Engineering Engineering Hyperthermostable rcSso7d as Reporter Molecule for in Vitro Diagnostic Tests
The hyperthermostable binding scaffold, rcSso7d, has recently been studied for use in in vitro diagnostic tests but has not yet been demonstrated as a soluble reporter protein. Here, we investigated several rcSso7d protein constructs and biotin conjugation methods to link a detectable signal to the...
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
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Royal Society of Chemistry (RSC)
2018
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| Online Access: | http://hdl.handle.net/1721.1/119208 https://orcid.org/0000-0001-8120-538X https://orcid.org/0000-0002-6217-3922 https://orcid.org/0000-0002-7096-138X |
| _version_ | 1826195556029956096 |
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| author | Sung, Ki-Joo Miller, Eric Alexander Sikes Johnson, Hadley |
| author2 | Massachusetts Institute of Technology. Department of Chemical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Chemical Engineering Sung, Ki-Joo Miller, Eric Alexander Sikes Johnson, Hadley |
| author_sort | Sung, Ki-Joo |
| collection | MIT |
| description | The hyperthermostable binding scaffold, rcSso7d, has recently been studied for use in in vitro diagnostic tests but has not yet been demonstrated as a soluble reporter protein. Here, we investigated several rcSso7d protein constructs and biotin conjugation methods to link a detectable signal to the presence of a target biomarker. |
| first_indexed | 2024-09-23T10:14:34Z |
| format | Article |
| id | mit-1721.1/119208 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T10:14:34Z |
| publishDate | 2018 |
| publisher | Royal Society of Chemistry (RSC) |
| record_format | dspace |
| spelling | mit-1721.1/1192082022-09-30T19:52:25Z Engineering Engineering Hyperthermostable rcSso7d as Reporter Molecule for in Vitro Diagnostic Tests Sung, Ki-Joo Miller, Eric Alexander Sikes Johnson, Hadley Massachusetts Institute of Technology. Department of Chemical Engineering Sung, Ki-Joo Miller, Eric Alexander Sikes Johnson, Hadley The hyperthermostable binding scaffold, rcSso7d, has recently been studied for use in in vitro diagnostic tests but has not yet been demonstrated as a soluble reporter protein. Here, we investigated several rcSso7d protein constructs and biotin conjugation methods to link a detectable signal to the presence of a target biomarker. National Science Foundation (U.S.) (Grant 1122374) 2018-11-19T19:35:53Z 2018-11-19T19:35:53Z 2018-10 2018-07 Article http://purl.org/eprint/type/JournalArticle 2058-9689 http://hdl.handle.net/1721.1/119208 Sung, Ki-Joo et al. “Engineering Hyperthermostable rcSso7d as Reporter Molecule for in Vitro Diagnostic Tests.” Molecular Systems Design & Engineering 2018, 5 (October 2018) © 2018 Royal Society of Chemistry https://orcid.org/0000-0001-8120-538X https://orcid.org/0000-0002-6217-3922 https://orcid.org/0000-0002-7096-138X en_US http://dx.doi.org/ 10.1039/C8ME00049B Molecular Systems Design & Engineering Creative Commons Attribution-NonCommercial 3.0 Unported https://creativecommons.org/licenses/by-nc/3.0/ application/pdf Royal Society of Chemistry (RSC) Royal Society of Chemistry |
| spellingShingle | Sung, Ki-Joo Miller, Eric Alexander Sikes Johnson, Hadley Engineering Engineering Hyperthermostable rcSso7d as Reporter Molecule for in Vitro Diagnostic Tests |
| title | Engineering Engineering Hyperthermostable rcSso7d as Reporter Molecule for in Vitro Diagnostic Tests |
| title_full | Engineering Engineering Hyperthermostable rcSso7d as Reporter Molecule for in Vitro Diagnostic Tests |
| title_fullStr | Engineering Engineering Hyperthermostable rcSso7d as Reporter Molecule for in Vitro Diagnostic Tests |
| title_full_unstemmed | Engineering Engineering Hyperthermostable rcSso7d as Reporter Molecule for in Vitro Diagnostic Tests |
| title_short | Engineering Engineering Hyperthermostable rcSso7d as Reporter Molecule for in Vitro Diagnostic Tests |
| title_sort | engineering engineering hyperthermostable rcsso7d as reporter molecule for in vitro diagnostic tests |
| url | http://hdl.handle.net/1721.1/119208 https://orcid.org/0000-0001-8120-538X https://orcid.org/0000-0002-6217-3922 https://orcid.org/0000-0002-7096-138X |
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