Calcium-dependent molecular fMRI using a magnetic nanosensor
Calcium ions are ubiquitous signalling molecules in all multicellular organisms, where they mediate diverse aspects of intracellular and extracellular communication over widely varying temporal and spatial scales1. Though techniques to map calcium-related activity at a high resolution by optical mea...
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| פורמט: | Article |
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Nature Publishing Group
2018
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| גישה מקוונת: | http://hdl.handle.net/1721.1/118936 https://orcid.org/0000-0003-2776-9509 https://orcid.org/0000-0002-5044-369X https://orcid.org/0000-0002-4751-2252 https://orcid.org/0000-0002-1701-325X https://orcid.org/0000-0003-2442-5671 https://orcid.org/0000-0002-2834-6359 |
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| author | Okada, Satoshi Li, Nan Breton-Provencher, Vincent Lee, Jiyoung J Rodriguez Vargas, Elisenda Melican, James Sur, Mriganka Jasanoff, Alan Pradip Bartelle, Benjamin B. |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Okada, Satoshi Li, Nan Breton-Provencher, Vincent Lee, Jiyoung J Rodriguez Vargas, Elisenda Melican, James Sur, Mriganka Jasanoff, Alan Pradip Bartelle, Benjamin B. |
| author_sort | Okada, Satoshi |
| collection | MIT |
| description | Calcium ions are ubiquitous signalling molecules in all multicellular organisms, where they mediate diverse aspects of intracellular and extracellular communication over widely varying temporal and spatial scales1. Though techniques to map calcium-related activity at a high resolution by optical means are well established, there is currently no reliable method to measure calcium dynamics over large volumes in intact tissue2. Here, we address this need by introducing a family of magnetic calcium-responsive nanoparticles (MaCaReNas) that can be detected by magnetic resonance imaging (MRI). MaCaReNas respond within seconds to [Ca2+] changes in the 0.1-1.0 mM range, suitable for monitoring extracellular calcium signalling processes in the brain. We show that the probes permit the repeated detection of brain activation in response to diverse stimuli in vivo. MaCaReNas thus provide a tool for calcium-activity mapping in deep tissue and offer a precedent for the development of further nanoparticle-based sensors for dynamic molecular imaging with MRI. |
| first_indexed | 2024-09-23T16:41:21Z |
| format | Article |
| id | mit-1721.1/118936 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T16:41:21Z |
| publishDate | 2018 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/1189362023-02-26T02:29:29Z Calcium-dependent molecular fMRI using a magnetic nanosensor Okada, Satoshi Li, Nan Breton-Provencher, Vincent Lee, Jiyoung J Rodriguez Vargas, Elisenda Melican, James Sur, Mriganka Jasanoff, Alan Pradip Bartelle, Benjamin B. Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Massachusetts Institute of Technology. Department of Nuclear Science and Engineering Picower Institute for Learning and Memory Okada, Satoshi Bartelle, Benjamin Li, Nan Breton-Provencher, Vincent Lee, Jiyoung J Rodriguez Vargas, Elisenda Melican, James Sur, Mriganka Jasanoff, Alan Pradip Calcium ions are ubiquitous signalling molecules in all multicellular organisms, where they mediate diverse aspects of intracellular and extracellular communication over widely varying temporal and spatial scales1. Though techniques to map calcium-related activity at a high resolution by optical means are well established, there is currently no reliable method to measure calcium dynamics over large volumes in intact tissue2. Here, we address this need by introducing a family of magnetic calcium-responsive nanoparticles (MaCaReNas) that can be detected by magnetic resonance imaging (MRI). MaCaReNas respond within seconds to [Ca2+] changes in the 0.1-1.0 mM range, suitable for monitoring extracellular calcium signalling processes in the brain. We show that the probes permit the repeated detection of brain activation in response to diverse stimuli in vivo. MaCaReNas thus provide a tool for calcium-activity mapping in deep tissue and offer a precedent for the development of further nanoparticle-based sensors for dynamic molecular imaging with MRI. National Institutes of Health (U.S.) (Grant R01-DA038642) National Institutes of Health (U.S.) (Grant DP2-OD2114) National Institutes of Health (U.S.) (Grant U01-NS090451) National Institutes of Health (U.S.) (Grant R01-EY007023) National Science Foundation (U.S.) (Grant 0070319) National Institutes of Health (U.S.) (Grant S10-OD016326) 2018-11-07T15:22:56Z 2018-11-07T15:22:56Z 2018-04 2016-10 2018-11-06T14:24:34Z Article http://purl.org/eprint/type/JournalArticle 1748-3387 1748-3395 http://hdl.handle.net/1721.1/118936 Okada, Satoshi et al. “Calcium-Dependent Molecular fMRI Using a Magnetic Nanosensor.” Nature Nanotechnology 13, 6 (April 2018): 473–477 © 2018 The Author(s) https://orcid.org/0000-0003-2776-9509 https://orcid.org/0000-0002-5044-369X https://orcid.org/0000-0002-4751-2252 https://orcid.org/0000-0002-1701-325X https://orcid.org/0000-0003-2442-5671 https://orcid.org/0000-0002-2834-6359 http://dx.doi.org/10.1038/s41565-018-0092-4 Nature Nanotechnology Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Nature Publishing Group PMC |
| spellingShingle | Okada, Satoshi Li, Nan Breton-Provencher, Vincent Lee, Jiyoung J Rodriguez Vargas, Elisenda Melican, James Sur, Mriganka Jasanoff, Alan Pradip Bartelle, Benjamin B. Calcium-dependent molecular fMRI using a magnetic nanosensor |
| title | Calcium-dependent molecular fMRI using a magnetic nanosensor |
| title_full | Calcium-dependent molecular fMRI using a magnetic nanosensor |
| title_fullStr | Calcium-dependent molecular fMRI using a magnetic nanosensor |
| title_full_unstemmed | Calcium-dependent molecular fMRI using a magnetic nanosensor |
| title_short | Calcium-dependent molecular fMRI using a magnetic nanosensor |
| title_sort | calcium dependent molecular fmri using a magnetic nanosensor |
| url | http://hdl.handle.net/1721.1/118936 https://orcid.org/0000-0003-2776-9509 https://orcid.org/0000-0002-5044-369X https://orcid.org/0000-0002-4751-2252 https://orcid.org/0000-0002-1701-325X https://orcid.org/0000-0003-2442-5671 https://orcid.org/0000-0002-2834-6359 |
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