Caring for cells in microsystems: principles and practices of cell-safe device design and operation
Microfluidic device designers and users continually question whether cells are 'happy' in a given microsystem or whether they are perturbed by micro-scale technologies. This issue is normally brought up by engineers building platforms, or by external reviewers (academic or commercial) comp...
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| Format: | Article |
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Royal Society of Chemistry (RSC)
2018
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| Online Access: | http://hdl.handle.net/1721.1/119013 https://orcid.org/0000-0002-3690-519X https://orcid.org/0000-0001-8898-2296 |
| _version_ | 1826202085958352896 |
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| author | Varma, Sarvesh Voldman, Joel |
| author2 | Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science |
| author_facet | Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Varma, Sarvesh Voldman, Joel |
| author_sort | Varma, Sarvesh |
| collection | MIT |
| description | Microfluidic device designers and users continually question whether cells are 'happy' in a given microsystem or whether they are perturbed by micro-scale technologies. This issue is normally brought up by engineers building platforms, or by external reviewers (academic or commercial) comparing multiple technological approaches to a problem. Microsystems can apply combinations of biophysical and biochemical stimuli that, although essential to device operation, may damage cells in complex ways. However, assays to assess the impact of microsystems upon cells have been challenging to conduct and have led to subjective interpretation and evaluation of cell stressors, hampering development and adoption of microsystems. To this end, we introduce a framework that defines cell health, describes how device stimuli may stress cells, and contrasts approaches to measure cell stress. Importantly, we provide practical guidelines regarding device design and operation to minimize cell stress, and recommend a minimal set of quantitative assays that will enable standardization in the assessment of cell health in diverse devices. We anticipate that as microsystem designers, reviewers, and end-users enforce such guidelines, we as a community can create a set of essential principles that will further the adoption of such technologies in clinical, translational and commercial applications. |
| first_indexed | 2024-09-23T12:01:39Z |
| format | Article |
| id | mit-1721.1/119013 |
| institution | Massachusetts Institute of Technology |
| last_indexed | 2024-09-23T12:01:39Z |
| publishDate | 2018 |
| publisher | Royal Society of Chemistry (RSC) |
| record_format | dspace |
| spelling | mit-1721.1/1190132022-10-01T07:42:39Z Caring for cells in microsystems: principles and practices of cell-safe device design and operation Varma, Sarvesh Voldman, Joel Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Varma, Sarvesh Voldman, Joel Microfluidic device designers and users continually question whether cells are 'happy' in a given microsystem or whether they are perturbed by micro-scale technologies. This issue is normally brought up by engineers building platforms, or by external reviewers (academic or commercial) comparing multiple technological approaches to a problem. Microsystems can apply combinations of biophysical and biochemical stimuli that, although essential to device operation, may damage cells in complex ways. However, assays to assess the impact of microsystems upon cells have been challenging to conduct and have led to subjective interpretation and evaluation of cell stressors, hampering development and adoption of microsystems. To this end, we introduce a framework that defines cell health, describes how device stimuli may stress cells, and contrasts approaches to measure cell stress. Importantly, we provide practical guidelines regarding device design and operation to minimize cell stress, and recommend a minimal set of quantitative assays that will enable standardization in the assessment of cell health in diverse devices. We anticipate that as microsystem designers, reviewers, and end-users enforce such guidelines, we as a community can create a set of essential principles that will further the adoption of such technologies in clinical, translational and commercial applications. National Institutes of Health (U.S.) (Grant GM090194) 2018-11-14T18:53:59Z 2018-11-14T18:53:59Z 2018-10 2018-07 2018-11-14T13:55:23Z Article http://purl.org/eprint/type/JournalArticle 1473-0197 1473-0189 http://hdl.handle.net/1721.1/119013 Varma, Sarvesh and Joel Voldman. “Caring for Cells in Microsystems: Principles and Practices of Cell-Safe Device Design and Operation.” Lab on a Chip 18, 22 (2018): 3333–3352 © 2018 The Royal Society of Chemistry https://orcid.org/0000-0002-3690-519X https://orcid.org/0000-0001-8898-2296 http://dx.doi.org/10.1039/c8lc00746b Lab on a Chip Creative Commons Attribution-NonCommercial 3.0 Unported https://creativecommons.org/licenses/by-nc/3.0/ application/pdf Royal Society of Chemistry (RSC) Royal Society of Chemistry |
| spellingShingle | Varma, Sarvesh Voldman, Joel Caring for cells in microsystems: principles and practices of cell-safe device design and operation |
| title | Caring for cells in microsystems: principles and practices of cell-safe device design and operation |
| title_full | Caring for cells in microsystems: principles and practices of cell-safe device design and operation |
| title_fullStr | Caring for cells in microsystems: principles and practices of cell-safe device design and operation |
| title_full_unstemmed | Caring for cells in microsystems: principles and practices of cell-safe device design and operation |
| title_short | Caring for cells in microsystems: principles and practices of cell-safe device design and operation |
| title_sort | caring for cells in microsystems principles and practices of cell safe device design and operation |
| url | http://hdl.handle.net/1721.1/119013 https://orcid.org/0000-0002-3690-519X https://orcid.org/0000-0001-8898-2296 |
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