusetts Institute of Technology. Department of Mechanical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Mechanical Engineering Kim, Hyeonyu Neal, Devin M Asada, Haruhiko |
| author_sort | Kim, Hyeonyu |
| collection | MIT |
| description | Engineered skeletal muscle tissue has the potential to be used as dual use actuator and stress-bearing material providing numerous degrees of freedom and with significant active stress generation. To exploit the potential features, however, technologies must be established to generate mature muscle strips that can be controlled with high fidelity. Here, we present a method for creating mature 3-D skeletal muscle tissues that contract in response to optical activation stimuli. The muscle strips are fascicle-like, consisting of several mm-long multinucleate muscle cells bundled together. We have found that applying a tension to the fascicle-like muscle tissue promotes maturation of the muscle. The fascicle-like muscle tissue is controlled with high spatiotemporal resolution based on optogenetic coding. The mouse myoblasts C2C12 were transfected with Channelrhodopsin-2 to enable light (∼470 nm) to control muscle contraction. The 3D muscle tissue not only twitches in response to an impulse light beam, but also exhibits a type of tetanus, a prolonged contraction of continuous stimuli, for the first time. In the following, the materials and culturing method used for 3D muscle generation is presented, followed by experimental results of muscle constructs and optogenetic control of the 3D muscle tissue. |
| first_indexed | 2024-09-23T15:55:04Z |
| format | Article |
| id | mit-1721.1/118892 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T15:55:04Z |
| publishDate | 2018 |
| publisher | ASME International |
| record_format | dspace |
| spelling | mit-1721.1/1188922022-09-29T17:02:08Z Towards the Development of Optogenetically-Controlled Skeletal Muscle Actuators Kim, Hyeonyu Neal, Devin M Asada, Haruhiko Massachusetts Institute of Technology. Department of Mechanical Engineering Kim, Hyeonyu Neal, Devin M Asada, Haruhiko Engineered skeletal muscle tissue has the potential to be used as dual use actuator and stress-bearing material providing numerous degrees of freedom and with significant active stress generation. To exploit the potential features, however, technologies must be established to generate mature muscle strips that can be controlled with high fidelity. Here, we present a method for creating mature 3-D skeletal muscle tissues that contract in response to optical activation stimuli. The muscle strips are fascicle-like, consisting of several mm-long multinucleate muscle cells bundled together. We have found that applying a tension to the fascicle-like muscle tissue promotes maturation of the muscle. The fascicle-like muscle tissue is controlled with high spatiotemporal resolution based on optogenetic coding. The mouse myoblasts C2C12 were transfected with Channelrhodopsin-2 to enable light (∼470 nm) to control muscle contraction. The 3D muscle tissue not only twitches in response to an impulse light beam, but also exhibits a type of tetanus, a prolonged contraction of continuous stimuli, for the first time. In the following, the materials and culturing method used for 3D muscle generation is presented, followed by experimental results of muscle constructs and optogenetic control of the 3D muscle tissue. National Science Foundation (U.S.) National Science Foundation (U.S.). Science and Technology Center. Emergent Behaviors of Integrated Cellular Systems (grant no. NSF STC-0902396) Singapore-MIT Alliance. BioSystems and Micromechanics (BioSyM) Inter-Disciplinary Research Group 2018-11-05T19:33:44Z 2018-11-05T19:33:44Z 2013-10 2018-10-23T18:21:11Z Article http://purl.org/eprint/type/ConferencePaper 978-0-7918-5613-0 http://hdl.handle.net/1721.1/118892 Kim, Hyeonyu, Devin Neal, and H. Harry Asada. “Towards the Development of Optogenetically-Controlled Skeletal Muscle Actuators.” Volume 2: Control, Monitoring, and Energy Harvesting of Vibratory Systems; Cooperative and Networked Control; Delay Systems; Dynamical Modeling and Diagnostics in Biomedical Systems; Estimation and Id of Energy Systems; Fault Detection; Flow and Thermal Systems; Haptics and Hand Motion; Human Assistive Systems and Wearable Robots; Instrumentation and Characterization in Bio-Systems; Intelligent Transportation Systems; Linear Systems and Robust Control; Marine Vehicles; Nonholonomic Systems (October 21, 2013). https://orcid.org/0000-0003-3155-6223 http://dx.doi.org/10.1115/DSCC2013-4062 Volume 2: Control, Monitoring, and Energy Harvesting of Vibratory Systems; Cooperative and Networked Control; Delay Systems; Dynamical Modeling and Diagnostics in Biomedical Systems; Estimation and Id of Energy Systems; Fault Detection; Flow and Thermal Systems; Haptics and Hand Motion; Human Assistive Systems and Wearable Robots; Instrumentation and Characterization in Bio-Systems; Intelligent Transportation Systems; Linear Systems and Robust Control; Marine Vehicles; Nonholonomic Systems Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf ASME International ASME |
| spellingShingle | Kim, Hyeonyu Neal, Devin M Asada, Haruhiko Towards the Development of Optogenetically-Controlled Skeletal Muscle Actuators |
| title | Towards the Development of Optogenetically-Controlled Skeletal Muscle Actuators |
| title_full | Towards the Development of Optogenetically-Controlled Skeletal Muscle Actuators |
| title_fullStr | Towards the Development of Optogenetically-Controlled Skeletal Muscle Actuators |
| title_full_unstemmed | Towards the Development of Optogenetically-Controlled Skeletal Muscle Actuators |
| title_short | Towards the Development of Optogenetically-Controlled Skeletal Muscle Actuators |
| title_sort | towards the development of optogenetically controlled skeletal muscle actuators |
| url | http://hdl.handle.net/1721.1/118892 https://orcid.org/0000-0003-3155-6223 |
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